2024-11-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115112784
External Id
Institute of Immunobiology, Kantonsspital St. Gallen, St.Gallen, Switzerland
INSDC center name
2024-01-17T21:05:32Z
INSDC last update
public
INSDC status
ERS17748526
SRA accession
E-MTAB-13710:Sample 3
Submitter Id
75
year
age
ArrayExpress
broker name
fibroblast, endothelial cell
cell_type
not collected
collection date
human
common name
Protocols: Samples were collected after surgical resection of lung lobe or wedges from patients. Fresh surgical specimens of NSCLC and unaffected autologous lung tissue were mechanically disaggregated and tissue was digested using a MACS human tumor dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol. Hematopoietic, erythroid and tumor cells were depleted by incubating the cell suspension with MACS anti-CD45, anti-CD235a (Glycophorin A) and anti-CD326 (EpCAM) MicroBeads (Miltenyi Biotec) and passing through a MACS LS column (Miltenyi Biotec) to enrich for stromal cells. The flow-through was centrifuged and single cell suspension was stained for further flow cytometric analysis or cell sorting. The unbound single cell suspension was stained for cell sorting with a BD FACS Melody 2-way or 4-way cell sorter (BD Biosciences) and run on the 10x Chromium analyzer (10X Genomics). For the isolation of immune cells, tissues were gently smashed through a mesh using a syringe plunger and washed with RMPI1640 containing 2% FCS and 20 mM HEPES (Sigma-Aldrich) before staining for flow cytometric analysis or cell sorting. cDNA library generation was performed following the established commercial protocol for Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3/3.1 Chemistry.
description
adult
developmental stage
non-small cell lung adenocarcinoma
disease
not collected
geographic location (country and/or sea)
Adenocarcinoma 02
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung tumor subpleural margin
organism part
Homo sapiens
scientific_name
female
sex
Sample 3
title