2024-11-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114382248
External Id
Tel Aviv university
INSDC center name
2023-09-13T16:00:17Z
INSDC last update
public
INSDC status
ERS16371345
SRA accession
E-MTAB-13279_2:C_bat10_12
Submitter Id
ArrayExpress
broker name
not collected
collection date
Egyptian rousette
common name
Protocols: Gut sections were frozen in OCT according to 10X Genomics Visium protocol. Then 10x Genomics Visium protocol was applied on the (OCT)-embedded fresh frozen samples. All tissues were sectioned using a Leica CX3050S cryostat and were cut to obtain a 10 µm width. Samples were then tested for RNA integrity using a Tapestation 4200. Only blocks with RIN above 7 were used. Tissue optimization was performed, resulting in a permeabilization time of 23 minutes The Visium spatial gene expression protocol from 10X Genomics was applied by loading a total of 11 gut sections from two individual bats onto the Library Preparation Slide (4 grid sections) and following the manufacturer’s protocol cDNA library preparation was were carried out according to the manufacturer’s protocol.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
bat10
individual
not applicable
isolate
Rousettus aegyptiacus
organism
Rousettus aegyptiacus
organism
intestine
organism part
Rousettus aegyptiacus
scientific_name
male
sex
C_bat10_12
title