2024-11-22
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Cardiovascular Regeneration
INSDC center name
public
INSDC status
ERS22444140
SRA accession
E-MTAB-14589_2:Sample 6
Submitter Id
10
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: To analyze the cardiac transcriptome on single nuclear level, myocardium was collected from explanted hearts of healthy controls (n = 3) or mice with HFpEF either untreated (n = 3) or treated with LNA-92a (n = 4) or LNA-control (n = 4) after 10 weeks of HFD/L-NAME exposure or chow diet. All procedures involving the use and care of animals were performed according to the German animal protection code. Approval was granted by the local state authorities (state of Hessen). To induce HFpEF, male C57Bl/6J wild-type mice from Janvier (Le Genest Saint Isle, 239 France) and from Charles River (Sulzfeld, Germany) were subjected to a combination of high-fat diet (HFD) (60% kilocalories from fat (lard)) and N[w]-nitro-l-arginine methyl ester (L-NAME; 0.5 g/l in drinking water, Sigma-Aldrich) for 10 weeks Following two weeks of HFpEF induction, locked nucleic acid (LNA)-modified antisense oligonucleotides-92a (1.5 mg/kg BW weekly) or LNA-control were injected intraperitoneal, which are directed to inhibit miR-92a-3p (miRagen Therapeutics) Tissue was lysed in Qiazol and RNA isolated using Qiagen miRNeasy Mini Kits following the manufactures protocol. Expression analysis of miR-92a-3p was performed using Taqman Assays primers and relative miR-92a-3p expression was calculated using 2−ΔCT Library quantification and quality assessment was performed using Bioanalyzer Agilent 2100 using a High Sensitivity DNA chip (5067-4627 Agilent Genomics).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
10 weeks of HFD/L-NAME
growth condition
6
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6J
strain
Sample 6
title