2024-11-22
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS22448189
SRA accession
E-MTAB-14638:13_RNA_TAb09-1
Submitter Id
9 to 11
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Male mice were randomly assigned to one of three treatment groups: Control (CON), non-absorbable antibiotics (nABX), or low protein high sugar diet (LPHS). Young (3 weeks) and mature (12-13 months) mice in the CON group received water and standard rodent diet, nABX mice received antibiotics in their water (Neomycin, Bacitracin, Pimaricin) along with the standard diet, and LPHS mice were given a low-protein, high-sugar (LPHS) diet and water. Whole testes from FVB and C57BL/6J control and treated mice were snap-frozen in liquid nitrogen and stored at -80C. They were homogenized in Trizol with a BeadBug[TM] homogenizer, and total RNA was extracted following the manufacturer's protocol. RNA was quantified with a Nanodrop and RNA TapeStation kit. RNA-seq libraries were prepared using 100-500ng of total RNA with the NEBNext Ultra II directional RNA kit and amplified by 11 PCR cycles.
description
not collected
geographic location (country and/or sea)
birth
initial time point
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testis
organism part
Mus musculus
scientific_name
C57BL/6J
strain
13_RNA_TAb09-1
title
nABX
treatment