2024-11-26
ENA first public
ERC000011
ENA-CHECKLIST
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
INSDC center name
public
INSDC status
ERS21101797
SRA accession
E-MTAB-14482:Sample 1
Submitter Id
ArrayExpress
broker name
MAIT
cell_type
not collected
collection date
human
common name
Protocols: De-identified intestinal tissues were collected. Relative normal tissues from patients with colorectal cancer were obtained and stored in cold SPS-1 storage buffer before immediate processing.  For isolation of lymphocytes from intestines, intestine tissues were cut into small pieces, and epithelial cells were dissociated with HBSS containing 5 mM EDTA, 1 mM DTT, and 2% FBS, shaken for 20 minutes at 37°C. This process was performed twice. After each step, samples were vortexed, rinsed with PBS, and the epithelial cell layer was discarded. The remaining tissue was digested in HBSS with 1 mg/mL Liberase TM, and 20 μg/mL DNase I for 30 minutes at 37°C. Percoll centrifugation was performed to remove dead cells and debris. The resulting single cells were strained through a 70 μM mesh.  MAIT cells were sorted by fluorescence activated cell sorting (FACS) after surface marker staining. cDNA-seq libraries were prepared using the chromium 5’ single cell gene expression kit from 10x genomics according to the manufacturer’s instructions.
description
colorectal cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
large intestine
organism part
2
replicate
Homo sapiens
scientific_name
Sample 1
title