2024-11-29
ENA first public
ERC000024
ENA-CHECKLIST
Scottish Microbiology Reference Laboratories
INSDC center name
public
INSDC status
ERS22138814
SRA accession
16_TD2
Submitter Id
0.03
L
amount or size of sample collected
ENVO:00000873
broad-scale environmental context
2017-12-13
collection date
0.1
m
depth
Pond water filtered through 0.4um polycarbonate membrane
description
0.01176
µmol/kg
dissolved oxygen
ENVO:00002228
environmental medium
Bangladesh
geographic location (country and/or sea)
90.45
DD
geographic location (latitude)
24.79
DD
geographic location (longitude)
Tarakanda:Gopalpur
geographic location (region and locality)
ENVO:00000033
local environmental context
CTAB DNA extraction protocol, dx.doi.org/10.17504/protocols.io.bw8gphtw
nucleic acid extraction
aquaculture metagenome
organism
7.4
pH
16S.515fB:GTGYCAGCMGCCGCGGTAA, 16S.806rB:GGACTACNVGGGTWTCTAAT, 18S.1391f:GTACACACCGCCCGTC, 18S.EukB:TGATCCTTCTGCAGGTTCACCTAC
pcr primers
Microbiomes of small-scale finfish aquaculture ponds in Bangladesh
project name
174
psu
salinity
aquaculture metagenome
scientific_name
Illumina MiSeq
sequencing method
0.4
µm
size-fraction lower threshold
16S rRNA/18S rRNA
target gene
V4/V9
target subfragment
27.4
ºC
temperature
Bangladesh aquaculture pond water, Dec 2017 (winter 2), tilapia pond TD, sample 1
title