2024-11-29
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114910886
External Id
Novo Nordist Foundation Center for Stem Cell Medicine (reNEW) and Novo Nordisk Center for Protein Research (CPR)
INSDC center name
2023-12-12T11:59:41Z
INSDC last update
public
INSDC status
ERS17355837
SRA accession
E-MTAB-13618:Pbx1KO_clone15_Lat_Meso
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Cells were treated with accutase and counted. Approximately 1 million cells per sample were lysed. For all conditions, at least two biological replicate samples were collected from independent experiments. Total RNA was extracted using the RNeasy Mini Kit according to the manufacturer’s instructions (Qiagen). RNA concentration was quantified by NanoDrop, and quality was verified with a Fragment analyzer. Library preparation was carried out using 0.5 μg of RNA with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, E7770S) as per the manufacturer’s instructions. Libraries were amplified for 5 PCR cycles and purified with AMPure XP beads. DNA concentration was measured with a Qubit Fluorometer, and fragment size was determined with a Fragment analyzer.
description
EpiSCs + 24h towards PPS + 24h towards ExM
developmental stage
Pbx1-KO (homozygous)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
129S6/SvEvTac
strain
Pbx1KO_clone15_Lat_Meso
title