2024-11-29
ENA first public
ERC000011
ENA-CHECKLIST
Inselspital, Bern University Hospital, Bern, Switzerland
INSDC center name
public
INSDC status
ERS21005169
SRA accession
E-MTAB-14444:23_GW9662
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Human naive T cells were isolated from PBMCs using the EasySep™ Human naive CD4+ T Cell Isolation Kit (Stemcell Technologies) as per the manufacturer’s instructions. Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 50 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96 well plate in a total volume of 200μL cell culture medium. Th9 cells were incubated with GW9662 for 48 hours. Total RNA was isolated using the RNAeasy kit from Qiagen. Illumina TruSeq Stranded mRNA protocol (Directional, first strand)
description
normal
disease
not collected
geographic location (country and/or sea)
BC23
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
23_GW9662
title