2024-11-30
ENA first public
ERC000011
ENA-CHECKLIST
School of Biological Sciences, Nanyang Technological University
INSDC center name
public
INSDC status
ERS22133931
SRA accession
E-MTAB-14605:Col-0_rep2
Submitter Id
12 days after flowering (DAF)
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Protocols: Total RNA were extracted from pooled Arabidopsis siliques 12 days after flowering (DAF).  Arabidopsis thaliana seedlings are grown in growth chambers under constrant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1  illumination)/8 h dark. Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer’s protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instruction
description
Col-0
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
Siliques
organism part
Arabidopsis thaliana
scientific_name
Col-0_rep2
title