2024-11-30
ENA first public
ERC000011
ENA-CHECKLIST
ICL-CFB
INSDC center name
public
INSDC status
ERS22133788
SRA accession
E-MTAB-14602:Sample 9
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Total RNA was isolated from cells with Trizol (Thermofisher Sci 15596018) on ice. Left ventricle (LV) free-wall tissue was minced into small chunks (1-2 mm3) and then digested in 0.05% Trypsin-EDTA (#25300054) for 2 min. Tissue chunks were then transferred into fibronectin-coated dishes (F1141) and cultured in DMEM supplemented with 20% FBS and 1% antibiotic-antimycotic at 37°C and 5% CO2. When confluent, fibroblasts were harvested from the dishes and used for experiments. Cultures were pre-treated for 24 hrs with UDCA before stimulation with 5ng/ml human IL-11 (PHC0115, Life Technologies) for a further 24 hrs.  Samples were purified with Monarch RNA Clean up kit (NEB, #T2030), including treatment with DNase I to eliminate any DNA contamination Purified RNA was given to the Imperial Genomics Facility who prepared the library and performed the sequencing experiments.  mRNA was selected by polyA selection and rRNA was depleted. Libraries were prepared using the TruSeq Stranded mRNACoda Sample Prep Kit (Illumina, California, USA).
description
dilated cardiomyopathy
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
heart left ventricle
organism part
Homo sapiens
scientific_name
Sample 9
title