2024-11-30
ENA first public
ERC000015
ENA-CHECKLIST
SAMEA112177605
External Id
Max Rubner-Institute
INSDC center name
2022-11-30T08:17:17Z
INSDC last update
public
INSDC status
ERS14287445
SRA accession
ZW21_du4
Submitter Id
fwd:AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGT::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNNNAAGTCGAGAGTCAGTCAGCC
adapters
0.2
g
amount or size of sample collected
ENVO_01001033:digestive tract environment
broad-scale environmental context
2021-11-18
collection date
stool sample, native
description
ENVO_00002003:fecal material
environmental medium
Germany
geographic location (country and/or sea)
49.0139822
DD
geographic location (latitude)
8.4240071
DD
geographic location (longitude)
stool
host body product
P2
host subject id
human-gut
human gut environmental package
metagenome
investigation type
ENVO_2100002:intestine environment
local environmental context
PCR
nucleic acid amplification
Nucleospin Stool Kit (Macherey Nagel)
nucleic acid extraction
human gut metagenome
organism
initial denaturation:98degC_2min; 25 cycles: denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_15s; final_extension:72degC_5min
pcr conditions
fwd:GTGYCAGCMGCCGCGGTAA::rev:GGACTACNVGGGTWTCTAAT
pcr primers
ZWoMi_study
project name
freezing
sample material processing
-80
°C
sample storage temperature
human gut metagenome
scientific_name
Illumina MiSeq v3
sequencing method
ZW21
stool sample id
16S-rRNA
target gene
V4
target subfragment
ZWoMi087
title