2024-11-30
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Cardiovascular Regeneration
INSDC center name
public
INSDC status
ERS22058310
SRA accession
E-MTAB-14595:Sample 3
Submitter Id
18
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Aged male C57Bl/6J wildtype mice were purchased from Janvier (Le Genest SaintIsle, France) and from Charles River (Sulzfeld, Germany). Homozygosity of these inbred mice was controlled by Janvier and Charles River using exome sequencing.  To overexpress VEGFB186 in aged mouse hearts, AAV9 vectors carrying the VEGFB186 gene under the control of the CAG promotor were used. Non targeting AAV9 particles served as negative control. Both viruses were provided by K.A and I.S. The viral particles were injected intraperitoneally, at a concentration of 2.2 x 1011 viral particles per animal to 18-month-old mice. Before AAV9-treatment (baseline) and 4 and 8 weeks after the treatment, echocardiography was used to monitor the systolic and diastolic function of each mouse.  To isolate total RNA from cardiac tissue, hearts combined with 700µl of Qiazol and ¼” ceramic spheres and were homogenized three times for 20 seconds (4 min/s). The miRNeasy Mini kit (217004, Qiagen) was used for further steps of RNA isolation. The RNA concentration was determined by measuring absorption at 260 nm and 280 nm with the NanoDrop ND 2000-spectrophotometer (PeqLab). Library quantification and quality assessment was performed using Bioanalyzer Agilent 2100 using a High Sensitivity DNA chip (5067-4627 Agilent Genomics).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
559
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6
strain
Sample 3
title