2024-12-02
ENA first public
ERC000011
ENA-CHECKLIST
Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin, Versailles, France
INSDC center name
public
INSDC status
ERS20963280
SRA accession
E-MTAB-14435:Fi_Tet9_M1
Submitter Id
3
week
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col-0 (CS8846) x Ler-1  (CS8845)
cultivar
Protocols: Parental Col-0 and Ler-1 and F1 growth: A. thaliana plants were grown in greenhouses with 70% humidity and under a 16 h/8 h day/night photoperiod with temperatures of 19 °C during the day and 16 °C at night.  Wild-type Col-0 and Ler-1 are 186AV1B4 and 213AV1B1 from the Versailles A. thaliana stock centre (http://publiclines.versailles.inra.fr/).The following mutations were used in this study: in Col-0, fancm-1 4, figl1-16, recq4a-4 (N419423) and recq4b-2 (N511130) 28, apt1-3 20, qrt1-2 (CS8846)18; in Ler-1, fancm-10 6, figl1-12 6,  recq4a-W387X 5, and qrt1-1 (CS8845)18. Flowers from the F1 obtained by crossing CS8846 and CS8845 were tapped on a glass slide to deposit tetrads of pollen grains. Using a needle, a single tetrad was taken and gently embedded in the stigma of a apt1-3 Col-0 plant. The fertilized plant was grown in a greenhouse without other Arabidopsis plants to avoid cross-pollinization. Seeds from fruits having exactly four seeds were sterilized a few seconds in a drop of 70% ethanol and sown on solid medium 29  without sugar.  After stratification for 2 days at 4◦C, plants were grown in long day conditions. At the four leaves stage, the plants were transferred on soil and grown in green houses until the induction of the first hamp.   Illumina TruSeq
description
rosette growth stage
developmental stage
figl1 mutant
genotype
not collected
geographic location (country and/or sea)
Fi_Tet9
individual
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
rosette leaf
organism part
2
replicate
NA
run
Arabidopsis thaliana
scientific_name
Fi_Tet9_M1
title