2024-12-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115048332
External Id
University of Silesia, Katowice, Poland
INSDC center name
2023-12-19T20:00:12Z
INSDC last update
public
INSDC status
ERS17585994
SRA accession
E-MTAB-13641:WT, leaf 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: For RNA-isolation analyses, plant tissue (shoot and root) was collected in four biological replicates, each containing tissue from 4 seedlings. For the RNAseq experiment, plants were grown in hydroponic conditions up to 21 days. Six plants were placed in the 1.5 l container filled with ½ Hoagland solution (Hoagland and Dennis, 1938). The medium was replaced every week. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity. Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560). Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China).
description
seedling development stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Hordeum vulgare
organism
Hordeum vulgare
organism
shoot
organism part
Hordeum vulgare
scientific_name
WT, leaf 1
title