2024-12-06
ENA first public
ERC000011
ENA-CHECKLIST
Genentech
INSDC center name
public
INSDC status
ERS21412224
SRA accession
E-MTAB-14590:CD45_3
Submitter Id
ArrayExpress
broker name
CD45+
cell_type
not collected
collection date
house mouse
common name
Protocols: We sorted live, CD45+ cells from single-cell suspensions of tumors from 3 mice using a FACSAria (BD Biosciences). Additionally, T cells from 4 mice were isolated (live, CD45+, TCRb+). Gene expression and TCR libraries were generated using the Chromium Single Cell 5′ Library and V(D)J Reagent Kit (10X Genomics) according to manufacturer’s recommendations. 20,000 cells per sample were loaded into each channel of the Chromium Chip, and recommendations were followed assuming targeted cell recovery of 2,000–10,000 cells. 10X Genomics single-cell gene expression libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and profiled using the Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies).
description
adult
developmental stage
not collected
geographic location (country and/or sea)
3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
tumor
organism part
Mus musculus
scientific_name
female
sex
C57BL/6J
strain
CD45_3
title