2024-12-09
ENA first public
ERC000011
ENA-CHECKLIST
School of Infection & Immunity, University of Glasgow
INSDC center name
public
INSDC status
ERS20302135
SRA accession
E-MTAB-14192:SA271_ST
Submitter Id
77
year
age
ArrayExpress
broker name
all synovial celltypes
cell_type
not collected
collection date
human
common name
Protocols: To investigate synovial tissue myeloid DC and T-cell heterogeneity in RA, we performed scRNAseq on matched peripheral blood mononuclear cells (PBMC) and synovial tissue (ST) biopsies (n=3). Synovial biopsies were taken using 14G Precisa Needle (HS Hospital Service, Italy) in ultrasound-guided protocol, and digested as described previously. All live cells or CD45pos immune cells, were FACS sorted, after exclusion of dead cells with Fixable Viability Dye eFluor® 780 (eBiosciences). Maximum 20,000 immune cells from blood or tissue cells were sorted into Protein LoBind 1.5 ml Eppendorf tube containing 300 μl of RPMI media with 10% of FSC. Cells were loaded onto a Chromium Controller (10X Genomics) for single-cell partitioning, followed by library preparation using Single-Cell 3’ Reagent Kits v2.  As recommended by 10X Genomics protocol for the 10X Single Cell 3’ Reagent Kit v2 As recommended by 10X Genomics protocol for the 10X Single Cell 3’ Reagent Kit v2
description
adult
developmental stage
rheumatoid arthritis
disease
not collected
geographic location (country and/or sea)
SA271
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Naive to treatment
response to treatment
Homo sapiens
scientific_name
female
sex
SA271_ST
title