2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22193110
SRA accession
SRP262948_co1_5
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
MetaBAT2;2.15;default
binning software
engineered
broad-scale environmental context
2016-06-21
collection date
88.7
%
completeness score
CheckM2;1.0.2;default
completeness software
2.15
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR11830853,SRR11830854 of study SRP262948.
description
engineered
environmental medium
China
geographic location (country and/or sea)
22.59
DD
geographic location (latitude)
113.97
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
activated sludge metagenome
isolation_source
activated sludge biome
local environmental context
activated sludge metagenome
metagenomic source
uncultured bacterium
organism
we used metagenomic assembly and binning methods to decipher and compare the prevalence of ARGs and their potential mobility and hosts in AS reactors treating antibiotic wastewater. To do this, we operated lab-scale sequencing batch reactors of AS process by independently adding five antibiotics of cephalexin, chloramphenicol, kanamycin, sulfamethazine and tetracycline with high concentrations. Specifically, the objectives were (1) to reveal the co-selection effects of different antibiotics for antibiotic resistance by investigating the dynamics of co-existence between ARGs, (2) to evaluate the potential mobility of ARGs by identifying the co-occurrence patterns of ARGs and MGEs, and (3) to explore the host-association of ARGs by recovering large number of metagenome-assembled genomes from AS samples.
project name
SAMN14996806,SAMN14996805
sample derived from
uncultured bacterium
scientific_name
Illumina HiSeq 4000
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: SRP262948_co1_5
title