2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22430862
SRA accession
binchicken_co158_117
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
SemiBin2;2.0.2;default
binning software
engineered
broad-scale environmental context
2018
collection date
63.33
%
completeness score
CheckM2;1.0.2;default
completeness software
0.57
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs DRR182649,DRR182650 of study DRP005178.
description
engineered
environmental medium
Japan
geographic location (country and/or sea)
35.6693
DD
geographic location (latitude)
139.4257
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
sludge metagenome
isolation_source
sludge biome
local environmental context
sludge metagenome
metagenomic source
uncultured Clostridium sp.
organism
Conductive nano-materials have been reported to accelerate methanogenesis by promoting direct interspecies electron transfer (DIET), while their effects seem to vary depending on operational conditions. The present study examined effects of magnetite nanoparticles (MNPs) on methanogenesis from acetate by soil-derived anaerobic cultures under continuous agitation. We found that MNPs accelerated methanogenesis in agitated cultures as has been observed previously for static cultures. Metabarcoding of 16S rRNA gene amplicons showed that Methanosarcina substantially increased in the presence of MNPs, while DIET-related Geobacter did not occur. Metagenomic and metatranscriptomic analyses confirmed the predominance of Methanosarcina in MPN-supplemented cultures. In addition, genes coding for acetoclastic methanogenesis but not those for hydrogenotrophic methanogenesis were abundantly expressed in the dominant Methanosarcina in the presence of MNPs. These results suggest that MNPs stimulate acetoclastic methanogenesis under continuous agitation.
project name
SAMD00175447,SAMD00175448
sample derived from
uncultured Clostridium sp.
scientific_name
Illumina NovaSeq 6000
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: binchicken_co158_117
title