2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22430631
SRA accession
ERP117304_co3_96
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
MetaBAT2;2.15;default
binning software
engineered
broad-scale environmental context
not provided
collection date
98.06
%
completeness score
CheckM2;1.0.2;default
completeness software
2.57
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3534967,ERR3534968 of study ERP117304.
description
engineered
environmental medium
not provided
geographic location (country and/or sea)
not provided
DD
geographic location (latitude)
not provided
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
biofilm metagenome
isolation_source
biofilm biome
local environmental context
biofilm metagenome
metagenomic source
uncultured Bacteroidia bacterium
organism
The establishment of epibacterial communities is fundamental to seaweed health, and fitness, in modulating ecological interactions and may also facilitate adaptation to new environments. Few recent studies have investigated the influence of abiotic factors like light, temperature as drivers of epibacterial community composition on seaweeds. Although salinity can determine bacterial abundance, growth and community composition, influence of salinity as a driver of epibacterial community composition has been rarely investigated for seaweeds and especially under long time scales. We also do not know how abiotic stressors may influence the ‘core’ bacterial species of seaweeds, if ‘core’ species exist. Following an initial (immediately after field collection, T0) sampling of epibacterial community of an invasive red seaweed Agarophyton vermicullophylum, we conducted a long term mesocosm experiment for 5 months, to examine the influence of three different salinities (low, medium and high) at two different time points (T1, T2) on the epibacterial community richness and composition of Agarophyton. Metagenomic sequencing showed that epibacterial communities changed significantly according to salinity and time points sampled. Epibacterial richness was significantly different between low and high salinities at both time points. Epibacterial richness also varied significantly among T1 and T2 within low, medium and high salinity level. Irrespective of salinity levels and time points sampled 727 taxa consistently appeared in all Agarophyton samples hinting at the presence of core bacterial species on the surface of the alga. Our results indicate that both salinity and time can be major driving forces in structuring epibacterial communities of seaweeds with respect to richness and β diversity. We highlight the necessity of conducting long term experiments allowing us to detect and understand epibacterial succession over time on seaweeds.
project name
SAMEA5789063,SAMEA5789064
sample derived from
uncultured Bacteroidia bacterium
scientific_name
Illumina HiSeq 2500
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: ERP117304_co3_96
title