2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22439499
SRA accession
binchicken_co242_126
Submitter Id
Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
MetaBAT;2.15;default
binning software
engineered
broad-scale environmental context
2017
collection date
91.01
%
completeness score
CheckM2;1.0.2;default
completeness software
1.23
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR2586215,ERR2586216 of study ERP108828.
description
engineered
environmental medium
Croatia
geographic location (country and/or sea)
45.789725
DD
geographic location (latitude)
16.08232
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
metagenome
isolation_source
metagenome
local environmental context
metagenome
metagenomic source
uncultured Bifidobacteriaceae bacterium
organism
In this work, we have examined sludge samples from a Croatian azithromycin production plant using shotgun metagenomics, to better understand the full diversity of known resistance genes, mobile genetic elements and the impact on taxonomic composition caused by extensive antibiotic selection pressures. We compare this data to sludge from a wastewater treatment plant receiving municipal sewage. We find that the sludge from pharmaceutical production harbors around three times as much resistance genes as the municipal sewage sludge, with particularly large enrichments of aminoglycoside, amphenicol and sulfonamide resistance genes. The findings highlight that antibiotic production does contribute to the development of antibiotic resistance also in European settings and indicate a large potential for co-selection of resistance genes to a variety of antibiotic classes.
project name
SAMEA4668225,SAMEA4668226
sample derived from
uncultured Bifidobacteriaceae bacterium
scientific_name
Illumina HiSeq 2500
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: binchicken_co242_126
title