2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22187349
SRA accession
ERP119705_co6_1131
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
VAMB;3.0.2;default
binning software
aquatic
broad-scale environmental context
2019-01-07
collection date
88.34
%
completeness score
CheckM2;1.0.2;default
completeness software
1.29
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3858113,ERR3858115 of study ERP119705.
description
aquatic
environmental medium
not provided
geographic location (country and/or sea)
not provided
DD
geographic location (latitude)
not provided
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
groundwater metagenome
isolation_source
groundwater biome
local environmental context
groundwater metagenome
metagenomic source
uncultured Candidatus Saccharibacteria bacterium
organism
The CRC AquaDiva (www.aquadiva.uni-jena.de) focuses on the important roles of water (Aqua) and biodiversity (Diva) for shaping the structure, properties, and functions of the subsurface, the part of the Earth's Critical Zone (CZ) that begins below the highest density of plant roots and extends down into the first aquifers. In this study, we generated short-read sequencing data for 32 groundwater samples from a well-characterized monitoring transect of the Hanich Critical Zone Exploratory (NW Thuringia, Germany) that encompasses 15 monitoring wells spread across a hillslope. Groundwater was collected from five wells (namely H14, H32, H41, H43, H51, H52) and was sequentially filtered through 0.2 uM, and 0.1 uM PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany). Filters were immediately frozen on dry ice and transported to a -80 C freezer. Library preparation and sequencing were performed with the help of the Core Facility DNA Sequencing at the Leibniz Institute on Aging (FLI, Jena, Germany). Briefly, libraries for all 32 samples were prepared using the NEB Next Ultra II DNA kit and multiplexed on four lanes on a NextSeq500 using 300 cycles for sequncing yielding 150 bp paired-end reads.
project name
SAMEA6512633,SAMEA6512635
sample derived from
uncultured Candidatus Saccharibacteria bacterium
scientific_name
NextSeq 500
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: ERP119705_co6_1131
title