2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22439956
SRA accession
binchicken_co168_317
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
SemiBin2;2.0.2;default
binning software
engineered
broad-scale environmental context
not provided
collection date
76.92
%
completeness score
CheckM2;1.0.2;default
completeness software
0.79
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3839023,SRR5581382 of study ERP119604.
description
engineered
environmental medium
not applicable
geographic location (country and/or sea)
not provided
DD
geographic location (latitude)
not provided
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
activated sludge metagenome
isolation_source
activated sludge biome
local environmental context
activated sludge metagenome
metagenomic source
uncultured Anaerolineaceae bacterium
organism
Bioelectrochemical systems (BES) exploit the interaction between microbes andelectrodes. A field of application thereof, is bioelectrochemical remediation, an effective strategy inenvironments where the absence of suitable electron acceptors limits classic bioremediationapproaches. Understanding the microbial community structure and genetic potential of anodebiofilms is of great interest to interpret the mechanisms occurring in BES. In this study, by using awhole metagenome sequencing approach, taxonomic and functional diversity patterns in theinoculum and on the anodes of three continuous-flow BES for the removal of phenol, toluene andBTEX were obtained. The genus Geobacter was highly enriched on the anodes and tworeconstructed genomes were taxonomically related to the Geobacteraceae family. To functionallycharacterize the microbial community, the genes coding for the anaerobic degradation of toluene,ethylbenzene and phenol were selected as genetic markers for the anaerobic degradation of thepollutants. The genes related with direct extracellular electron transfer (EET) were also analyzed.The inoculum carried the genetic baggage for the degradation of aromatics but lacked the capacityof EET, while anodic bacterial communities were able to pursuit both processes. Themetagenomic approach provided useful insights into the ecology and complex functions withinhydrocarbon degrading electrogenic biofilms.
project name
SAMEA6502094,SAMN07139232
sample derived from
uncultured Anaerolineaceae bacterium
scientific_name
Illumina HiSeq 2000,Illumina HiSeq 4000
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: binchicken_co168_317
title