2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22359409
SRA accession
SRP200020_co3_33
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
MetaBAT2;2.15;default
binning software
aquatic
broad-scale environmental context
2014-05-01
collection date
81.69
%
completeness score
CheckM2;1.0.2;default
completeness software
0.22
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR9166041,SRR9166048 of study SRP200020.
description
aquatic
environmental medium
Venezuela
geographic location (country and/or sea)
10.617
DD
geographic location (latitude)
-65.167
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
seawater metagenome
isolation_source
seawater biome
local environmental context
seawater metagenome
metagenomic source
uncultured Gammaproteobacteria bacterium
organism
A persistent chemoautotrophy maximum is present in many anoxic basins at the sulfidic layer’s upper boundary, but the microbiology and biogeochemistry that control this feature are poorly understood. In 13 of 31 cruises to the Cariaco Basin the particulate organic carbon pool was enriched in 13C (δ13CPOC as high as -16‰) within the oxic/sulfidic transitional layer compared to photic zone values (-23 to -26‰) and 13C enrichments persisted to depth. During these “heavy” cruises, dissolved O2 and [NO3- + NO2-] concentrations and their computed fluxes to the oxic/sulfidic interface were significantly lower than during “light” cruises. Cruises with isotopically heavy POC were more common between 2013 and 2015 when suspended particles below the photic zone tended to be nitrogen-rich compared to later cruises. Within the chemoautotrophic layer, nitrogen-rich particles (POC/PN< 10) were more likely to be 13C-enriched than nitrogen-poor particles among all cruises, implying these samples were dominated by living cells and fresh detritus rather than laterally transported or extensively decomposed biogenic debris. Dissolved inorganic carbon (DIC) assimilation during “heavy” cruises (n = 3) was faster and occurred deeper than during “light” cruises (n = 2). Metagenomics data from the chemoautotrophic layer during two cruises support prevalence of microorganisms carrying RuBisCO form II genes, which encode a carbon fixation enzyme that discriminates less against heavy isotopes than most other carbon fixation enzymes. Metatranscriptomics data confirm that form II RuBisCO genes were more highly expressed during both cruises and at depths where essential reactants coexist.
project name
SAMN11854493,SAMN11854489
sample derived from
uncultured Gammaproteobacteria bacterium
scientific_name
NextSeq 500
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: SRP200020_co3_33
title