2024-12-11
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Omic Sciences - Eurecat
INSDC center name
public
INSDC status
ERS22467301
SRA accession
E-MTAB-14650:S288C-H2 Mutant 24h-Medium2 replicate 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Cultures were incubated at 30 ˚C with shaking (220 rpm). Samples of 1.5 mL from each culture were collected at 2 h, 4 h, 6 h, 8 h, 12 h, 2 4 h, 48 h and 72 h, centrifuged for 5 minutes at 8000 g, and, after discarding the supernatant, pellets were stored at -80 ˚C until further processing.  RNA was extracted from the yeast by a lysis protocol using a combination of sonication at 20 kHZ with a 50% amplitude for 10 seconds + TRIzol® Reagent (Invitrogen, Waltham, MA, USA) following manufacturer’s instructions. RNA purification was performede using PureLink RNA Mini Kit™ (Invitrogen) following manufacturer’s instructions. RNA concentration and purity was determined using the NanoDrop™ 2000 spectophotometer (Thermo Scientific). RNA integrity was determined by capillary electrophoresis at the TapeStation using Agilent High Sensitivity RNA ScreenTape Assay (Agilent). Illumina Stranded mRNA libraries were prepared using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.
description
Mutant
genetic modification
not collected
geographic location (country and/or sea)
Medium 2
growth condition
not applicable
isolate
Saccharomyces cerevisiae S288C
organism
Saccharomyces cerevisiae S288c
organism
Saccharomyces cerevisiae S288C
scientific_name
S288C-H2 Mutant 24h-Medium2 replicate 1
title