2024-12-11
ENA first public
ERC000011
ENA-CHECKLIST
King's College London
INSDC center name
public
INSDC status
ERS20921501
SRA accession
E-MTAB-13535_4:E125_5F_GFPP_A5
Submitter Id
ArrayExpress
broker name
germ cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Experimental F1 embryos were generated by crossing Xist+/∆ (B6) dams carrying Oct4-EGFP transgene, and CAST sires, producing F1 female (XX(Xist∆)) and males (XY) that were analyzed.   Following timed natural matings, F1 embryos were harvested daily at E10.5 to E16.5. Gonads were dissected and dissociated into single-cell suspension. EGFP fluorescence was used to isolate the fluorescently marked germ cells with FACS. Viable individual germ cells and somatic cells were sorted by FACS into 96-well plates containing 4µl lysis buffer. Please see 'nucleic acid library construction protocol'. Single-cell RNA-seq libraries were generated as described in Picelli et al., 2014, Nature Protocols. Full-length RNA-seq from single cells using SMART-seq2.
description
E12.5
developmental stage
5F
embryo_id
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
gonad
organism part
Mus musculus
scientific_name
female
sex
castaneus x C57B6Nj
strain
E125_5F_GFPP_A5
title