2024-12-11
ENA first public
ERC000047
ENA-CHECKLIST
CMR
INSDC center name
public
INSDC status
ERS22452779
SRA accession
binchicken_co69_25
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaSPAdes;3.15.4;default
assembly software
coverage and kmer
binning parameters
MetaBAT2;2.15;default
binning software
engineered
broad-scale environmental context
not provided
collection date
87.52
%
completeness score
CheckM2;1.0.2;default
completeness software
1.52
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR2113683,ERR3173386 of study ERP024437.
description
engineered
environmental medium
Denmark
geographic location (country and/or sea)
not provided
DD
geographic location (latitude)
not provided
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
anaerobic digester metagenome
isolation_source
anaerobic digester biome
local environmental context
anaerobic digester metagenome
metagenomic source
uncultured Gammaproteobacteria bacterium
organism
Members of the candidate phylum Acetothermia are globally distributed and have been detected in diverse natural habitats as well as engineered systems. However, little is known about their physiology and ecological importance. In this study, an OTU belonging to Acetothermia was identified as one of the most abundant genus-level bacterial taxa in full-scale mesophilic anaerobic digesters at two Danish wastewater treatment plants. A closed genome was obtained by differential coverage binning of metagenomes and long Nanopore reads. Genome annotation and metabolic reconstruction revealed an anaerobic chemoheterotrophic lifestyle in which the bacterium obtain energy and carbon from the fermentation of peptides, amino acids, and simple sugars to acetate, formate and hydrogen. In contrast to previously studied Acetothermia, the genome did not encode a functional acetyl-CoA pathway required for acetogenesis. Fluorescent in situ hybridization probes targeting Acetothermia were designed and used in combination with confocal laser scanning microscopy and atomic force microscopy. This revealed an unusual morphology composed of a central rod cell with bipolar prosthecae similar to those observed in Caulobacter and Asticcacaulis. These prosthecae greatly expand the cell surface to volume ratio and allow for increased nutrient uptake, providing the bacteria with a competitive advantage under nutrient limited conditions.
project name
SAMEA104284473,SAMEA5366187
sample derived from
uncultured Gammaproteobacteria bacterium
scientific_name
Illumina HiSeq 2500
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: binchicken_co69_25
title