LIBEST_018886
External Id
SAMN00177610
External Id
M. S. Swaminathan Research Foundation
INSDC center name
2006-01-09T00:00:00Z
INSDC first public
2024-10-23T21:06:07.992Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS21410696
SRA accession
Prosopis juliflora seeds collected from Kudankulam,Tamil Nadu, India were germinated and grown on a mixture of soil and sand (1:1) under the green house conditions with daily watering. After two months, watering was withheld for 25 days. After 25 days poly(A+) mRNA from the leaf tissue was purified and cDNA was prepared according to kit instructions ( Creator SMART cDNA Library Construction Kit, BD Biosciences Cat. No. 634903). The cDNA was size-fractionated over a SizeSep-400 spun column (Amersham-Pharmacia Biotech, Piscataway, N.J. Cat No. 27-5105-01 ) and directionally cloned in the 5 Sfi I A and 3 Sfi I B sites of p DNR_LIB vector (Clontech,Cat NO. 631611). The ESTs were sequenced from the 5' end using a primer specially designed near the 5 end of the cDNA insert in an Automated Sequencer (ABI310, Applied Biosystems) and submitted after editing to remove the vector and adapter sequences. BLAST search in BLASTX (Non-redundant) was carried out on 7th August, 2004 using default parameters and the results reported under putative identification/comment
description
Two month old plant
dev-stage
Neltuma juliflora
http://purl.obolibrary.org/obo/NCBITaxon_3128859
organism
hermaphrodite
sex
Kudankulam
strain
leaf
http://purl.obolibrary.org/obo/PO_0025034
tissue-type
Prosopis juliflora leaf cDNA library
title