SAMN00163283
External Id
LIBEST_008758
External Id
National University of Ireland, Galway
INSDC center name
2001-05-31T00:00:00Z
INSDC first public
2024-05-06T21:08:40.276Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS19586406
SRA accession
A Pacific oyster gill cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5_g of mRNA and the library was constructed by directional cloning EcoRI-XhoI based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum. The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E.coli cell line XL1-Blue MRF'.
description
Adult
http://www.ebi.ac.uk/efo/EFO_0001272
dev-stage
Magallana gigas
http://purl.obolibrary.org/obo/NCBITaxon_29159
organism
EcoRI
re_1
XhoI
re_2
Gill
http://purl.obolibrary.org/obo/UBERON_0002535
tissue-type
Pacific oyster Lambda Zap Express gill cDNA library
title
Phage
v_type
Lambda ZAP Express
vector