LIBEST_020422
External Id
SAMN00154366
External Id
Mount Desert Island Biological Laboratory
INSDC center name
2006-09-20T00:00:00Z
INSDC first public
2024-10-07T21:09:35.247Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS21120383
SRA accession
Egg cases of the Little Skate (Leucoraja erinacea) were purchased from the Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A. Embryos were staged according to a scheme developed for Scyliorhinus canicula, the Lesser Spotted Dogfish (J. exp. Zool. 1993. 267:318-336). Using TRIzol reagent, total tissue RNAs were extracted from four complete embryos, 1 at stage 19, 2 at stage 20, and 1 at stage 25 during which time the pharyngeal pouches develop and open, and the first gill buds appear. The primary cDNA library was constructed from mRNA, directionally cloned in DH10Bª (T1 and T5 phage resistant) and normalized by Invitrogen. The average insert size was 1.9 kb. Inserts were single-pass sequenced from the 5' end by the University of Washington High-Throughput Genomics Unit, Department of Genome Sciences, Seattle, Washington. Traces were trimmed and prepared at the Mount Desert Island Biological Laboratory, Maine, for submission to dbEST (GenBank) using trace2dbest software (Parkinson, Anthony, and Blaxter, unpublished). This expressed sequence tag project was supported by NIH Grant Number 1 RO1 019732-01 from the National Center for Research Resources (D.W. Barnes, PI), the Woodcock Foundation (D.W. Barnes and C.J. Bayne, PI) and NIH grant P20-RR-1 for Research Resources (P.H. Hand, PI).
description
19, 20 and 25
dev-stage
Whole embryo
http://purl.obolibrary.org/obo/UBERON_0000922
organ
Leucoraja erinaceus
http://purl.obolibrary.org/obo/NCBITaxon_7782
organism
Little skate (Leucoraja erinacea) embryo tissues; 5' sequences of ESTs
title
pCMV-SPORT6.1
vector