LIBEST_024499
External Id
SAMN00167490
External Id
Forestry and Forest Products Research Institute
INSDC center name
2009-10-01T00:00:00Z
INSDC first public
2024-10-08T21:05:21.931Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS21137448
SRA accession
To obtain mixed-stage nematodes, the nematodes were cultured on fungi (Botrytis cinerea grown on autoclaved barley) for 3 weeks at 25C and then extracted for 3h at 25C using the Baermann funnel technique. Poly(A)+ RNA was extracted using Sepasol (Nakalai) and subsequently FastTrack MAG micro mRNA Isolation Kits (Invitrogen). The cDNA was synthesized using the SMART technology (Clontech) and cloned into pSPORT1 using an Adaptor (SalI-SmaI, TAKARA). The plasmid construct was transformed into E. coli DH5a by electroporation.
description
mixed
http://www.ebi.ac.uk/efo/EFO_0001271
dev-stage
Aphelenchoides avenae
http://purl.obolibrary.org/obo/NCBITaxon_70226
organism
SalI
re_1
NotI
re_2
Aphelenchus avenae mixed-stage library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
PSPORT1
vector