LIBEST_024833
External Id
SAMN00167835
External Id
National University of Ireland Maynooth
INSDC center name
2009-07-06T00:00:00Z
INSDC first public
2024-10-08T21:05:21.982Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS21137446
SRA accession
Total RNA from mixed stage desiccated nematodes (85% RH for 24 hours) was converted to 5'-oligo-capped cDNA using the GeneRacerTM Kit (Invitrogen). PCR was carried out on the 5'-oligo-capped cDNA with the GeneRacerTM 5' and 3' primers. The products of this reaction were cloned into the pCR2.1 TOPO vector using a TOPO-TA Cloning kit (Invitrogen).
description
Mixed stage population
dev-stage
E. coli TOP10
lab-host
Aphelenchoides avenae
http://purl.obolibrary.org/obo/NCBITaxon_70226
organism
Aphelenchus avenae desiccated at 85% RH for 24 hours
title
pCR2.1
vector