LIBEST_015941
External Id
SAMN00175165
External Id
Washington University School of Medicine
INSDC center name
2004-06-29T00:00:00Z
INSDC first public
2024-12-04T21:05:42.175Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS22578758
SRA accession
A pool of mRNA was primed with an anchored oligo-dT adaptor with a Not I site for 1st strand synthesis. Double stranded cDNA was ligated to Sal-Adaptors and cut with NotI. After size selection (without radioactivity) cDNA was ligated into pSport with SalI/NotI termini. Transformation was in DH10b Electromax T1 phage resistant cells. Plating was on 2YT/Carbenicillin Agar and 14x384 cells were blue/white selected automatically picked into 2YT/HMFM/Carbenicillin (HMFM is a freezing additive).
description
DH10B
lab-host
Klyxum echinatum
http://purl.obolibrary.org/obo/NCBITaxon_3283270
organism
Not I
re_1
Sal I
re_2
HyEch JUMY T1
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pSPORT1
vector