LIBEST_026811
External Id
SAMN00169900
External Id
DOE Joint Genome Institute
INSDC center name
2010-12-02T00:00:00Z
INSDC first public
2024-05-06T21:08:40.799Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS19586436
SRA accession
The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).
description
larval
dev-stage
DH10B
lab-host
Magallana gigas
http://purl.obolibrary.org/obo/NCBITaxon_29159
organism
SalI
re_1
NotI
re_2
Taylor Oyster Hatchery - Manahan Lab 2005
strain
CBSN Crassostrea gigas 2-12 hrs, 24 degrees Crassostrea gigas (L)
title
pCMV-SPORT6
vector