2024-12-13
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112232474
External Id
Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
INSDC center name
2022-12-13T16:26:46Z
INSDC last update
public
INSDC status
ERS14343369
SRA accession
E-MTAB-12462:GAP_2
Submitter Id
ArrayExpress
broker name
TLBR2
cell_line
human
common name
Protocols: Cells were collected after 24 hours from lncMAAT silencing. Cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 20U/ml IL2. TLBR-2 cells were transfected with 50nM Antisense LNA GapmeRs concentration. Antisense LNA GapmeR transfections was performed using the Cell Line Nucleofector Kit SF and Amaxa 4D Nucleofector (program DS-130). 24 hours after transfection, cells were harvested for RNA extraction. Antisense LNA GapmeR Negative Control (Cat. No. / ID: 33951, Qiagen) was used as negative control. Total RNA from cells was extracted by TRIzol (Thermo Fisher Scientific) according to the manufacture’s instructions. Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.
description
adult
developmental stage
anaplastic large cell lymphoma
disease
wild type genotype
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
GAP_2
title