SAMN00172556
External Id
LIBEST_013043
External Id
Cooperative Research Centre for Tropical Plant Protection and Plant Cell Biology Group, Research School of Biological Sciences, Australian National University
INSDC center name
2004-01-01T00:00:00Z
INSDC first public
2024-01-11T21:07:24.995Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS17748265
SRA accession
The cDNA library was constructed in lambda Uni-ZAP XR vector from poly(A) RNA isolated from zoospores released from 1-2 week old mycelium and centrifuged at 2000g for 3 min. The primary library was amplified once in XL1-Blue MRF' strain. A portion of the amplified library was mass-excised using ExAssist helper phage for conversion into pBluescript phagemid and plated on SOLR strain. Plasmids were isolated and sequenced using T3 primer corresponding to the 5' end of the mRNA.
description
zoospore
dev-stage
E. coli strain SOLR
lab-host
Phytophthora nicotianae
http://purl.obolibrary.org/obo/NCBITaxon_4792
organism
Eco RI
re_1
Xho I
re_2
H1111 (ATCC MYA-141)
strain
P. nicotianae zoospore
title
pBluescript
vector