SAMN00153082
External Id
LIBEST_021358
External Id
IFREMER-CNRS-Universite de Montpellier 2
INSDC center name
2007-06-26T00:00:00Z
INSDC first public
2024-05-06T21:08:40.088Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS19586398
SRA accession
Subtracted libraries were constructed with the PCR-select cDNA subtraction kit (Clontech). For subtractive library construction, mRNAs were extracted from a pool of four million embryos (morula, blastula, gastrula), from a pool of four million D-hinged larvae and from a pool of 20 spats. The cDNA fragments obtained were cloned into pCRR 4-TOPO cloning vector using TOPO TA cloning kit (Invitrogen) and transformed into Escherichia coli TOP10 One Shot Chemically Competent cells (Invitrogen).
description
Magallana gigas
http://purl.obolibrary.org/obo/NCBITaxon_29159
organism
Crassostrea gigas early developmental stages, D-hinged larvae and spat subtracted libraries
title
pCRR 4-TOPO cloning vector from TOPO TA cloning kit (Invitrogen)
vector