characteristics.text: 2024-12-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115057854
characteristics.characteristic: External Id
characteristics.text: Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA-CSIC)
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-22T14:22:35Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17598350
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13675:Low_H 3
characteristics.characteristic: Submitter Id
characteristics.text: 4
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Protocols: Plant material was harvested and frozen into liquid nitrogen before or after 30min of mite infestation. Arabidopsis thaliana Col-0 accession was used. Seeds were planted and incubated 5 days at 4ºC in peat moss and vermiculite (3:2 V/V). Plants were then grown in growth chambers (Sanyo MLR-350-H) under control conditions (23ºC±1ºC, >70% relative humidity and a 16 h/8 h day/night photoperiod) or at different temperatures and soil humidity conditions (27ºC and/or a quarter of soil humidity of control conditions). A colony of Tetranychus urticae, London strain (Acari: Tetranychidae) provided by Dr. Miodrag Grbic (UWO, Canada), was reared on beans (Phaseolus vulgaris) and maintained in growth chambers (Sanyo MLR-350-H) at 25ºC±1ºC, >70% relative humidity and a 16h/8h day/night photoperiod. Mites were synchronized and four-week-old plants were infested with 20 T. urticae female adults per plant. They were carefully transferred with a brush to the leaf surface. Total RNA was isolated from frozen rosettes and purified by using the RNeasy Qiagen Mini Plant Kit (74904 Qiagen), including the on-column DNA I (79254, Qiagen) digestion recommended by the manufacturer. RNA amount and quality were tested in a Nanodrop ND-1000. Using poly-T oligo-attached magnetic beads, mRNAs were purified from the total RNA. Then, the mRNAs were fragmented and cDNA was synthesized using random hexamer-primers, DNA polymerase I and RNase H. The double-stranded cDNAs were purified with magnetic beads and ligated to adaptors for Illumina sequencing. The quality and quantity of the library was verified using an Agilent 2100 Bioanalyzer and an ABI StepOnePlus Real-Time PCR system, respectively.
characteristics.characteristic: description
characteristics.text: Columbia-0
characteristics.characteristic: ecotype
characteristics.text: Low humidity
characteristics.characteristic: environmental stress
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: rosette
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: -
characteristics.characteristic: stimulus
characteristics.text: Low_H 3
characteristics.characteristic: title