2024-12-16
ENA first public
ERC000011
ENA-CHECKLIST
PDRA
INSDC center name
public
INSDC status
ERS22496221
SRA accession
E-MTAB-14657:Prepupa_3
Submitter Id
ArrayExpress
broker name
not collected
collection date
jewel wasp
common name
Protocols: For RNA samples 400 embryos, 30 larvae, 10 prepupa, 10 pupa and 10 adults were used per replicate (3 replicates for each stage). Samples were extracted using the Tri-Reagent (Sigma Aldrich) protocol, with adjustments for TURBO DNase TM treatment and removal outlined in the paper. RNA concentration was measured using the QubitTM RNA BR Assay Kit (ThermoFisher) and the integrity of RNA was assessed on a Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent). RNA samples collected in the studies were converted to cDNA by BGI genomics (Hong Kong). Library preparation of these cDNA fragments was also prepared by BGI Genomics (Hong Kong).
description
prepupa
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Nasonia vitripennis
organism
Nasonia vitripennis
organism
whole organism
organism part
Nasonia vitripennis
scientific_name
female
sex
Leicester
strain
Prepupa_3
title