SAMN38285591
External Id
INIA-CSIC
INSDC center name
2024-12-15T00:50:23.980Z
INSDC first public
2024-12-15T00:50:23.980Z
INSDC last update
SRS19573675
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS19573675
SRA accession
36Cshoot 36C root
air_temp_regm
2019
collection_date
NA
cultivar
For the analyses of microbiota abundances, tomato seeds were germinated at 22C during 1 week in sterile vermiculite. Afterwards, seedlings were transferred to pots (1 per pot) filled with 3 liter of natural soil obtained from coordinates 4024'21.6"N 349'58.1"W that were mixed with 1 part of clean river sand. These plants were cultivated for 3 weeks at three different temperatures 22C (22C shoot and root; 22SR), 36C (36C in root and shoot, 36SR) and 36TGRZ (36C in shoot and gradient from 36 to 20C in root) and irrigated with 150 ml of sterile water twice a week. We took 6 samples of the initial soil and maintained them at -20C. After 3 weeks, we took 6 samples of soil corresponding to rhizosphere and 6 samples of roots that were extensively washed to eliminate soil/microorganism traces) and freeze to -20C. Soil and roots were taken from the same area in the pot, corresponding to an intermedium part of the root system.
description
NA
ecotype
oaks land
env_broad_scale
forest
env_local_scale
clay
env_medium
Spain: Madrid,Pozuelo de Alarcon
geo_loc_name
tomato
host
clay was mixed with 1 part of clean river sand. These plants were cultivated for 3 weeks at three different temperatures 22C (22C shoot and root; 22SR), 36C (36C in root and shoot, 36SR) and 36TGRZ (36C in shoot and gradient from 36 to 20C in root) and irrigated with 150 ml of sterile water twice a week.
isol_growth_condt
a39
isolate
rhizosphere
isolation_source
40.24 N 3.49 W
lat_lon
rhizosphere metagenome
http://purl.obolibrary.org/obo/NCBITaxon_939928
organism
NA
strain
Metagenome or environmental sample from rhizosphere metagenome
title
irrigated with 150 ml of sterile water twice a week.
watering_regm