characteristics.text: SAMN02437424
characteristics.characteristic: External Id
characteristics.text: Genotypic Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2013-12-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-27T02:03:51.772Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS21173104
characteristics.characteristic: SRA accession
characteristics.text: Apis mellifera intermissa (Buttel-Reepen, 1906) is the native honeybee subspecies of Algeria. A.m.intermissa occurs in Tunisia, Algeria and Morocco, between the Atlas and the Mediterranean and Atlantic coasts (Ruttner, 1988), in an area of more than 2500 km long. Intermissa indicates the position through this bee races between tropical Africa and European breeds (Peyvel, 1994). The settlement area of the Tellian extends from Tunisia to Morocco. ~5 microgram of genomic DNA was made up to 50 ul with nuclease free water (Ambion) and sonicated using covaris to obtain desired fragment length ranging between 150 to 600 bp. The resulting fragmented DNA was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). The size distribution was checked by running an aliquot of the sample on Agilent High Sensitivity Bioanalyzer Chip. Subsequently libraries for whole genome sequencing were constructed according to the TruSeq DNA library protocol outlined in “TruSeq DNA Sample preparation guide”. DNA was subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, and add a single nucleotide A overhang and ligate adaptors (Illumina’s TruSeq DNA sample preparation kit). Sample cleanup was done using Ampure SPRI beads. After ligation, ~300 - 500 bp was size selected on a 2% agarose gel and cleaned using MinElute column (QIAGEN). PCR amplification was done and cleaned up using Ampure SPRI beads. The prepared libraries were quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip .
characteristics.characteristic: description
characteristics.text: Algeria
characteristics.characteristic: geo loc name
characteristics.text: Apis mellifera intermissa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_346610
characteristics.characteristic: organism
characteristics.text: Apis mellifera intermissa
characteristics.characteristic: strain
characteristics.text: intermissa
characteristics.characteristic: sub species
characteristics.text: Leg tissue
characteristics.characteristic: tissue type
characteristics.text: General Sample for Apis mellifera intermissa
characteristics.characteristic: title