2024-12-19
ENA first public
ERC000021
ENA-CHECKLIST
UniBremen
INSDC center name
public
INSDC status
ERS22115429
SRA accession
tempSIP_22
Submitter Id
0.045
L
amount or size of sample collected
shallow marine sediment [ENVO:03000034] | polar biom [ENVO:01000339] | glacial retreat [ENVO:01001656] | coast [ENVO:01000687]
broad-scale environmental context
acetate, lepidocrocite
chem_administration
2019-01-23
collection date
0 - 0.29
m
depth
Potter Cove sediment slurry SIP incubation with unlabeled acetate and lepidocrocite at 15 degree Celsius, RNA extract used for fractionation, sediment from station 13, core STA13.04, Potter Cove, Antarctica; parameters geographic location, depth and collection date  refer to sediment used to prepare the slurry; experiment set up in biological triplicates and pooled for extraction
description
-15
m
elevation
microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
environmental medium
different substrate additions | RNA stable isotope probing | exposure temperature [EFO:0000718] | response to heat [GO:0009408] | labelling [EFO:0000562]
experimental factor
Antarctica
geographic location (country and/or sea)
-62.2253889
DD
geographic location (latitude)
-58.6411667
DD
geographic location (longitude)
King George Island/Isla 25 de Mayo, Potter Cove
geographic location (region and locality)
10.1007/978-1-4939-9721-3_15
internal_standard
13C
isotope
175
isotopolog
single
isotopolog_approach
0.011
isotopolog_atom_frac
1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1/i1+1,2+1;
isotopolog_atom_pos
30.026
ppm
isotopolog_dose
T264:00
hour
isotopolog_incu_time
natural abundance
isotopolog_label
anaerobic sediment [ENVO:00002045]
local environmental context
marine sediment metagenome
organism
anaerobic
oxygenation status of sample
Bacterial 16S rRNA community in acetate degrading stable isotope probing slurry incubation experiment across temperature range with sediment of Potter Cove
project name
anaerobe
relationship to oxygen
syringe, needle
sample collection device
slurry sampling in anoxic manner
sample collection method
slurry triplicates were pooled and sample was frozen at -80 degrees C until RNA extraction
sample material processing
45
mL
sample volume or weight for DNA extraction
marine sediment metagenome
scientific_name
10.1007/978-1-4939-9721-3_15
sip_method
slurry prepared in ratio 1:6 of sediment Potter Cove core STA13.04 and sulfate-free artificial sea water
source material identifiers
Potter Cove sediment slurry SIP incubation - 12C Acetate.Lepidocrocite at 15 degrees C - RNA extract for fractionation
title