LIBEST_028480
External Id
SAMN02946960
External Id
National Research Center on Plant Biotechnology
INSDC center name
2015-07-31T00:00:00Z
INSDC first public
2024-04-24T21:09:24.229Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS22629796
SRA accession
PPL (Pusa Purple Long)
cultivar
SSH library prepared from 50 dpa fruit of eggplant (Solanum melongena) cv. PPL. RNA was isolated from 50 dpa fruit using Spectrum Plant total RNA kit of Sigma (Cat.No. STRN50-1KT). SSH was carried against 50 dpa fruit of eggplant cv. KKM1 as driver to detect the genes especifically expresses in PPL cultivar during 50 dpa stage. Clontech PCR -Select cDNA Subtraction kit (cat. No.637401) was used to make the libraries. All the sequences were generated as single pass reads, using M13 reverse primer. Sequences submitted are trimmed from low quality sequences and pGEMT-Easy vector backbone.
description
50 dpa
http://purl.obolibrary.org/obo/PO_0009001
dev-stage
Fruit
http://purl.obolibrary.org/obo/PO_0009001
organ
Solanum virginianum
http://purl.obolibrary.org/obo/NCBITaxon_223891
organism
Library from 50 dpa fruit of eggplant cv. PPL
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pGEMT-Easy
vector