SAMD00027648
External Id
Tropical Biosphere Research Center Genomics Unit, Center of Molecular Biosciences
INSDC center name
2015-04-15T00:00:00Z
INSDC first public
2024-06-02T01:21:23.303Z
INSDC last update
DRS032512
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS032512
SRA accession
Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 micro mol m-2 s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 deg C for over six days (i.e. temperature was lowered from 30 deg C to 12 deg C in 6 deg C daily increments over the first four days and then elevated again to 30 deg C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA).Samples were collected during the day at time points of 0.5 h (on Days 1 and 2) and 6 h (on Day -3 to 2), for a total of seven time points. A leaf per sapling was harvested at each sample time point.
description
Ficus septica
http://purl.obolibrary.org/obo/NCBITaxon_100573
organism
Fs_IS738
sample name
leaf
http://purl.obolibrary.org/obo/PO_0025034
tissue type
Ficus septica leaf sample IS738 (Day -2 6h) for whole transcriptome shotgun sequencing
title