characteristics.text: SAMN04450423
characteristics.characteristic: External Id
characteristics.text: McDonnell Genome Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2016-02-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-11-22T21:05:42.252Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Invertebrate
characteristics.characteristic: NCBI submission model
characteristics.text: Invertebrate.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS22449506
characteristics.characteristic: SRA accession
characteristics.text: 1989
characteristics.characteristic: collection date
characteristics.text: Fasciolopsiasis is caused by the infection of a giant intestinal fluke, Fasciolopsis buski . It is classified in the Fasciolidae, and with the the liver fluke genus Fasciola with which it shares a similar developmental cycle and morphology. F. buski infection is largely con###ned to Asian countries, namely southern China, India, Bangladesh, Thailand, Malaysia, Borneo, Sumatra and Myanmar. Prevalence of infection can be high within endemic areas. The parasite attaches to the intestinal wall of the definitive hosts (human and pig), after infection through consumption of viable metacercariae attached to stems and roots of water plants. F. buski metacercariae excyst in the duodenum and the juvenile worms attach to the duodenal and jejunal wall. The larvae develop to mature adults within three## months and produce large numbers of eggs (an estimated 10000-25000 eggs per worm per day). Eggs are large, yellowish in colour, and operculate. If the eggs reach fresh water sources, further development and embryonation occur over 3###7 weeks, then miracidia hatch and penetrate snail intermediate hosts in the family Planorbidae, especially in the genus Segmentina, Hippeutis and Gyraulus. After transformation and asexual multiplication as sporocysts, rediae and cercariae, free-swimming cercariae released from the snails attach and encyst on any freshwater plant surface, and subsequently are consumed by the definitive host. The attachment of parasites on the duodenal or jejunal wall can cause low grading eosinophilia (<35%), where mechanical injury and inflammation lead to ulcer formation. These ulcers sometimes bleed due to capillary damage then may lead to petechial bleeding and the formation of intestinal abscesses. Sequencing the genome of F. buski will enable understanding the parasite, infectious process and host-parasite interactions at a molecular level. DNA material for the genome sequencing was provided by Dr. Thanh Hoa Le (imibtvn@gmail.com) at Institute of Biotechnology, Vietnam and Paul Brindley (pbrindley@email.gwu.edu) at George Washington University, Washington DC. The stain of Fasciolopsis buski Looss 1899 has been defined as the HT strain (HT is abbreviation of Ha Tay town, just 20 km from Hanoi where this sample was isolated) collected in Ha Tay town, near Hanoi, Vietnam.
characteristics.characteristic: description
characteristics.text: Viet Nam
characteristics.characteristic: geo loc name
characteristics.text: pig
characteristics.characteristic: host
characteristics.text: intestine
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0000160
characteristics.characteristic: isolation source
characteristics.text: Fasciolopsis buskii
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_27845
characteristics.characteristic: organism
characteristics.text: HT
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002200
characteristics.characteristic: strain
characteristics.text: Invertebrate sample from Fasciolopsis buskii
characteristics.characteristic: title