characteristics.text: 2024-12-20
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Biology, University of Fribourg.
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21132228
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14522:CWC85_01_let418(s1617)
characteristics.characteristic: Submitter Id
characteristics.text: 50 hours post-L1 stage
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: At least 1000 animals from wild-type and let-418(s1617) strains were synchronized and collected as young adult hermaphrodites 50 hours post-larval hatching after hypochlorite treatment, at 20°C (Stiernagle, T. 2006)) Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA) and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad IA, USA) according to the manufacturer's instructions cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) by the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/)
characteristics.characteristic: description
characteristics.text: newly molted young adult hermaphrodite
characteristics.characteristic: developmental stage
characteristics.text: let418(s1617)
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: CWC85
characteristics.characteristic: strain
characteristics.text: CWC85_01_let418(s1617)
characteristics.characteristic: title