characteristics.text: SAMD00021020
characteristics.characteristic: External Id
characteristics.text: Tokyo Medical and Dental University Medical Research Institute Gene Expression
characteristics.characteristic: INSDC center name
characteristics.text: 2016-01-29T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T01:47:56.297Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS023187
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS023187
characteristics.characteristic: SRA accession
characteristics.text: The worm strain used was N2. This strain is wild type. Total RNAs were extracted from synchronized L1 worms by using RNeasy Midi kit (Qiagen). Poly (A)+ RNAs were repeatedly purified with FastTrack MAG (Invitrogen), fragmented in 1Â~ Fragmentation buffer (Ambion) and reverse transcribed using random hexamers and Primescript II (Takara). Second strand cDNAs were synthesized by using RNase H (Invitrogen) and DNA polymerase I (Invitrogen). Deep sequencing was performed by utilizing Genome Analyzer II (Illumina) following standard protocols.
characteristics.characteristic: description
characteristics.text: L1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001084
characteristics.characteristic: dev stage
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: N2 L1
characteristics.characteristic: sample name
characteristics.text: N2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004028
characteristics.characteristic: strain
characteristics.text: N2 L1 [RNAseq]
characteristics.characteristic: title