characteristics.text: 2024-12-21
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Suzhou Ninth People's Hospital
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22467316
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14651:HCT116_4.5_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell_line
characteristics.text: colorectal cancer cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: For cell digestion, medium was removed, and cells with 80% density were simply washed by PBS twice. 1 mL 0.25% trypsin-EDTA (Thermo Fisher Scientific) was added into 10 cm dish, and uniformly dispersed for 0.5 min at 37 oC, then quenched immediately by 4 mL regular medium. The suspended cells were harvested after centrifugation of 5 mL supernatant. 1 mL trypsin was added again to digest the rest of cells for 2 min at 37 oC, then harvested the suspended cells as same procedure as above. The third round of 1 mL trypsin was added to digest the rest of cells for 4 min at 37 oC. These three batches of cells were used for the consequent experiments. polysome-associated RNA was extracted from at least 5 × 106 cells using the FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, China) according to the manufacturer’s protocol.  Isolation of mRNA was performed using the NEBNext PolyA mRNA Magnetic Isolation Module (New England Biolabs, USA) and the mRNA was further used with the NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) for sequencing library preparation. The libraries were quantified with a Qubit 4.0 (Thermo Fisher Scientific), and the distribution of fragment sizes was determined using an Agilent Bioanalyzer 2100 (Agilent, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal neoplasm
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCT116_4.5_2
characteristics.characteristic: title