SAMD00046171
External Id
Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences , University of Tokyo
INSDC center name
2018-08-09T00:00:00Z
INSDC first public
2024-06-02T02:45:25.333Z
INSDC last update
DRS073579
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS073579
SRA accession
PIK64 (deltaHPP12_488::aphA). Stationary phase. strand-specific mRNA-seq.This strain was grown in Brucella both containing 10% FBS up to stationary phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using SureSelect - Strand specific RNA library kit (Agilent Technologies). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
description
Helicobacter pylori P12
http://purl.obolibrary.org/obo/NCBITaxon_570508
organism
PIK64_stat_SS
sample name
PIK64_stat_SS
title