SAMD00046176
External Id
Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences , University of Tokyo
INSDC center name
2018-08-09T00:00:00Z
INSDC first public
2024-06-02T02:45:24.220Z
INSDC last update
DRS073584
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS073584
SRA accession
PIK69 (delta(HPP12_510-HPP12_511)::aphA). Exponential phase. strand-specific mRNA-seq.This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using SureSelect - Strand specific RNA library kit (Agilent Technologies). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
description
Helicobacter pylori P12
http://purl.obolibrary.org/obo/NCBITaxon_570508
organism
PIK69_exp_SS
sample name
PIK69_exp_SS
title