2024-12-26
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114321693
External Id
GTTCATTAAACT
Forward barcode
Royal Netherlands Institute for Sea Research (NIOZ)
INSDC center name
2023-09-01T13:58:01Z
INSDC last update
public
INSDC status
GAGCGCCGAACA
Reverse barcode
ERS16310805
SRA accession
NIOZ310.217.208
Submitter Id
515F_Golay217 and 926RBC_Golay208
barcodenames
2010-04
collection date
St3 Schiermoniekoog Microbial mat
description
GTGYCAGCMGCCGCGGTAA
forwardprimersequence
Netherlands
geographic location (country and/or sea)
29' 28.3626
DD
geographic location (latitude)
6� 8' 20.9646
DD
geographic location (longitude)
Dneasy Powersoil kit
nucleic acid extraction
microbial mat metagenome
organism
initial denaturation at 98 C for 30 s; 30 cycles of 98 C for 30 s, 50 C for 15 s, and 72 C for 30 s; final extension at 72 C for 5 minutes
pcr conditions
Forward Primer (515F) GTGYCAGCMGCCGCGGTAA Reverse Primer (926R) CCGYCAATTYMTTTRAGTTT
pcr primers
HglT primers
project name
AmpureBeads
purificationmethod
CCGYCAATTYMTTTRAGTTT
reverseprimersequence
microbial mat metagenome
scientific_name
Illumina MiSeq
sequencing method
16S rRNA
target gene
V4V5
target subfragment
600bp400bp
targetedampliconsize
Mmat_Schier_st3_Apr10
title