characteristics.text: SAMD00023371
characteristics.characteristic: External Id
characteristics.text: Kanazawa University Division of Molecular Cell Signaling, Cancer Resarch Institute Yoshioka Lab
characteristics.characteristic: INSDC center name
characteristics.text: 2019-01-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T01:51:38.430Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS085820
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS085820
characteristics.characteristic: SRA accession
characteristics.text: 4 weeks
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000034
characteristics.characteristic: age
characteristics.text: Cerebellums (50-90 mg) from controls mice aged 4 weeks were used to extract total RNAs. RNA was isolated with Sepasol-RNA I Super G (Nacalai Tesque, Kyoto, Japan) according to the manufacturer's instructions. All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).
characteristics.characteristic: description
characteristics.text: floxed Jsap1:Jlp without GluRdelta2-Cre
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: phenotype
characteristics.text: PC_cont_4w RNAseq
characteristics.characteristic: sample name
characteristics.text: Jsap1flox/flox:Jlpflox/flox (backcrossed for ten generations onto a C57BL/6 background)
characteristics.characteristic: strain
characteristics.text: cerebellum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UBERON_0002037
characteristics.characteristic: tissue type
characteristics.text: PC_cont_4w RNAseq
characteristics.characteristic: title