characteristics.text: SAMD00027917
characteristics.characteristic: External Id
characteristics.text: Tokyo Medical and Dental University Medical Research Institute Gene Expression
characteristics.characteristic: INSDC center name
characteristics.text: 2019-03-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T01:55:29.537Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS092273
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS092273
characteristics.characteristic: SRA accession
characteristics.text: Hidehito KUROYANAGI
characteristics.characteristic: biomaterial provider
characteristics.text: 2014-03-18
characteristics.characteristic: collection date
characteristics.text: The worm strain used was N2. This is a standard wild-type worm strain. Embryos were obtained by bleaching synchronized gravid adults. The embryos were incubated for 18 hours at 20 degree Celsius in M9 buffer. The synchronized L1 worms were then fed with E. coli strain OP50 suspended in S-complete medium for 3 hours at 20 degree Celsius. The L1 worms were cleaned by washing with the M9 buffer and total homogenate was prepared by grinding the worms in liquid nitrogen. Cytoplasmic fraction (clear supernatant) was prepared by centrifuging the total homogenate. Total RNAs were extracted from the cytoplasmic fraction by using Sepasol I Super (nacalai tesque) and then purified by using RNeasy Plus Mini kit plus DNase I (Qiagen).
characteristics.characteristic: description
characteristics.text: L1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0001084
characteristics.characteristic: dev stage
characteristics.text: wild type
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0005168
characteristics.characteristic: genotype
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: 10_N2_L1_cytoplasm
characteristics.characteristic: sample name
characteristics.text: hermaphrodite
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001340
characteristics.characteristic: sex
characteristics.text: N2
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0004028
characteristics.characteristic: strain
characteristics.text: N2 L1 cytoplasmic fraction [RNAseq]
characteristics.characteristic: title