2024-12-29
ENA first public
ERC000011
ENA-CHECKLIST
STANFORD UNIVERSITY
INSDC center name
public
INSDC status
ERS22569228
SRA accession
E-MTAB-14693:DENV-2_16681_Hap1-adapted
Submitter Id
ArrayExpress
broker name
HuH-7.5.1
cell_line
hepatic cell
cell_type
not collected
collection date
human
common name
Protocols: >1000-fold coverage of mutagenized cells was infected with DENV2 at MOI 0.1. Resistant cell colonies were harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). A first round of PCR was used to amplify the guide RNA sequences encoded in the gDNA, followed by a second round of PCR to add the barcodes/adapters for amplicon sequencing. 2% agarose gels and a QIAquick gel extraction kit were used to purify the amplicons.
description
adult
developmental stage
Adult hepatocellular carcinoma
disease
lentiCas9-Blast; lentiGuide-Puro
genotype
not collected
geographic location (country and/or sea)
DENV2_16681_Hap1-adapted
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
Homo sapiens
scientific_name
DENV-2_16681_Hap1-adapted
title