2024-12-29
ENA first public
ERC000011
ENA-CHECKLIST
STANFORD UNIVERSITY
INSDC center name
public
INSDC status
ERS22569243
SRA accession
E-MTAB-14694:NITD-688_1
Submitter Id
ArrayExpress
broker name
HuH-7.5.1
cell_line
hepatic cell
cell_type
not collected
collection date
human
common name
Protocols: Huh7.5.1 cells were seeded in 12-well plates, with three replicates for each treatment. Cells were incubated for 48 h with LY-411575, JNJ-1802, NITD-688, and NITD-008 at their respective EC90 concentrations against DENV: 10 nM, 0.1 nM, 7 nM, and 1.3 μM. Cells treated with DMSO served as controls. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, #74106). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.
description
adult
developmental stage
Adult hepatocellular carcinoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
Homo sapiens
scientific_name
NITD-688_1
title