SAMD00026085
External Id
Kyoto Prefectural University
INSDC center name
2019-09-15T00:00:00Z
INSDC first public
2024-06-02T01:53:00.067Z
INSDC last update
DRS107611
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS107611
SRA accession
Mami Nomura, Ken-ichiro Ishida, Graduate School of Life and Environmental Science, University of Tsukuba
biomaterial provider
2009-10-09
collection date
P. micropora MYN1 strain was cultured under 14 hours light (30-40 microE m-2s-1) / 10 hours dark photoperiodic cycle at 25 degree C in the modified Waris-H+Si medium. The cells at the time points of 0, 4, 8, 12, 16, and 20 hours since the start of light-phase were harvested by low-speed centrifugation (500 x g, 2 minutes, 4 degree C), and then they were passed through 20 micrometer mesh-filter (CellTrics, Sysmex Partec GmbH, Gorlitz, Germany) to eliminate contaminating bacteria that were enriched in the dead P. micropora cell aggregates larger than 20 micrometer. After washing the cells with fresh culture medium, the RNAs were extracted using Trizol reagent (Life technologies). Equal amounts of the RNA samples acquired from each time-point's cells were mixed and the mixtures were used in RNA-seq, Iso-Seq and TSS-seq analysis.
description
Japan:Ibaraki, Tsukuba
geo loc name
pond
isolation source
Paulinella micropora
http://purl.obolibrary.org/obo/NCBITaxon_1928728
organism
P. micropora RNA
sample name
MYN1
strain
P. micropora RNA
title