characteristics.text: SAMN17190246
characteristics.characteristic: External Id
characteristics.text: Salmonella genetic resources interest group
characteristics.characteristic: INSDC center name
characteristics.text: 2022-01-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-27T04:22:50.809Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS22817854
characteristics.characteristic: SRA accession
characteristics.text: 2020
characteristics.characteristic: collection date
characteristics.text: RNA were extracted from liquid culture using a Qiagen kit, then treated with DNAse I (NEB) and purified with phenol-chlorophorm method. RNAseq libraries were prepared with the protocol for library preparation of Intact RNA using NEBNext rRNA depletion kit (Bacteria)(NEB#E7850, NEB#78860) and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760, NEB#E7765) from 250ng per sample. Library were barcoded with dual index from NEB Multiplex Oligos for Illumina (96 Unique Dual Index primers Pairs set 4, E6446S). The sequencing of the pooled libraries was performed on a Nextseq apparatus. All these steps were done in biological replicates, so total libraries in this data sample= 16.
characteristics.characteristic: description
characteristics.text: USA: Boston
characteristics.characteristic: geo loc name
characteristics.text: Culture of the strains
characteristics.characteristic: isolation source
characteristics.text: Salmonella enterica subsp. enterica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_59201
characteristics.characteristic: organism
characteristics.text: RNA
characteristics.characteristic: sample type
characteristics.text: descendants of STK005
characteristics.characteristic: strain
characteristics.text: enterica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_28901
characteristics.characteristic: sub species
characteristics.text: RNA of 8 engineered strains (ER3625, ER3722, ER3718, ER3728, ER3721, ER3734, ER3729, ER3716)
characteristics.characteristic: title