characteristics.text: SAMN19966011
characteristics.characteristic: External Id
characteristics.text: Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain
characteristics.characteristic: INSDC center name
characteristics.text: 2024-06-30T00:52:19.333Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-30T00:52:19.333Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS22820268
characteristics.characteristic: SRA accession
characteristics.text: expansion
characteristics.characteristic: dev_stage
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: pooled male and female
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Organoids from passage 2 (day 8) in expansion medium were collected and incubating with 0.125% trypsin for 25 min and dissociated into single cells by mechanical pipetting. Nuclei were stained with DAPI (Sigma) on ice for 10 minutes, then cells were sorted using a MoFlo SX FACS cytometer (Beckman Coulter). Sorted single, living cells were resuspended in 40 mL of fresh cell media.
characteristics.characteristic: stud_book_number
characteristics.text: cochlea
characteristics.characteristic: tissue
characteristics.text: Expansion
characteristics.characteristic: title