characteristics.text: SAMD00019994
characteristics.characteristic: External Id
characteristics.text: University of Tokyo Graduate School of Frontier Sciences Department of Medical Genome Sciences Laboratory of Social Genome Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2021-08-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T01:50:32.317Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS200394
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS200394
characteristics.characteristic: SRA accession
characteristics.text: The E. coli strain used was BHY173, a MG1655 derivative with mutations in restriction enzyme genes (genotype: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA). BHY187: BHY173(pACYC184deltatet). This strain was grown in Lennox LB up to mid-exponential phase (O.D.600 =0.5) in two different batches. Then cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit (Gram-negative bacteria)(Epicentre). cDNA library for mRNA-seq was constructed using TruSeq RNA Sample Prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq 2000 platform (Illumina).
characteristics.characteristic: description
characteristics.text: MG1655 delta(mrr-hsdRMS-mcrB) deltamcrA
characteristics.characteristic: genotype
characteristics.text: Escherichia coli str. K-12 substr. MG1655
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_511145
characteristics.characteristic: organism
characteristics.text: BHY187-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006539
characteristics.characteristic: sample name
characteristics.text: BHY173
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006539
characteristics.characteristic: strain
characteristics.text: MG1655
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0002234
characteristics.characteristic: substrain
characteristics.text: BHY187-1
characteristics.ontologyTerms: http://www.ebi.ac.uk/efo/EFO_0006539
characteristics.characteristic: title