characteristics.text: SAMN26497762
characteristics.characteristic: External Id
characteristics.text: Bar Ilan University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-05-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-05-09T08:39:02.234Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS13738925
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS13738925
characteristics.characteristic: SRA accession
characteristics.text: To purify poly (A) RNA, total RNA was incubated with Dynabeads® mRNA DIRECT™ (Invitrogen) as per manufacturer’s instruction and poly (A) selected RNA was subjected library preparation. Poly (A) RNA (~800 ng) was fragmented and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), cleaned by Agencourt RNA clean XP beads (Beckman Coulter) and ligated to a 3’ linker using high concentration T4 RNA Ligase 1 (NEB) in a buffer containing DMSO, ATP, PEG 8000 and RNase inhibitor (NEB). The ligated RNA was cleaned from excess linker using Dynabeads® MyOne™ SILANE beads (Thermo Scientific), and first strand cDNA was prepared using the AffinityScript Reverse Transcriptase enzyme (Agilent). The RNA was subsequently degraded using 2 l of 1 M NaOH and the cDNA was cleaned using Dynabeads® MyOne™ SILANE beads (Thermo Scientific). The cDNA was further ligated to a 3’ adapter using high concentration T4 RNA Ligase 1 (NEB) and cleaned of excess adapter by using Dynabeads® MyOne™ SILANE beads (Thermo Scientific). The adapter ligated cDNA was PCR enriched using NEBNext® High-Fidelity (NEB) polymerase (9 PCR cycles) and sequenced in a Nextseq system (Illumina) in paired end mode (40 million reads for each sample).
characteristics.characteristic: description
characteristics.text: Promastigote
characteristics.characteristic: dev_stage
characteristics.text: Leishmania major was maintained in M199 medium at 26°C.
characteristics.characteristic: growth_protocol
characteristics.text: NA
characteristics.characteristic: isolate
characteristics.text: Leishmania major
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5664
characteristics.characteristic: organism
characteristics.text: Polysomal poly (A) RNA
characteristics.characteristic: sample_type
characteristics.text: not applicable
characteristics.characteristic: sex
characteristics.text: Friedlin
characteristics.characteristic: strain
characteristics.text: not applicable
characteristics.characteristic: tissue
characteristics.text: Model organism or animal sample from Leishmania major
characteristics.characteristic: title
characteristics.text: LM32Cs3H1 sKO
characteristics.characteristic: treatment