characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA114720173
characteristics.characteristic: External Id
characteristics.text: Philipps University Marburg, Core Facility Bioinformatics
characteristics.characteristic: INSDC center name
characteristics.text: 2023-11-24T22:13:10Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17139032
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13570:BL360
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: KPC
characteristics.characteristic: cell_line
characteristics.text: organoid
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: (Mouse organoids from Bag6 WT, Bag6 KO and PBS control groups were collected in Eppendorf tubes (n=3 per each group). Organoids were dissociated in cold PBS to remove the Matrigel. Cells were washed twice with cold PBS and centrifuged at 300 xg for 5 minutes at 4°C. Pancreatic cancer organoids were derived from the KPC mouse model according to published procedures (1). The KPC organoids (5,000 cells in 20 µL matrigel per 96 well) were cultured in organoid growth medium (1) plus the secretome (ratio 1:1) derived from mouse mast cells pre-treated according to the following conditions: 1. PBS: Mast cells pre-treated with PBS (control). 2. KO-EVs: Mast cells pre-treated with Bag6 KO EVs. 3. WT-EVs: Mast cells pre-treated with Bag6 WT EVs. After 5 days, phase contrast images were taken by confocal microscope (Leica DMI3000 B) and cell percentage was assessed using the Cell Titer 3D GLO kit (Promega, Walldorf, Germany), control PBS was set to 100%. RNA was extracted at the same timepoint. References: (1). Boj SF, Hwang CI, Baker LA, et al. Organoid models of human and mouse ductal pancreatic cancer. Cell 2015;160:324-38. Subsequently, the cells were lysed, and total RNA was isolated using the RNeasy Plus kit (Qiagen, Hilden, Germany, [Cat. No. / ID: 74034]) according to the manufacturer's instructions). Lexogen QuantSeq according to Manufacture's Instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: pancreatic carcinoma
characteristics.characteristic: disease
characteristics.text: Kras+/LSL-G12D; Trp53+/LSL-R172H; Pdx1-Cre
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: conditioned media of WT Mast cells
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: pancreatic duct
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: BL360
characteristics.characteristic: title