characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115120775
characteristics.characteristic: External Id
characteristics.text: School of Health and Life Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2024-01-19T17:04:55Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17768758
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13719:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: African palm weevil
characteristics.characteristic: common name
characteristics.text: Protocols: African Palm weevil larvae were collected from Nigeria and stored in nucleic acid preservation (NAP) buffer. Larvae were dissected and guts were separated into foregut, midgut and hindgut samples. Bacterial DNA extraction was performed from each sample in duplicates using the QIAamp DNA microbiome kit Qiagen) according to the manufacturer’s protocol. DNA concentration and purity were assessed using a Nanodrop and stored at 40C until processing. The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified from bacterial DNA. Sequencing libraries were prepared using the Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina). The libraries were purified, diluted to 6nm and pooled.
characteristics.characteristic: description
characteristics.text: larval stage
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Y
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Rhynchophorus phoenicis
characteristics.characteristic: organism
characteristics.text: Rhynchophorus phoenicis
characteristics.characteristic: organism
characteristics.text: foregut
characteristics.characteristic: organism part
characteristics.text: Rhynchophorus phoenicis
characteristics.characteristic: scientific_name
characteristics.text: Sample 2
characteristics.characteristic: title