characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: KU Leuven
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18930317
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14008:EXT358
characteristics.characteristic: Submitter Id
characteristics.text: 24
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Pituitary stem cell-derived organoids
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Organoids were collected using ice-cold Advanced DMEM/F-12 and centrifuged for 5 minutes at 190 x g at 4°C. Organoid pellets were then dispersed into single cells using TrypLE Express (Thermo Fisher Scientific) and mechanical dissociation, and filtered through a 40 µm filter. The cell suspension was centrifuged again (10 minutes at 190 x g at 4°C) and resuspended in PBS with 0.04% BSA at a concentration of 1x10^6 cells/mL. The single-cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3’ V3.1 Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA), aiming for an estimated 8,000 cells per library. Single-cell libraries were prepared with the Chromium Single Cell 3’ V3.1 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit from 10X Genomics.
characteristics.characteristic: description
characteristics.text: mouse postnatal
characteristics.characteristic: developmental stage
characteristics.text: -/iDTR
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: EXT358
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Anterior pituitary
characteristics.characteristic: organism part
characteristics.text: 3
characteristics.characteristic: passage
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: EXT358
characteristics.characteristic: title