characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University of Edinburgh Centre for Discovery Brain Sciences
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18960740
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14015:wt214
characteristics.characteristic: Submitter Id
characteristics.text: 12.5
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: The dorsal telencephalon of E12.5 mouse embryos were collected in 1.5ml Eppendorff tubes and was shock frozen on dry ice and stored at -80C.  Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer’s instructions. RNA was eluated in 14 μl of RNAse free water.  Libraries were prepared from 1 μg of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490)
characteristics.characteristic: description
characteristics.text: E12.5
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: wt214
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: dorsal telencephalon
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: unknown
characteristics.characteristic: sex
characteristics.text: C57Bl6
characteristics.characteristic: strain
characteristics.text: wt214
characteristics.characteristic: title