characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: The University Of Liverpool
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21391370
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14577:Scramble Stim Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: AB1167
characteristics.characteristic: cell_line
characteristics.text: skeletal muscle myotube
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Harvesting for both rt-QPCR and RNA sequencing was undertaken as follows. DM media was removed, and myotubes washed twice with PBS. The cells were harvested via scraping into the DPBS and cells were transferred to an Eppendorf tube and centrifuged as above and supernatant removed. The pellet was then re-suspended in 350 μl of Qiagen RLT lysis buffer and disrupted by gentle pipetting. Three hundred and fifty μl of 70% ethanol was added and RNA was extracted using the Qiagen mini-prep RNA extraction kit following manufacturer’s instructions. Extracted RNA was stored in 30µl of RNA free purified water at -80°C until needed.  RNA was extracted using the Qiagen mini-prep RNA extraction kit RNA was depleted from samples using Ribo-ZeroTM rRNA removal kit, following the manufacturer’s instructions. DNA free RNA was used for polyadenylation using the NEB Next poly(A) mRNA magnetic isolation module (New England Biolabs, Ipswich, UK).
characteristics.characteristic: description
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: electrical 15 min
characteristics.characteristic: stimulus
characteristics.text: Scramble Stim Sample 5
characteristics.characteristic: title