characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000035
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Treatment involving an exposure to watering frequencies.
characteristics.characteristic: Experimental Factor description
characteristics.text: Watering regime
characteristics.characteristic: Experimental Factor type
characteristics.text: week 1: 1350 mL of water, weeks 2-3: 2100 mL of water
characteristics.characteristic: Experimental Factor values
characteristics.text: Centro de Investigacion y Tecnologia Agroalimentaria de Aragon
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: EO_0007383
characteristics.characteristic: Plant Environment Ontology
characteristics.text: ERS19034035
characteristics.characteristic: SRA accession
characteristics.text: L. homblei_2
characteristics.characteristic: Submitter Id
characteristics.text: 4 month
characteristics.characteristic: age
characteristics.text: 2021-05-04
characteristics.characteristic: collection date
characteristics.text: L. homblei
characteristics.characteristic: common name
characteristics.text: L. homblei
characteristics.characteristic: genotype
characteristics.text: Spain
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: single_plant
characteristics.characteristic: observation_unit_description
characteristics.text: Lactuca homblei
characteristics.characteristic: organism
characteristics.text: Leaf
characteristics.characteristic: plant_anatomical_entity_name
characteristics.text: Total RNA extraction from lyophilized samples coming from 12 samples (2 accessions x 2 irrigation regimes x 3 biological replicates) was performed using the NZY Total RNA Isolation kit (NZYtech Lda.-Genes and Enzymes, Lisbon, Portugal). RNA was treated with DNase using the TURBO DNA-freeTM kit (Invitrogen, Waltham, MA, USA), following the manufacturers’ instructions. RNA quantity and purity were assessed with the Eukaryotic Total RNA Nanobioanalyzer Assay in a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA)
characteristics.characteristic: protocol
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Lactuca homblei
characteristics.characteristic: scientific_name
characteristics.text: adiazb@cita-aragon.es
characteristics.characteristic: study_contact_email
characteristics.text: Aurora Diaz Bermudez
characteristics.characteristic: study_contact_name
characteristics.text: L. homblei sample 2, control condition, harvest 3 weeks after the start of the treatment
characteristics.characteristic: title
characteristics.text: Conventional green house CO_715:0000190
characteristics.characteristic: type_of_growth_facility