characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA113904801
characteristics.characteristic: External Id
characteristics.text: Biozentrum, University of Basel
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-21T11:19:07Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15898968
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13099:MCF7_SOX2_OE_Full_Rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MCF7
characteristics.characteristic: cell_line
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were propagated to 70-90% confluence in T175cm2 flasks, incubated with cycloheximide (100 µg/ml, C7698, Sigma) 30min prior to harvest (scraping), washed (2x) with cold PBS buffer and resuspended in 400µl of 1x lysis buffer (#9803, CST) supplemented with protease/phosphatase inhibitor cocktail (#78442, Thermo Fisher) and 5mM of MgCl2 (HN03.1, ROTH). Cells were mechanically disrupted by vortexing (3x 10sec at max. output, 10min on ice in between), cleared by centrifugation (10min, 21.000rcf, 4°C) Hot phenol extraction of RNA SMARTer smRNA-Seq Kit for Illumina, Takara Bio
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: breast carcinoma
characteristics.characteristic: disease
characteristics.text: sox2 full length overexpression
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MCF7_SOX2_OE_Full_Rep2
characteristics.characteristic: title