characteristics.text: SAMD00233501
characteristics.characteristic: External Id
characteristics.text: Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T04:44:52.840Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS244196
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS244196
characteristics.characteristic: SRA accession
characteristics.text: Synchronized L1 larvae of KH1999: yb1999 IV; ybIs1965 [myo-2::UNC-32E3-E5(deltaE4aE4b)-mRFP-B1B2 myo-2::UNC-32E3-E5(-1)(deltaE4aE4b)-EGFP-B1B2 lin-15 (+)] X were fed with an E. coli strain OP50 in S-complete medium for 3 hours at 20 degree C. The worms were harvested and washed with M9 and total RNAs were extracted from the whole bodies by using RNeasy Plus Mini (QIAGEN). Poly(A)+ RNAs were then purified with Magnosphere UltraPure mRNA Purification Kit (Takara) and subjected to RNA-seq library preparation with SMARTer Stranded RNA-Seq Kit (Clontech).
characteristics.characteristic: description
characteristics.text: L1 fed for 3 hours
characteristics.characteristic: dev stage
characteristics.text: yb1999 IV; ybIs1965 [myo-2::UNC-32E3-E5(deltaE4aE4b)-mRFP-B1B2 myo-2::UNC-32E3-E5(-1)(deltaE4aE4b)-EGFP-B1B2 lin-15 (+)] X
characteristics.characteristic: genotype
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Defective in unc-32 exon 4c selection in the pharynx (Orange)
characteristics.characteristic: phenotype
characteristics.text: w34018
characteristics.characteristic: sample name
characteristics.text: KH1999
characteristics.characteristic: strain
characteristics.text: whole
characteristics.characteristic: tissue
characteristics.text: w34018_KH1999_L1_PolyA+_Rep1_Index11
characteristics.characteristic: title