characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Philipps University Marburg
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS19358992
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14053:NE001
characteristics.characteristic: Submitter Id
characteristics.text: 60 to 65
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: natural killer cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: First, frozen patient peritoneal cells were thawed and counted.  Next, Natural Killer (NK) cells were purified using the negative selection kit from Miltenyii (130-092-657), according to the manufactures protocol. In order to increase the purity, NK cells underwent a second round of purification using the positive selection kit from Miltenyii (130-050-401), according to the manufactures protocol. The purity was determined via FACS and microscopy and the NK cells were then lysed in 1 ml Trizol. RNA was isolated using Trizol/TriFast (30-2010), according to the manufacturers' protocol.  DNA was removed using the DNA-free™ DNA Removal Kit (Ambion, AM1906), according to the manufacturers' protocol. Illumina Truseq mRNA stranded, according to manufacturers' instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: high grade ovarian serous adenocarcinoma
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: OVCA188
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: ascitic fluid
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: NE001
characteristics.characteristic: title