characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Guizhou medical university
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21379220
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14512:CD630-1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: The WT, ΔrpoN, and ::rpoN strains were inoculated into BHIS medium and cultured until the OD600 reached 0.6. The cultures were then centrifuged at at 13,500 × g for 10 minutes, the supernatant was removed, and the bacterial pellets of each strain were collected. The samples were transported with adequate dry ice in an foam box to Nanjing Personalomics Co., Ltd. for transcriptome sequencing. Total RNA was extracted from WT, ΔrpoN, and ::rpoN using a bacterial total RNA extraction kit (DP430, TIANGEN, Beijing). Once the samples were deemed satisfactory, the following steps were conducted for sequencing: (1) Removal of ribosomal RNA; (2) Enrichment and purification of mRNA; (3) Fragmentation of mRNA; (4) Construction of the sequencing library and quality assessment of the library.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Clostridioides difficile
characteristics.characteristic: organism
characteristics.text: Clostridioides difficile
characteristics.characteristic: organism
characteristics.text: Clostridioides difficile
characteristics.characteristic: scientific_name
characteristics.text: Clostridioides difficile 630
characteristics.characteristic: strain
characteristics.text: CD630-1
characteristics.characteristic: title