characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: St-Patrick Research Group in Basic Oncology, Cancer Division of the Quebec University Hospital Research Centre, Laval University, Quebec, Canada.  Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Laval University, Quebec, Canada. 3 Cancer Research Centre, Laval University, Quebec, Canada.
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18296961
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13820:RNAseq_C8dox100Re_D7_Rep3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Clone8
characteristics.characteristic: cell_line
characteristics.text: mouse embryonic fibroblast cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: TTF1 conditional Clone C8 was cultured for >7days with 100ng.ml-1 doxycycline (Dox100), cells were then either harvested or cultures continued for a further 3, 5 or 7 days without doxycycline (Dox0). Cultures were then either harvested or 100ng.ml-1 doxycycline readded and cultures maintained for a further 3, 5, and 7 days (Dox100-Readdition). Total RNA was extracted from these cultures and from parallel cultures of NIH3T3 using Trizol and GeneJet RNA micro kit (ThermoFisher Scientific). RNA sequencing libraries were created using the NEBNext mRNA stranded protocol (New England Biolabs), (McGill University and Genome Quebec Innovation Centre).
characteristics.characteristic: description
characteristics.text: embryonic stage 14
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: TTF1-/-//Neo+Tre3G-3FLAGmTTF1
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 7 days DMEM 10%FBS
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: embryonic structure
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: RNAseq_C8dox100Re_D7_Rep3
characteristics.characteristic: title