characteristics.text: 2
characteristics.characteristic: Block
characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000022
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115029594
characteristics.characteristic: External Id
characteristics.text: INRA-CMSE
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-14T10:47:30Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17476430
characteristics.characteristic: SRA accession
characteristics.text: EFELE_2017_03_PRO_203_18S
characteristics.characteristic: Submitter Id
characteristics.text: 0N
characteristics.characteristic: Treatment
characteristics.text: terrestrial environmental zone
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2017
characteristics.characteristic: collection date
characteristics.text: 0.2
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: The present work monitored, over a decade, the cumulative effect of repeated digestate application on soil microbial communities employing and in-situ approach, comparing the effect of the digested feedstock to the same undigested feedstock, and to others fertilization treatments. The study was carried out at the experimental field site EFELE, located in Rennes, France. The experiment was set up in 2012 and is based on a wheat-maize crop rotation. A mineral fertilizer (MIN), and three different OWP: cattle manure (CM), pig slurry (PS), and pig slurry anaerobic digestate (PS-DIG), were applied once every year since the beginning of the trial to the present. These treatments were compared to a control (0N) that had not received any organic or mineral N input. To monitor soil microbial community dynamics, from 2012 to 2022, soil samples were collected every year twelve months after the last fertilization treatment. For the molecular analyses performed in this study, were selected soil samples belonging to the beginning of the trial (2012), and wheat crop years (2013, 2015, 2017, 2019, and 2022). All the molecular analyses were conducted in 2022; we used high-throughput sequencing targeting 16S and 18S ribosomal RNA genes to describe the dynamics of microbial soil communities. Our results highlighted that over a decade of annual inputs of OWPs or mineral fertilizer, the soil chemical properties, the microbial biomass, and the composition and structure of soil microbial communities followed lasting modifications in all evaluated treatments. These changes were primarily explained by the time effect and, to a lesser extent, by the quality of the applied product. The temporal factor (year) exerted a strong influence on all measured parameters, potentially reflecting environmental, climatic, and seasonal effects that could mask the impact of digestate on soil microorganisms. Moreover, soil management practices appear to buffer the effects of different treatments, whether organic or mineral, on soil microbial quality indicators. The impact of digestate on soil microbial communities should be assessed considering associated agricultural practices.
characteristics.characteristic: description
characteristics.text: 34
characteristics.unit: m
characteristics.characteristic: elevation
characteristics.text: agricultural soil
characteristics.characteristic: environmental medium
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 48.102
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -1.7956
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: area of cropland
characteristics.characteristic: local environmental context
characteristics.text: F: 5'-TGTACTACTC-3'; R: 5'-AGACTCATGC-3'
characteristics.characteristic: multiplex identifiers
characteristics.text: soil metagenome
characteristics.characteristic: organism
characteristics.text: F: 5'-CGATAACGAACGAGACCT-3'; R: 5'-ANCCATTCAATCGGTANT-3'
characteristics.characteristic: pcr primers
characteristics.text: PRJEB71034
characteristics.characteristic: project name
characteristics.text: 1
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: soil metagenome
characteristics.characteristic: scientific_name
characteristics.text: 18S
characteristics.characteristic: target gene
characteristics.text: Dynamics of microbial soil communities over 10 years of repeated organic and inorganic fertilization
characteristics.characteristic: title