characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Brighton and Sussex Medical School
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS19358981
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14052:KG1a Detached 3 (K3D)
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: KG1a
characteristics.characteristic: cell_line
characteristics.text: acute myeloid leukaemia cell line
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: detached
characteristics.characteristic: culture condition
characteristics.text: Protocols: OCI-AML3 and KG1a cells were pre-treated with anti-CD44 for 1 hour and then added to the co-culture for 3 hours. Cells were also treated in monoculture. Following this, the EasySep™ human CD45 Positive Selection Kit and an EasySep™ magnet were used to isolate the AML cells as per the manufacturer’s instructions.  To ensure all AML cells were treated in an identical fashion, both attached, detached and AML cells in monoculture were selected. Following selection, the cells were immediately snap frozen and frozen at -80 degrees.  Total RNA was exctracted from attached (from co-culture), detached (from co-culture) and alone (from monoculture) OCI-AML3 and KG1a cells following anti-CD44 pre-treatment for 1 hour and co-culture for 3 hours. The RNA extraction was perfromed by Active Motif using the MagMAX™ mirVana™ Total RNA Isolation Kit according to the manufacturer's protocol. RNA quality was checked by RNA Screen tape and an RNA integrity number (RIN) score of >8.3 was used as a threshold. The samples were all treated with DNase. Illumina’s TruSeq Stranded mRNA Library kit (Cat# 20020594). Performed by Active Motif, according to the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: KG1a Detached 3 (K3D)
characteristics.characteristic: title