characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA112868505
characteristics.characteristic: External Id
characteristics.text: WASHINGTON UNIVERSITY
characteristics.characteristic: INSDC center name
characteristics.text: 2023-04-04T14:28:31Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14875160
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12826:Sample 15
characteristics.characteristic: Submitter Id
characteristics.text: 35
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Formalin fixed paraffin embedded (FFPE) tissue sections with minimal acute inflammation from the ileal margins were used for transcriptomic analyses. Total RNA was isolated from ileal margins with RNeasy FFPE Kit (QIAGEN) according to the manufacturer's protocol. Total RNA concentration and purity was obtained from an absorbance ratio at 260nm and 280nm. Total RNA quality was then determined by Agilent Tapestation according to manufacturer’s recommendations. Library preparation was performed by the Genome Technology Access Center (GTAC) at Washington University. RNA library was prepared according to library kit manufacturer’s protocol, indexed, and pooled.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: ulcerative colitis
characteristics.characteristic: disease
characteristics.text: Sample 15
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: ileum
characteristics.characteristic: organism part
characteristics.text: ileal resection margin
characteristics.characteristic: sampling site
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 15
characteristics.characteristic: title