characteristics.text: 2D
characteristics.characteristic: 2d or 3d
characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Computational Biology Program, Ontario Institute for Cancer Research, Toronto, ON, Canada Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22182074
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14611:Sample 16
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MCF 10A
characteristics.characteristic: cell_line
characteristics.text: epithelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: For 2D, 75,000 sgRNA-infected C3 MCF10A cells 818 92 were plated into four 12-well plates (Sarstedt 83.3921). The cells were grown for 24 hours and scraped into 100ul of RNA Lysis buffer (Zymo Research R1051), then stored in -80C prior to RNA extraction. For the 3D sphere formation assay in RMRP FMRE experiments, 15,000 MCF10A cells were plated on Matrigel-coated 24-well plates and grown in MCF10A Assay Media with regular media changes every other day for three weeks. After sphere formation, media was removed, cells were rinsed with cold PBS, spun down, and resuspended in Zymo Lysis buffer for RNA extraction. RNA samples were extracted from cells using the Zymo Quick-RNA Microprep Kit (Zymo Research R1051). After extraction, sample integrity was checked with RNA Qubit (Invitrogen Q32852) and the Bioanalyzer.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: non-malignant
characteristics.characteristic: disease
characteristics.text: N/A
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Scrambled
characteristics.characteristic: sgrna
characteristics.text: Sample 16
characteristics.characteristic: title