characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115057757
characteristics.characteristic: External Id
characteristics.text: Division of Infection and Immunity, University College London, London, UK
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-22T14:02:41Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17598253
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13672:477v1_S13
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Three groups of asymptomatic adults at risk of latent tuberculosis infection (household TB contacts, farm workers with occupational exposure to bovine TB and HIV-infected people attending an out-patient clinic) and living in or around Addis Ababa, Ethiopia, were invited to give a blood sample for processing. Principal exclusion criteria were age <18 years; symptoms of active TB; haemoglobin concentration <10 g/dL; and presence of any chest radiograph abnormality consistent with active TB. HIV-infected participants who did not have any contra-indication to IPT were offered a 6-month course of 300 mg isoniazid and pyridoxine 50 mg daily and invited to return to give a second blood sample for the same laboratory tests following treatment completion. 3 mL blood was collected into Tempus Blood RNA Tubes (Thermo Fisher Scientific) to stabilise total RNA for isolation and downstream gene expression analysis, and processed with Tempus Spin RNA Isolation Kits (Thermo Fisher Scientific) to obtain high-quality total RNA. TURBO DNA-free kits (Thermo Fisher Scientific) and GLOBINclear kits (Thermo Fisher Scientific) were then used to remove residual DNA and globin mRNAs, respectively. For genome-wide mRNA sequencing, complementary DNA libraries were generated using Kappa HyperPrep kits (Roche).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: HIVpos
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: AKS477
characteristics.characteristic: individual
characteristics.text: 0
characteristics.characteristic: is6110_cd34neg
characteristics.text: 15.256054
characteristics.characteristic: is6110_cd34pos
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: 0
characteristics.characteristic: rpob_cd34neg
characteristics.text: 4.575778
characteristics.characteristic: rpob_cd34pos
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 477v1_S13
characteristics.characteristic: title