characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA114268336
characteristics.characteristic: External Id
characteristics.text: Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-15T16:20:01Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS16257395
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13265:M25_D3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: YF-17D vaccinatio
characteristics.characteristic: clinical history
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Whole blood was collected from volunteers at -D3 (baseline), D0 (before vaccination), D1, D3, D7, and D10 post-vaccination and stored in Tempus blood RNA tubes (Thermofisher) at -80°C until use. Tempus blood RNA tubes were thawed at room temperature, and RNA was extracted using the Tempus spin RNA isolation kit (Thermofisher, catalog nunmber: 4380204), eluted in 90µL of RNase-free water according to the manufacturer's protocol. 1µg of total RNA was used for library preparation. poly(A) mRNA isolation was performed using Oligo(dT) beads. mRNA fragmentation was carried out using divalent cations and high temperature. Priming was performed using random primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was then performed using DNA clean beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: M25
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: M25_D3
characteristics.characteristic: title