characteristics.text: SAMD00233494
characteristics.characteristic: External Id
characteristics.text: Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-06-23T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T04:44:51.753Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS244189
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS244189
characteristics.characteristic: SRA accession
characteristics.text: Synchronized L1 larvae of pes-4 homozygotes from KH2504: pes-4 (yb2504)/+ III; ybIs2381 [myo-3::LEV-11E6E7aE8-GGS6-mCherry myo-3::LEV-11E6E7bE8-GGS6-EGFP pBSKII(-)] X with were picked up and washed with M9 and total RNAs were extracted from the whole bodies by using RNeasy Plus Mini (QIAGEN). Poly(A)+ RNAs were then purified with Magnosphere UltraPure mRNA Purification Kit (Takara) and subjected to RNA-seq library preparation with SMARTer Stranded RNA-Seq Kit (Clontech).
characteristics.characteristic: description
characteristics.text: L1 (unfed)
characteristics.characteristic: dev stage
characteristics.text: pes-4 (yb2504) III; ybIs2381 [myo-3::LEV-11E6E7aE8-GGS6-mCherry myo-3::LEV-11E6E7bE8-GGS6-EGFP pBSKII(-)] X
characteristics.characteristic: genotype
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Defective in lev-11 exon 7a selection in the head muscles
characteristics.characteristic: phenotype
characteristics.text: w34011
characteristics.characteristic: sample name
characteristics.text: KH2504
characteristics.characteristic: strain
characteristics.text: whole
characteristics.characteristic: tissue
characteristics.text: w34011_KH2504_L1_PolyA+_Rep2_Index4
characteristics.characteristic: title