characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: École Polytechnique Fédérale de Lausanne (EPFL)
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20302419
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14195:Kidney_HFD_NRH_000_M_rep_3
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: Kidney_H3
characteristics.characteristic: alternate name
characteristics.text: M14
characteristics.characteristic: barcode
characteristics.text: CTCCTGCGGTCCAA
characteristics.characteristic: barcode sequence
characteristics.text: 20240222
characteristics.characteristic: batch
characteristics.text: 384 V5A5 Odt
characteristics.characteristic: brbseq barcode kit
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Male and Female C57Bl/6NTac were purchased from Taconic. All mice were kept in a standard temperature- and humidity-controlled environment with a 12h:12h light-dark cycle. Mice had nesting material and ad libitum access to water and commercial diets. For the studies in Figures 1-3, mice were fed a regular mouse housing diet (Safe®150). For the high-fat diet study, we used D12492 from Research Diets, while using D12450J as a sucrose matched control. NRH was provided to mice in the drinking water, at a concentration of 0.1% of 0.4%, which approximately leads to a dose of 100 mg/kg*day or 400 mg/kg*day assuming mice drink around 3 ml per day and weight around 30 grams. Water bottles were renewed twice a week with a fresh solution. For the high-fat diet, the dose of NRH in the water bottles was adjusted weekly according to the average body weight of the cage. All animal experiments were carried out according to national Swiss and EU ethical guidelines and approved by the local animal experimentation committee under licenses VD2770.1, VD3647 and VD3841.  Animals were sacrificed after a 2 h fast, in order to stabilize systemic parameters and to allow the measurement of blood biochemistry in the fasting state. Tissues were collected upon sacrifice and flash-frozen in liquid nitrogen. Total mRNAs were extracted as described previously (Ratajczak, J. et al. Nat Commun, 2016) BRB-seq libraries were generated using a MERCURIUS BRB-seq kit (Alithea Genomics, #10813) according to the manufacturers’ instructions
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: HFD
characteristics.characteristic: diet
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: 20240222_BRBseq_Kidney_HFD
characteristics.characteristic: library
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Kidney
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57Bl/6NTac
characteristics.characteristic: strain
characteristics.text: Kidney
characteristics.characteristic: tissue
characteristics.text: Kidney_HFD_NRH_000_M_rep_3
characteristics.characteristic: title
characteristics.text: Control
characteristics.characteristic: treatment
characteristics.text: Demultiplexed sample
characteristics.characteristic: type