characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA114006718
characteristics.characteristic: External Id
characteristics.text: Center for Molecular Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2023-07-10T18:01:32Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS15990592
characteristics.characteristic: SRA accession
characteristics.text: Single cell_LFD AOM-DSS d81 (Epi_plus_DN)
characteristics.characteristic: Submitter Id
characteristics.text: 2021-05-13
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Wild type C57BL/6J male mice were fed with low fat diet diet throughout the experiment including 4 weeks before AOM injection. Mice were injected with 10mg/kg body weight azoxymethane (AOM). One week after AOM injection, mice were exposed to DSS in drinking water for 7 days followed by 7 days of recovery (DSS-cycle). This DSS cycle was repeated two more times for a total of 3 times with 7 days between each cycle. Following this, epithelial (EpCAM+CD45-, EPI), immune (EpCAM-CD45+, IMM) or stromal cells (EpCAM-CD45-, double negative, DN) cells were sorted from the colon and processed for single cell transcriptomics analysis using protocol from 10X genomics.
characteristics.characteristic: description
characteristics.text: Sweden
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Single cell_Low Fat Diet AOM-DSS d81 (Epi_plus_DN)
characteristics.characteristic: title