characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA13204332
characteristics.characteristic: External Id
characteristics.text: Garvan Institute of Medical Research and The Kinghorn Cancer Center, Darlinghurst, NSW, Australia
characteristics.characteristic: INSDC center name
characteristics.text: 2022-03-01T17:51:02Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS10807114
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-11487:SoftHydrogel_4T1_RNAseq_rep1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: 4T1
characteristics.characteristic: cell_line
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) and stiff (13.20±2.3 kPa) two-dimensional polyacrylamide hydrogels for 24hrs before being harvested for RNA extraction 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) two-dimensional polyacrylamide hydrogels for 24hrs RNA extraction was performed using the Qiagen RNAeasy extraction kit as per manufacturers recommendations The quality of the RNA was confirmed using TapeStation (Agilent), prior to library preparation using the KAPA mRNA HyperPrep Kit, as per the manufacturer protocol. Quality control was performed on the mRNA library using a LabChip GX prior to RNA sequencing.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: breast carcinoma
characteristics.characteristic: disease
characteristics.text: cultured on soft (1.24±1.3kPa) two-dimensional polyacrylamide hydrogels for 24 hours
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: mammary gland
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: BALB/c
characteristics.characteristic: strain
characteristics.text: SoftHydrogel_4T1_RNAseq_rep1
characteristics.characteristic: title