characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Instituto de Tecnologia Química e Biológica António Xavier - Universidade Nova de Lisboa (ITQB NOVA)
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22467214
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14647:R 12 hai rep1
characteristics.characteristic: Submitter Id
characteristics.text: 14
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: chickling vetch
characteristics.characteristic: common name
characteristics.text: PI268478
characteristics.characteristic: cultivar
characteristics.text: Protocols: The infected leaves were collected before inoculation and at 12, 48 and 72 hours after inoculation using liquid nitrogen. The samples were stored at -80 ºC until RNA extraction. Seedlings were grown in 0.5 L pots containing 250 cm3 of peat in a growth chamber at 20°C, with a 12-h light/12-h dark photoperiod until 14 days old. Fourteen-days old seedlings were inoculated with Erysiphe pisi isolate Ep-CO-01 using a settling tower to ensure uniform conidial deposition of 8 conidia/mm2. The samples 0 hai were not inoculated. Total RNA was isolated using the GeneJETTM Plant RNA Purification Mini Kit (ThermoScientificTM, Massachusetts, USA) according to the manufacturer’s instructions.  The RNA library construction was carried out using a Stranded mRNA Library Preparation Kit (Roche/KAPA mRNA HyperPrep kit).
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: Resistant
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Erysiphe pisi Ep-CO-01
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Lathyrus sativus
characteristics.characteristic: organism
characteristics.text: Lathyrus sativus
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Lathyrus sativus
characteristics.characteristic: scientific_name
characteristics.text: R 12 hai rep1
characteristics.characteristic: title