characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: UON
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20982048
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14402:WX126C
characteristics.characteristic: Submitter Id
characteristics.text: 57
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: 31.5
characteristics.characteristic: body mass index
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood with SepMate isolation tubes and Lymphoprep density gradient. PBMCs were then incubated with the following panel of antibodies; APC anti-human CD14 (Biolegend: 301807), Brilliant Violet 421 anti-human CD16 (Biolegend: 302037), PE anti-human CD66 (Biolegend: 342303), and PE/Cy7 anti-human HLA (Biolegend: 327017). Dead cells were excluded by staining with propidium iodide (Biolegend:421301). Flow cytometry and cell sorting was performed using a Beckman Coulter Astrios EQ cell sorter. Data were analysed with Kaluza Analysis software. Total RNA was extracted from cell suspensions of classical monocytes (CD14++/CD16-/CD66b-/HLA-DR+) and intermediate monocytes (CD14++/CD16+/CD66b-/HLA-DR+) using TRIzol reagent. Next generation RNA sequencing was performed by Genewiz. SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments. The final library was assessed with Agilent TapeStation.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: osteoarthritis, knee
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: WX126
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: 2
characteristics.characteristic: kellgren-lawrence radiographic knee oa score
characteristics.text: 7
characteristics.characteristic: numerical rating pain scale score
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Classical Monocyte
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Male
characteristics.characteristic: sex
characteristics.text: WX126C
characteristics.characteristic: title