characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Indian Institute of Technology - Hyderabad (IITH)
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21412232
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14591:HUVEC_HH313_SLC25A13kd
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HUVECs
characteristics.characteristic: cell_line
characteristics.text: human umbilical vein endothelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: We generated short hairpin RNA (shRNA) vectors against SLC25A13 and oligonucleotides were cloned into the pLKO-shRNA2 vector (PLKO). A nonsense scrambled shRNA sequence was used as a negative control. Lentiviral particles were produced in 293T cells. For lentiviral transduction of HUVECs, a multiplicity of infection (MOI) of 30 was used. Transductions were performed on day 0 and after 24 hours the cells were refed with fresh medium. RNA isolation experiments were performed from day 6 after transduction. Knockdown efficiency was monitored before RNA isolation.    RNA of scrambled control and SLC25A13KD ECs was extracted 6 days after seeding using the TRIzol kit (Life Technologies). Sequencing libraries were prepared with lllumina TruSeq Stranded mRNA Library prep kit according to the manufacturer's protocol. Samples were indexed to allow for multiplexing. Library quality and size range was assessed using a Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies) according to the manufacturer’s recommendations.
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: SLC25A13 knockdown
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HUVEC_HH313_SLC25A13kd
characteristics.characteristic: title