characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115165795
characteristics.characteristic: External Id
characteristics.text: Center of Clinical Research, Shanghai Children's Medical Center, Shanghai, 200127, PR China
characteristics.characteristic: INSDC center name
characteristics.text: 2024-01-30T22:15:00Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18008184
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13750:Normal 14
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Genomic DNA was extracted from the whole blood of children using a Blood DNA Extraction Kit (Tiangen Biotech, China), then treated with EpiTect Fast DNA Bisulfite Kit (QIAGEN, Germany) for bisulfite conversion. The converted DNA was PCR amplified with primer sequences designed to cover the CpGs in two promoter regions: each region included near 1,000 bp centering around the transcription start sites (TSSs) of SLC22A18. The PCR products were purified and used for DNA library preparation. According to the manufacturer's protocol, the library was prepared with the KAPA HTP Library Preparation Kit (KAPA Biosystems, USA).
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Normal 14
characteristics.characteristic: title