characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University College London Great Ormond Street Institute of Child Health
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22542184
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14604:Treatment 4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: human immortalised podocytes
characteristics.characteristic: cell_line
characteristics.text: podocyte
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cell lysates were collected by buffer RLT from Qiagen RNEasy kit after 24 hours of serum treatment Cells were treated with 10% serum from a patient with focal segmental glomerulosclerosis when they were presenting with FSGS (AP), on vincristine treatment (OT), or in remission (R) for 24 hours.  Qiagen RNEasy kit to extract RNA from cells. Cells are lysed, scraped off vessel, then exposed to spin columns and washes to extract RNA. A gDNAse step was also included.  KAPA mRNA HyperPrep kit to prepare the H. sapiens library for sequencing
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: focal segmental glomerulosclerosis with vincristine treatment
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: cortex of kidney
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Treatment 4
characteristics.characteristic: title