characteristics.text: 2024-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA112641202
characteristics.characteristic: External Id
characteristics.text: Department of Biological Sciences University of Bergen, Bergen, Norway
characteristics.characteristic: INSDC center name
characteristics.text: 2023-02-13T14:57:14Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14633177
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12606:I7-6
characteristics.characteristic: Submitter Id
characteristics.text: 1.5
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Atlantic cod
characteristics.characteristic: common name
characteristics.text: Protocols: The fish were sampled at 20 h or 48 h post injection. The fish were killed by a blow to the head. Liver samples were rapidly excised, snap frozen in liquid nitrogen and stored at -80°C for further processing. Liver total RNA extraction was performed from individual fish. A total of 32 individual liver RNA samples from groups of 20 h time point (Control, BPA (40 μmol/kg bw), MXC (40 μmol/kg bw) and EE2 (50 nmol/kg bw), n = 6 per group) and 48 h time point (Control, n = 3, EE2 = 5) were analyzed. RNA extraction was performed from each sample using TRI Reagent (Sigma) according to the manufacturer’s protocols. The concentration and quality of total RNA was assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA sequencing was performed at the Genomics Core Facility at the University of Bergen, as previously described (Yadetie et al., 2018), according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide on Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA).
characteristics.characteristic: description
characteristics.text: juvenile stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: Gadus morhua
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Gadus morhua
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: I7-6
characteristics.characteristic: title