SAMD00021018
External Id
Tokyo Medical and Dental University Medical Research Institute Gene Expression
INSDC center name
2022-09-01T00:00:00Z
INSDC first public
2024-06-02T01:50:43.693Z
INSDC last update
DRS254432
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS254432
SRA accession
The worm strain used was KH1775. This strain is mutant worm. The genotype is yb1688. Total RNAs were extracted from synchronized L1 worms by using RNeasy Midi kit (Qiagen). Poly (A)+ RNAs were repeatedly purified with FastTrack MAG (Invitrogen), fragmented in 1Â~ Fragmentation buffer (Ambion) and reverse transcribed using random hexamers and Primescript II (Takara). Second strand cDNAs were synthesized by using RNase H (Invitrogen) and DNA polymerase I (Invitrogen). Deep sequencing was performed by utilizing Genome Analyzer II (Illumina) following standard protocols.
description
L1
dev stage
yb1688
genotype
Caenorhabditis elegans
http://purl.obolibrary.org/obo/NCBITaxon_6239
organism
KH1775 L1
sample name
KH1775
strain
yb1688 L1 [RNAseq]
title