SAMN30916528
External Id
Coburg University of applied sciences and arts
INSDC center name
2022-09-23T00:00:00Z
INSDC first public
2024-02-08T09:00:56.848Z
INSDC last update
SRS15224828
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS15224828
SRA accession
replicate 5
biological replicate
2018-08
collection_date
A. m. mellifera were captured with tweezers and snap frozen directly in liquid nitrogen. The bees were stored at -80 degrees celsius until sample preparation.
description
Germany: University of Wuerzburg apiary
geo_loc_name
bees cuticular surface
isolation_source
49.77963710 N 9.97511451 E
lat_lon
insect metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1234904
organism
bees cuticular surface of A. m. mellifera5
ref_biomaterial
aerobe
rel_to_oxygen
Cuticle preparation occurred under frozen conditions. Antennae, legs, and wings were removed (but not discarded). Afterward, the inner organs (i.e., brain, muscles, gut, sting apparatus) were carefully removed from the head capsule, the thorax, and the abdomen. The prepared cuticles and outer body parts went into DNA isolation. Prepared cuticles from four bee individuals of each treatment were pooled for one sample. DNA extraction was performed using the Quick-DNA™ Fecal/Soil Microbe Microprep Kit according to the manufacturer's protocol.
samp_mat_process
Cuticule of 4 bees
samp_size
Cuticular metagenome A. m. mellifera5
title