SAMN31260619
External Id
Institut Curie
INSDC center name
2024-08-29T00:50:22.100Z
INSDC first public
2024-08-29T00:50:22.100Z
INSDC last update
SRS15387612
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS15387612
SRA accession
E4
age
Heard lab
biomaterial_provider
ESC_PGK
cell_line
embryonic stem cells
cell_type
Instittu Curie
collected_by
E4
dev_stage
Cells were maintained on gelatin-coated flasks in serum-containing ES cell medium (DMEM) supplemented with 15% ESC-grade foetal bovine serum, 1000U/ml leukemia inhibitory factor (LIF, Millipore) and 0.1mM B-mercaptoethanol in humidified atmosphere at 37C with 8% CO2.
growth_protocol
embryonic stem cells
isolate
Genomic DNA was extracted from cells using the DNeasy blood and tissue kit (Qiagen), following the manufacturers instructions. 1g of genomic DNA was used for preparation of the library.
isolation_source
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
cell culture
sample_type
female
sex
PGK
strain
derived from blastocyst
tissue
ESC_PGK12.1
title