SAMN30824257
External Id
Coburg University of applied sciences and arts
INSDC center name
2022-09-14T00:00:00Z
INSDC first public
2024-02-08T09:00:56.319Z
INSDC last update
SRS15123272
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS15123272
SRA accession
replicate 4
biological replicate
2021-03
collection_date
The honeybees were collected from a hive of the University of Wuerzburg. They were transferred into cages, which were maintained in an incubator (30°C, 50% humidity) for one week. The food was provided via Eppendorf cups that were prepared with little holes at the bottom. As a bee was expected to consume 60µl liquid per day, the amount of food per cage was adapted to the number of individuals, so that the bees could feed ad libitum. Each day, the Eppendorf cups were removed and replaced by new ones, so that a controlled and fresh food supply could be guaranteed. The feeding solutions were calculated on the basis of residue levels found in the field, which resulted in a concetentration of 100 µg*l-1 Steward® solved in 30% sugar water solution (based on sucrose).
description
Germany: University of Wuerzburg apiary
geo_loc_name
bees cuticular surface
isolation_source
49.77963710 N 9.97511451 E
lat_lon
insect metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1234904
organism
bees cuticular surface of Steward 4
ref_biomaterial
aerobe
rel_to_oxygen
Cuticle preparation occurred under frozen conditions. Antennae, legs, and wings were removed (but not discarded). Afterward, the inner organs (i.e., brain, muscles, gut, sting apparatus) were carefully removed from the head capsule, the thorax, and the abdomen. The prepared cuticles and outer body parts went into DNA isolation. Prepared cuticles from four bee individuals of each treatment were pooled for one sample. DNA extraction was performed using the Quick-DNA™ Fecal/Soil Microbe Microprep Kit according to the manufacturer's protocol.
samp_mat_process
Cuticule of 4 bees
samp_size
Bees cuticular metagenome Steward 4
title