SAMN46062329
External Id
NorthShore University HealthSystem
INSDC center name
2025-01-02T00:00:00Z
INSDC first public
2025-01-03T12:47:39.568Z
INSDC last update
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
not applicable
age
Duan lab, Room 241 Research Institute, 1001 University Place, IL, 60201, USA, NorthShore University HealthSystem
biomaterial_provider
CD0000003, CD0000007, CD0000010, CD0000053
cell_line
Neuron
cell_type
2022-04
collection_date
Library 53, 1 hr post stimulation, contains cell lines CD0000003, CD0000007, CD0000010, CD0000053
description
USA:Chicago
geo_loc_name
Cocultured neurons were briefly washed with 1x PBS and dissociated in Accutase at 37C for 40 min with gentle shaking After Accutase incubation we pipetted cells multiple times and filtered suspension twice with 40 um tip filter Sigma BAH13680004050EA to obtain single cells The washed cells were subjected to fresh nuclei isolation following 10x Genomics protocols CG000365 and CG000338 For each library around 15000 freshly isolated nuclei were loaded for GEM capture targeting 10000 nuclei recovery The preamplified DNAs for snATACseq and cDNAs for snRNAseq were shipped to the University of Minnesota Genomics Center UMGC for snmultiomics sequencing library construction following 10x Genomics standard protocol
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
10x Genomics single-cell multiomics (snRNA-seq + snATAC-seq) Library 53, 1 hr post stimulation, contains cell lines CD0000003, CD0000007, CD0000010, CD0000053
sample_type
not applicable
sex
cultured glutamatergic and GABAergic neurons derived from iPS cells
tissue
scARC library, group 53, 1hr
title