characteristics.text: SAMD00432597
characteristics.characteristic: External Id
characteristics.text: sekimoto lab, Faculty of Science, Japan Women's University
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-24T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T10:43:44.627Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS270374
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS270374
characteristics.characteristic: SRA accession
characteristics.text: National Institute for Environmental Studies
characteristics.characteristic: biomaterial provider
characteristics.text: Terunobu Ichimura
characteristics.characteristic: collected by
characteristics.text: NIES:67,68
characteristics.characteristic: culture collection
characteristics.text: For the vegetative cultures, mt+ (NIES-67) and mt- (NIES-68) cells were separately harvested at 6 (start time of light illumination is 0) and 18 h (IsoSeq 1). For the sexually-differentiated cells, vegetatively growing cells of the mt+ and mt- were separately collected, washed three times with MI medium, and incubated separately in MI medium (3.6E5 cells/ 72 mL in 300-mL Erlenmeyer flasks) under continuous light for 4 and 24 h (IsoSeq 2 and IsoSeq 3, respectively). For the mating-induced cells, cells of both mating types, which had been separately cultured in MI medium (at 3.6E5/ 72 mL in 300-mL Erlenmeyer flasks) for 24 h, were mixed and co-incubated (at 3.6E5 each/ 72 mL in 300-mL Erlenmeyer flasks) for 4 and 8 h (IsoSeq 4), 16 and 24 (IsoSeq 5), and 48 and 72 (IsoSeq 6).
characteristics.characteristic: description
characteristics.text: freshwater
characteristics.characteristic: env biome
characteristics.text: mating group I-E (NIES-67 and NIES-68)
characteristics.characteristic: note
characteristics.text: Closterium sp. mating group I-E
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_2796352
characteristics.characteristic: organism
characteristics.text: Iso-seq sequence of Closterium sp. mating group I-E
characteristics.characteristic: sample name
characteristics.text: Closterium sp. Mating group I-E from vegetative and mating cultures
characteristics.characteristic: title