characteristics.text: SAMN32083172
characteristics.characteristic: External Id
characteristics.text: University of Waterloo
characteristics.characteristic: INSDC center name
characteristics.text: 2022-12-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-09T13:32:51.466Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS18088576
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS/MIMS/MIMARKS.water
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.ba
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.ba.water.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS18088576
characteristics.characteristic: SRA accession
characteristics.text: 2017-09
characteristics.characteristic: collection_date
characteristics.text: 3.88 m
characteristics.characteristic: depth
characteristics.text: "Candidatus Chlorohelix allophototropha" is a mesophilic and phototrophic member of the Chloroflexota (formerly Chloroflexi) phylum cultivated from an iron-rich Boreal Shield lake (Canada). The species is currently (as of 2022) unique among the Chloroflexota phylum in its use of a Type I photosynthetic reaction center (RCI) to perform light energy conversion. Here, we picked a single, isolated colony of "Ca. Chx. allophototropha" from a non-axenic enrichment culture (subculture 19.9) and extracted its DNA. Based on 16S rRNA gene amplicon sequencing, only sequences of "Ca. Chx. allophototropha" were detectable in the sample (to a detection limit of ~0.03% relative abundance). Given that the sample was dominated by "Ca. Chx. allophototropha", we used the DNA from this sample to obtain the genome of "Ca. Chx. allophototropha".
characteristics.characteristic: description
characteristics.text: small freshwater lake biome [ENVO_00000892]
characteristics.characteristic: env_broad_scale
characteristics.text: freshwater environment [ENVO_01000306]
characteristics.characteristic: env_local_scale
characteristics.text: anoxic water [ENVO_01000173]|fresh water [ENVO_00002011]
characteristics.characteristic: env_medium
characteristics.text: Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area
characteristics.characteristic: geo_loc_name
characteristics.text: https://doi.org/10.1101/2020.07.07.190934
characteristics.characteristic: isol_growth_condt
characteristics.text: L227-S17
characteristics.characteristic: isolate
characteristics.text: Single colony of Candidatus Chlorohelix allophototropha from a non-axenic enrichment (subculture 19.9); enrichment culture was started from water from the upper anoxic zone (3.88 m depth) of an iron-rich Boreal Shield lake
characteristics.characteristic: isolation_source
characteristics.text: 49.69 N 93.69 W
characteristics.characteristic: lat_lon
characteristics.text: 5
characteristics.characteristic: num_replicons
characteristics.text: Candidatus Chlorohelix allophototropha
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3003348
characteristics.characteristic: organism
characteristics.text: https://doi.org/10.1101/2020.07.07.190934
characteristics.characteristic: ref_biomaterial
characteristics.text: Single colony of Candidatus Chlorohelix allophototropha from enrichment culture (subculture 19.9)
characteristics.characteristic: title
characteristics.text: reference material of Candidatus Chlorohelix allophototropha
characteristics.characteristic: type-material