characteristics.text: SAMN32912772
characteristics.characteristic: External Id
characteristics.text: University of Sao Paulo
characteristics.characteristic: INSDC center name
characteristics.text: 2024-08-23T00:50:19.557Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-23T00:50:19.557Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16583316
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS16583316
characteristics.characteristic: SRA accession
characteristics.text: Andre C. Lima
characteristics.characteristic: collected_by
characteristics.text: Self-supporting
characteristics.characteristic: dev_stage
characteristics.text: Brazil: Sao Paulo
characteristics.characteristic: geo_loc_name
characteristics.text: All analyses were performed with clones obtained from cuttings of a single plant of Bignonia magnifica cultivated at the gardens of the Biosciences Institute of the University of So Paulo, So Paulo (23.56 S, 46.73 W), Brazil, to avoid intraspecific variation. One-year-old, one-meter-tall plants were transplanted to 60 cm diameter by 50 cm high pots with soil rich in organic matter in April 2016. Plants were grown besides stainless-steel wire meshes (7x7 cm mesh, 2.1 mm diameter wire), that served as physical support for stem growth. Pots were set 1,5 m apart and watered to a maximum field capacity three times a week for the first year and weekly after that. Tissues were collected in April 2018 by peeling the bark and gently scraping the exposed xylem of 30 cm samples collected along the stems. Before tissue harvest, each stem segment had its base cut and stored in 50 % alcohol to determine the xylem phase, self-supporting or lianescent. Segments presenting dubious or transition anatomy, and the segment next to it, were discarded.
characteristics.characteristic: growth_protocol
characteristics.text: A single plant of Bignonia magnifica cultivated at the gardens of the Biosciences Institute of the University of So Paulo, So Paulo (23.56 S, 46.73 W) as described in the "growth protocol" field was used in the experiment.
characteristics.characteristic: isolate
characteristics.text: Sao Paulo city has a mean annual temperature of 21.5 o C and a mean total annual precipitation close to 1600 mm.
characteristics.characteristic: isolation_source
characteristics.text: 23.56 S 46.73 W
characteristics.characteristic: lat_lon
characteristics.text: Bignonia magnifica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_354051
characteristics.characteristic: organism
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Tissue sample
characteristics.characteristic: sample_type
characteristics.text: cambium and differentiating xylem
characteristics.characteristic: tissue
characteristics.text: Cambium and differentiating xylem, self-supporting, rep 2
characteristics.characteristic: title