characteristics.text: SAMN33196665
characteristics.characteristic: External Id
characteristics.text: Northeast Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-04-01T00:53:25.797Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-04-01T00:53:25.797Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS16708460
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS16708460
characteristics.characteristic: SRA accession
characteristics.text: 9 days of culture
characteristics.characteristic: age
characteristics.text: replicate = biological replicate 2
characteristics.characteristic: biological_replicate
characteristics.text: Northeast Agricultural University, Harbin, China
characteristics.characteristic: biomaterial_provider
characteristics.text: not collected
characteristics.characteristic: cell_line
characteristics.text: not collected
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collected_by
characteristics.text: not collected
characteristics.characteristic: collection_date
characteristics.text: P3
characteristics.characteristic: cultivar
characteristics.text: not collected
characteristics.characteristic: culture_collection
characteristics.text: Soybean overexpression of the aldehyde dehydrogenase AhALDH2B6 from Arachis hypogaea under 25 Celsius biological replicate 1
characteristics.characteristic: description
characteristics.text: germinating seed stage
characteristics.characteristic: dev_stage
characteristics.text: not collected
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: disease_stage
characteristics.text: not collected
characteristics.characteristic: ecotype
characteristics.text: not collected
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geo_loc_name
characteristics.text: Peanut, transgenic soybean, and WT seeds in the same growth state were selected. The seeds were disinfected with 70% (v/w) ethanol for 1 min and soaked in 5% (w/v) sodium hypochlorite solution for 15 min, after which the seeds were rinsed 5 to 6 times with sterile water for 5 to 6 times. Soak completely sterilized soybean seeds in 25 C sterile water in a dark growth chamber for 12 h, and peanut seeds need to be soaked for 24 h. For germination stage, The three transgenic strains and WT were seeded in vermiculite and then transferred into a chamber at lower temperature of 6 C, and, we performed RNA-seq analysis using line1-1 and WT, The radicles of transgenic and WT plants treated for 9 d in the cold chamber were used as samples for RNA-seq.
characteristics.characteristic: growth_protocol
characteristics.text: not collected
characteristics.characteristic: height_or_length
characteristics.text: Room temperature treatment of transgenic plants at 25 Celsius
characteristics.characteristic: isolate
characteristics.text: not collected
characteristics.characteristic: isolation_source
characteristics.text: 45.75 N 126 E
characteristics.characteristic: lat_lon
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: not collected
characteristics.characteristic: phenotype
characteristics.text: not collected
characteristics.characteristic: population
characteristics.text: not collected
characteristics.characteristic: sample_type
characteristics.text: not collected
characteristics.characteristic: sex
characteristics.text: not collected
characteristics.characteristic: specimen_voucher
characteristics.text: 25
characteristics.characteristic: temp
characteristics.text: radicles
characteristics.characteristic: tissue
characteristics.text: Soybean overexpression of the aldehyde dehydrogenase AhALDH2B6 from Arachis hypogaea under 25 Celsius biological replicate 1
characteristics.characteristic: title
characteristics.text: 25
characteristics.characteristic: treatment