SAMN34218595
External Id
St. Jude Children's Research Hospial
INSDC center name
2024-05-30T00:50:26.497Z
INSDC first public
2024-05-30T00:50:26.497Z
INSDC last update
SRS17332040
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS17332040
SRA accession
not applicable
age
not applicable
biomaterial_provider
Mouse Embryonic Fibroblasts (MEFs) expressing YFP-Parkin
cell_type
YFP-Parkin-MEFs were lentivirally transduced with the mouse CRISPR-Cas9 knockout (GeCKOv2) library at 0.3 MOI, treated or not with two concentrations of valinomycin (300 nM or 600 nM) for three rounds of treatment and enriched by clonogenic survival, for pools of cells expressing multiple gRNAs targeting the same gene. Genomic DNA was harvested and prepared for NGS
description
Embryonic
dev_stage
not applicable
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
Genomic DNA
sample_type
not applicable
sex
C57BL/6
strain
Embryo
tissue
Model organism or animal sample from Mus musculus
title
Experiment 2 Valinomycin 300 nM
treatment