SAMN34502976
External Id
Washington University School of Medicine
INSDC center name
2024-01-28T00:00:00Z
INSDC first public
2024-01-28T21:34:15.672Z
INSDC last update
SRS17619236
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS17619236
SRA accession
2019-03/2020-03
collection_date
Metagenomic DNA from fecal samples of ~20 cows were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into the pZE21 expression vector and transformed into E. coli. These E. coli transformant libraries were then screened against an antibiotic at a selective concentration. Resistant clones were collected from solid media; the plasmids were isolated and sequenced using Nextera protocol on Illumina NOVAseq 6000 platform.
description
P5
fmg_lib_id
USA: Marshfield, WI
geo_loc_name
Homo sapiens
host
stool
isolation_source
44.69 N 90.17 W
lat_lon
gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_749906
organism
tetracycline
selection_agent
Metagenome or environmental sample from gut metagenome
title