2025-01-10
ENA first public
ERC000011
ENA-CHECKLIST
VIB Center for Cancer Biology
INSDC center name
public
INSDC status
ERS21347597
SRA accession
E-MTAB-14554:Sample 4
Submitter Id
3
day
age
ArrayExpress
broker name
mixed
cell_type
not collected
collection date
house mouse
common name
Protocols: Growth plates were isolated from the distal femur and proximal tibia of 3-day-old mice (male wild-type: n=1; male G6PDH-null: n=1; female wild-type: n=1; female G6PDH-null: n=1) and the perichondrium was removed. To obtain a single cell suspension, the growth plates were digested with 5 mg/ml collagenase type II in minimum essential medium alpha GLUTAMAXTM supplemented with 100 units/ml penicillin, 50 µg/ml streptomycin and 10% FBS (all from Gibco, ThermoFisher Scientific) for 1.5 hours at 37°C. The cell suspension was passed through a 40 µm nylon mesh, centrifuged at 300g for 7 minutes at room temperature and resuspended in phosphate-buffered saline supplemented with bovine serum albumin.  Single cell suspensions were converted to barcoded scRNA-seq libraries using the Chromium Single Cell 3’ Reagent Kits v3 (10x Genomics), aiming for 5,000 cells per library.
description
postnatal day 3
developmental stage
G6pdh_KO
genotype
not collected
geographic location (country and/or sea)
4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
growth plates
organism part
distal femur and proximal tibia
sampling site
Mus musculus
scientific_name
male
sex
Sample 4
title