2025-01-10
ENA first public
ERC000011
ENA-CHECKLIST
UCL
INSDC center name
public
INSDC status
ERS21883845
SRA accession
E-MTAB-14599:THC006CD19
Submitter Id
19.41
year
age
ArrayExpress
broker name
B cell
cell_type
not collected
collection date
human
common name
Protocols: Peripheral blood was obtained by venipuncture, followed by PBMC isolation via Ficoll gradient. Cells were frozen in liquid nitrogen until use. Thawed PBMCs were sorted using FACS to select total CD19+ B cells PicoPure RNA extraction kit as per manufacturer's protocol Libraries were prepped with either the KAPA mRNA HyperPrep Kit or the TruSeq Stranded mRNA Library prep.
description
Post-pubertal
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Illumina TruSeq mRNA stranded v2
library construction kit
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
2
sequencing batch
male
sex
THC006CD19
title