2025-01-10
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Adolescent Rheumatology Versus Arthritis at UCL UCLH and GOSH, London, WC1E 6JF, UK Department of Ageing, Rheumatology & Regenerative Medicine, Division of Medicine, University College London, London, WC1E 6JF, UK
INSDC center name
public
INSDC status
ERS21154703
SRA accession
E-MTAB-14531:AHC053CD8(2)
Submitter Id
20.7
year
age
ArrayExpress
broker name
CD8+ T cells
cell_type
not collected
collection date
human
common name
Protocols: Peripheral blood was obtained by venipuncture, followed by PBMC isolation via Ficoll gradient. Cells were frozen in liquid nitrogen until use. Thawed PBMCs were sorted using FACS to select CD8+ T cells. PicoPure RNA extraction kit as per manufacturer's protocol TruSeq Stranded mRNA Library prep kit (Illumina cat. no. 20020595) OR KAPA mRNA HyperPrep Kit (Roche p/n KK8580), depending on sequencing batch
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Roche Kapa mRNA HyperPrep
library construction kit
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
4
sequencing batch
male
sex
AHC053CD8(2)
title