characteristics.text: SAMD00325921
characteristics.characteristic: External Id
characteristics.text: Department of Pharmacology School of Pharmaceutical Sciences, University of Shizuoka
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T07:57:08.723Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS309125
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS309125
characteristics.characteristic: SRA accession
characteristics.text: 13, 14, 15 weeks old
characteristics.characteristic: age
characteristics.text: SLC,Inc., Hamamatsu, Japan
characteristics.characteristic: biomaterial_provider
characteristics.text: primary mouse hepatic stellate cell
characteristics.characteristic: cell_line
characteristics.text: primary cell
characteristics.characteristic: cell_type
characteristics.text: 2020-07-22/2020-09-10
characteristics.characteristic: collection_date
characteristics.text: Primary hepatic stellate cells (HSCs) were isolated from 40 male ddY mice (Nihon SLC, 13?15 weeks old) by digestion with PRONASE (Merck Millipore) and collagenase (Yakult) and fractionated by 13% HistoDenz (Sigma) according to the method of our previous study (Yamaguchi M., Biol Pharm Bull., 2017). HSCs were identified by autofluorescence derived from vitamin A, which was excited at 340 nm and emitted at 550 nm. The purity of HSCs in each dish was confirmed to be greater than 90%. Isolated HSCs were seeded on culture dishes or multi-well plates in Dulbeccos modified Eagles medium low glucose (Sigma) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific) and Penicillin/Streptomycin (Meiji) and incubated for overnight at 37 ?C in a 95% air?5% CO2 atmosphere. On the 2nd day, prostaglandin E2 (PGE2) was added to the medium supplemented with 10% FBS. The medium with PGE2 was changed every 2 days thereafter. HSCs were cultured for 7 days.
characteristics.characteristic: description
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Day 7_PGE2
characteristics.characteristic: sample_name
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: liver
characteristics.characteristic: tissue
characteristics.text: Primary HSC_ Day 7_PGE2
characteristics.characteristic: title
characteristics.text: 1 ?M prostaglandin E2
characteristics.characteristic: treatment