characteristics.text: SAMD00327611
characteristics.characteristic: External Id
characteristics.text: Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T08:06:38.910Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS309118
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS309118
characteristics.characteristic: SRA accession
characteristics.text: 112 to 121 week
characteristics.characteristic: age
characteristics.text: CD150+CD48-Flt3-LSK cells cultured for 26 hours
characteristics.characteristic: cell_type
characteristics.text: 2018-11-14
characteristics.characteristic: collection_date
characteristics.text: CD150+CD48-Flt3- LSK cells from 112 to 121-week-old mice cultured with SCF 1ng/mL and TPO 100ng/mL in SF-O3+ 4%BSA medium for 26 hours. . A total of 4000 HSCs (for RNA-seq) or 36000 (for ATAC-seq) from collection of 3 C57BL/6J mice and a C57BL/6N mouse were sorted into 500uL of SF-O3+4%BSA and cultured. After culture, cells were subjected to RNA-seq or ATAC-seq. Biological replicate 1.
characteristics.characteristic: description
characteristics.text: wild type
characteristics.characteristic: genotype
characteristics.text: Healthy
characteristics.characteristic: health_state
characteristics.text: Bone marrow of bilateral femurs and tibiae
characteristics.characteristic: host_body_site
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Primary cell
characteristics.characteristic: passage_history
characteristics.text: Old_26h_1
characteristics.characteristic: sample_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL6/J and C57BL6/N
characteristics.characteristic: strain
characteristics.text: Bone marrow
characteristics.characteristic: tissue_type
characteristics.text: Old CD150+CD48-Flt3- LSK cells cultured for 26 hours. Biological replicate 1.
characteristics.characteristic: title