characteristics.text: SAMD00327608
characteristics.characteristic: External Id
characteristics.text: Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo
characteristics.characteristic: INSDC center name
characteristics.text: 2023-06-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T08:06:37.430Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS309115
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS309115
characteristics.characteristic: SRA accession
characteristics.text: 112 to 121 week
characteristics.characteristic: age
characteristics.text: CD150+CD48-Flt3-LSK cells
characteristics.characteristic: cell_type
characteristics.text: 2018-11-13
characteristics.characteristic: collection_date
characteristics.text: Freshly isolated CD150+CD48-Flt3- LSK cells from 112 to 121-week-old mice. A total of 3000 HSCs (for RNA-seq) or 30000 (for ATAC-seq) from collection of 3 C57BL/6J mice and a C57BL/6N mouse were sorted directly into 700uL RLT buffer + 3.5uL 2-ME (for RNA-seq) or 500uL SF-O3+4% BSA (for ATAC-seq). Biological replicate 1.Biological replicate 2.
characteristics.characteristic: description
characteristics.text: wild type
characteristics.characteristic: genotype
characteristics.text: Healthy
characteristics.characteristic: health_state
characteristics.text: Bone marrow of bilateral femurs and tibiae
characteristics.characteristic: host_body_site
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Primary cell
characteristics.characteristic: passage_history
characteristics.text: Old_Fresh_2
characteristics.characteristic: sample_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL6/J and C57BL6/N
characteristics.characteristic: strain
characteristics.text: Bone marrow
characteristics.characteristic: tissue_type
characteristics.text: Freshly isolated old CD150+CD48-Flt3- LSK cells. Biological replicate 2.
characteristics.characteristic: title