SAMN36864296
External Id
Institute of Genome Research
INSDC center name
2023-08-07T00:00:00Z
INSDC first public
2024-05-01T08:28:11.821Z
INSDC last update
SRS18544048
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.plant-associated
NCBI submission model
MIGS.eu.plant-associated.6.0
NCBI submission package
SRS18544048
SRA accession
2020
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
Plant
env_broad_scale
Catharanthus roseus
env_local_scale
Stem
env_medium
Viet Nam: Hanoi
geo_loc_name
Catharanthus roseus
host
Catharanthus roseus (L.) G. Don was obtained from herbal gardens in Hanoi, Vietnam. Plant materials from young, healthy, and pest-free plants were collected and stored in polyethylene bags at 4C. Surface sterilization of plant material and isolation of endophytic fungi were performed as described by Tan and colleagues. Stem, leaf, and root samples were washed with tap water to remove dust prior to surface disinfection with 70% (v/v) ethanol for 30 seconds and 2.5% (v/v) commercial bleach solution (Javel, Vietnam) for 20 minutes on a rotary shaker at 200 round per minute (rpm). All samples were then rinsed five times with sterilized distilled water and air-dried with sterile filter paper. Plant material was cut into small segments of approximately 2 cm in length using a sterile surgical blade and placed on potato dextrose agar (PDA; BD Difco, USA) medium containing 50 g/mL cefotaxime (Merck, USA). Tissues samples were kept at 25C in the dark for 5 days with daily checked for contamination. This step was conducted to eliminate bacteria and fungi on the surface of plant samples. The non-contaminated samples were crushed in a mortar and pestle before being placed on fresh PDA medium containing 50 g/mL cefotaxime and incubated at 25C in the dark until the endophytic fungal colonies started to grow on the media plate. Each isolated endophytic fungus was either cultured in PDA liquid medium for subsequent experiments or stored on a PDA slant.
isol_growth_condt
21.119914 N 105.834662 E
lat_lon
Talaromyces sp. DC2
http://purl.obolibrary.org/obo/NCBITaxon_3142527
organism
DC2
strain
MIGS Eukaryotic sample from Talaromyces sp. DC2
title