SAMN46178814
External Id
Southern medical university
INSDC center name
2025-01-09T00:00:00Z
INSDC first public
2025-01-12T00:15:20.257Z
INSDC last update
SRS23762578
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS23762578
SRA accession
18
age
14th Floor, New Laboratory Building, Southern Medical University, 1838 Guangzhou Avenue North, Baiyun District, Guangzhou City, Guangdong Province
biomaterial_provider
Dental pulp stem cell
cell_line
2022-01-20
collection_date
The overexpression plasmids of Polypyrimidine Tract Binding Protein 1 (PTBP1 or hnRNPI) was designed and constructed by OBiO Technology (Shanghai, China). The vector components used for overexpressing PTBP1 included pSLenti-SFH-EGFP-P2A-Puro-CMV-3xFLAG-WPRE.
description
China: Guangdong
geo_loc_name
cell type: Dental pulp stem cell-LV-PTBP1.The vector components used for overexpressing PTBP1 included pSLenti-SFH-EGFP-P2A-Puro-CMV-3xFLAG-WPRE. According to the manufacturers instructions, hDPSCs at passages 3-5 were seeded in cell culture flasks and transfected with the corresponding lentiviral vectors to generate stable cell lines for subsequent experiments. The transfection efficiency was validated by quantitative real-time quantitative PCR (qRT-PCR) and western blotting.
isolate
2
number
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
male
sex
dental pulp
tissue
Human sample from Homo sapiens
title