SAMN36746241
External Id
MicroGEM PrepGEM Bacteria Kit
Extraction_Method
PBCR2_S55_L001_R2_001.fastq.gz
Filename
PBCR2_S55_L001_R1_001.fastq.gz
Filename
fastq
Filetype
University of Auckland
INSDC center name
2024-08-01T00:53:53.870Z
INSDC first public
2024-08-01T00:53:53.870Z
INSDC last update
SRS18442686
INSDC secondary accession
live
INSDC status
Illumina MiSeq
Instrument model
paired
Library layout
PCR
Library selection
GENOMIC
Library source
AMPLICON
Library strategy
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
ILLUMINA
Platform
RNAlater
Preservation_ method
SRS18442686
SRA accession
feces
Source
2022-04-08
collection_date
Samples collected and pooled in polypropylene tubes and placed in ice, after which the samples were transported to the lab, pooled further and subsampled into smaller polypropylene tubes and preserved with either 95% ethanol and left at room temperature, or with RNAlater, stored overnight in 4° celsius and then placed in the freezer at -20° celcius. Samples were then processed through each of the five extraction approaches and the raw DNA extracts were evaluated for DNA Yield, Purity and Integrity. Subsequently all raw extracts were subjected through 16S rRNA gene amplification with PCR and sequenced by Auckland Genomics Ltd.
description
New Zealand
geo_loc_name
Gallus gallus domesticus
host
Home chicken coop
isolation_source
Not Collected
lat_lon
gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_749906
organism
PBCR2_S55
source_material_id
fecal 16S rRNA gene community
title