characteristics.text: SAMD00399155
characteristics.characteristic: External Id
characteristics.text: Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus
characteristics.characteristic: INSDC center name
characteristics.text: 2023-08-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-02T09:00:29.720Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS319934
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS319934
characteristics.characteristic: SRA accession
characteristics.text: Total RNAs were purified from hearts of an 18-week-old female Rbm20(S637A/S637A); Trp53 (KO/+) mouse and a 5-week-old female Rbm20(delta 26-nt/+) mouse with RNeasy Fibrous Tissue Mini Kit (QIAGEN) and reverse transcribed with oligo (dT) and PrimeScript II Reverse Transcriptase (TaKaRa). Ttn cDNA fragments were amplified with KOD One PCR Master Mix (TOYOBO) and following primer sets: 1) NanoporeMmTtn#29 5'-TTTCTGTTGGTGCTGATATTGCTGGGCACTCCTGAGAAATCAGAGTG-3' and NanoporeMmTtn#40 5'-ACTTGCCTGTCGCTCTATCTTCCCAGGTTGTAATGGCAGTGGATGGGGAGAC-3', 2) NanoporeMmTtn#29 and NanoporeMmTtn#46 5'-ACTTGCCTGTCGCTCTATCTTCATCTGGGGTCACATTGTGGATTGTC-3', 3) NanoporeMmTtn#59 5'-TTTCTGTTGGTGCTGATATTGCCGCATCCGGAGCATTAAGAAGGAGGTCCAG-3' and NanoporeMmTtn#46, 4) NanoporeMmTtn#59 and NanoporeMmTtn#40 and 5) NanoporeMmTtn#61 5'-TTTCTGTTGGTGCTGATATTGCTCTGCTACTTTCGAGTGCGAAGTGTC-3' and NanoporeMmTtn#38 5'-ACTTGCCTGTCGCTCTATCTTCCCTTTCAGCTTCTATTGGTGAAGGAG-3'. Each of the PCR products was further amplified with BP01 and BP02 Barcode primer provided in the PCR Barcoding Kit (Nanopore SQK-PBK004) for Rbm20(S637A/S637A); Trp53 (KO/+) and Rbm20(delta 26-nt/+), respectively, and pooled.
characteristics.characteristic: description
characteristics.text: Mixture of Rbm20(S637A/S637A); Trp53(KO/+) (Rbm20em1Hide/em1Hide; Trp53KO/+) and Rbm20(delta 26-nt/+) (Rbm20em3Hide/+)
characteristics.characteristic: genotype
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: yr0105
characteristics.characteristic: sample_name
characteristics.text: Heart
characteristics.characteristic: tissue
characteristics.text: Four-primer PCR sequencing of Ttn mRNAs from Rbm20 mutant mice heart.
characteristics.characteristic: title