characteristics.text: SAMN37298833
characteristics.characteristic: External Id
characteristics.text: National Institute of Allergy and Infectious Diseases
characteristics.characteristic: INSDC center name
characteristics.text: 2024-02-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-02-02T10:10:04.317Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS18814155
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Rm24h_R2
characteristics.characteristic: Library_ID
characteristics.text: Invertebrate
characteristics.characteristic: NCBI submission model
characteristics.text: Invertebrate.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS18814155
characteristics.characteristic: SRA accession
characteristics.text: 2021
characteristics.characteristic: collection_date
characteristics.text: Intact midguts (MG) from fully fed Rhipicephalus microplus adult females feeding on cattle were collected at 24h post-detachment and stored in RNAlater solution. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications.
characteristics.characteristic: description
characteristics.text: Brazil: RS, Porto Alegre
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: Rhipicephalus microplus ticks (Porto Alegre strain) were obtained from cattle maintained in insulated boxes at UFRGS (Brazil, RS). Calves were infested with larvae and after 21 days, partially engorged and fully engorged adult female ticks were collected.
characteristics.characteristic: isolate
characteristics.text: laboratory colony
characteristics.characteristic: isolation_source
characteristics.text: Rhipicephalus microplus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6941
characteristics.characteristic: organism
characteristics.text: Rhipicephalus microplus adult female midguts group 24h post-detachment replicate 2
characteristics.characteristic: replicate
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: midguts
characteristics.characteristic: tissue
characteristics.text: Rhipicephalus microplus adult female midguts group 24h post-detachment replicate 2
characteristics.characteristic: title