characteristics.text: 2025-01-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: UOM-BCF
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22535219
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14659:Sample 6
characteristics.characteristic: Submitter Id
characteristics.text: 8-10
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Animal maintenance and experiments were conducted in accordance with the United Kingdom Animals (Scientific Procedures) Act and approved by the University of Manchester Ethics Committee. Pontin gene-targeted mice were obtained from the European Conditional Mouse Mutagenesis Programme (EUCOMM). The targeted allele was generated using a “knock-out first” strategy (PMID 21677750). The exon 3 of mouse Ruvbl1 gene (Pontin) was targeted using a construct carrying FRT and loxP sites. Heterozygous mice carrying the conditional targeted allele were crossed with CAG-Flpo transgenic mice to remove the lacZ and neo markers resulting in clean Pontin floxed (Pon flox) mice. To generate a cardiomyocyte specific inducible KO we crossed homozygous Pon flox mice with αMHC-MerCreMer transgenic mice (αMCM) generating αMCM- Pon flox mice. Pontin inducible cardiomyocyte knockout (Pontin icKO) were obtained by injecting 8-10 weeks old male αMCM- Pon flox mice with 40 mg/kg BW of tamoxifen. Heart tissues from PontinicKO mice (αMCM-Pon flox + TAM) and control (αMCM + TAM) were collected at 1 week and 3 week following tamoxifen injection.  RNA was extracted using Trizol reagent. Sequencing library for RNA sequencing was prepared with TruSeq RNA Library Prep Kit v2 (Illumina), according to the manufacturer's protocols.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Control
characteristics.characteristic: genetic modification
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 6
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: Sample 6
characteristics.characteristic: title