characteristics.text: SAMN37229627
characteristics.characteristic: External Id
characteristics.text: U.S. Environmental Protection Agency
characteristics.characteristic: INSDC center name
characteristics.text: 2024-08-28T00:50:28.197Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-28T00:50:28.197Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS18767820
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS18767820
characteristics.characteristic: SRA accession
characteristics.text: 6-24hpf
characteristics.characteristic: age
characteristics.text: US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 56522
characteristics.characteristic: biomaterial_provider
characteristics.text: Adult zebrafish (AB wild type strain) were housed in an onsite culture facility at the US Environmental Protection Agency (USEPA) laboratory in Duluth, MN. The fish were raised in a flow-through system with control UV-treated, filtered Lake Superior water (LSW) at a flow rate of 250 mL/minute. Fish were held at 26 ± 1°C under a 16/8-hour light/dark photoperiod cycle. Fish were housed in 5.5-gallon tanks with <100 zebrafish per tank. Adult fish were fed live brine shrimp (Artemia) nauplii twice daily. The night prior, breeding zebrafish in groups of four males x six females were separated into breeding tanks containing a plastic screened spawning basket. The next morning, approximately two hours after the lights turned on, embryos were collected and maintained in filtered LSW in an incubator set at 28.5°C. At approximately 4 hours post fertilization (hpf), zebrafish embryos were screened under a stereo microscope, and only similarly staged embryos were sorted out for chemical exposure. All dead, malformed, and unfertilized eggs were discarded.
characteristics.characteristic: breeding_method
characteristics.text: Great Lakes Toxicology & Ecology Division
characteristics.characteristic: collected_by
characteristics.text: 2022
characteristics.characteristic: collection_date
characteristics.text: embryo
characteristics.characteristic: dev_stage
characteristics.text: 506.1838787429168
characteristics.characteristic: dose
characteristics.text: ug/L
characteristics.characteristic: dose_units
characteristics.text: USA: Minnesota, Duluth
characteristics.characteristic: geo_loc_name
characteristics.text: Danio rerio
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_7955
characteristics.characteristic: organism
characteristics.text: No
characteristics.characteristic: repeat_sample
characteristics.text: rep1
characteristics.characteristic: replicate_ID
characteristics.text: Total RNA
characteristics.characteristic: sample_type
characteristics.text: not applicable
characteristics.characteristic: sex
characteristics.text: AB wild type
characteristics.characteristic: strain
characteristics.text: 1
characteristics.characteristic: time_code
characteristics.text: whole body zebrafish embryo
characteristics.characteristic: tissue
characteristics.text: Model organism or animal sample from Danio rerio
characteristics.characteristic: title
characteristics.text: PFOS (CAS 1763-21-1)
characteristics.characteristic: treatment