SAMN38015666
External Id
IGMM- CNRS
INSDC center name
2024-12-02T00:50:25.030Z
INSDC first public
2024-12-02T00:50:25.030Z
INSDC last update
SRS19299485
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.6.0
NCBI submission package
SRS19299485
SRA accession
not applicable
collection_date
not applicable
cultivar
In vivo footprinting from a saturated culture using Sir3DamK9A, NLSDamK9A, Sir2EcoG2 or Sir4 EcoG2 and direct detection of GATC (Dam) or Adenine (EcoG2) methylation by nanopore sequencing (Oxford Nanopore). Fast5 files basecalled with guppy basecaller and dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg. Multiplexed library mix with the Rapid Barcoding Kit (RBK404: Sir3DamK9A;RBK405: NLSDamK9A;RBK410: Sir2EcoG2;RBK411: Sir2EcoG2).
description
not applicable
ecotype
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
not applicable
isol_growth_condt
saturated culture
isolate
not applicable
lat_lon
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Sir3DamK9A(MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR3:: Myc9-DAMK9A (methylase from E. coli) Phleomycin; NLSDamK9A(MATa ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1:: promoterSIR3-Myc9-NLSDAM K9A ::ADE2); Sir2EcoG2 (MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR2:: Myc9-EcoGII (methylase from E. coli) Phleomycin); Sir4EcoG2 (MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR4:: Myc9-EcoGII (methylase from E.coli) Phleomycin)
strain
Sir3damK9A_NLSDamK9A_Sir2EcoG2_Sir4EcoG2
title