SAMN38025741
External Id
Shengli Clinical Medical College of Fujian Medical University
INSDC center name
2024-12-12T00:50:25.437Z
INSDC first public
2024-12-12T00:50:25.437Z
INSDC last update
SRS19345557
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19345557
SRA accession
4 weeks
age
Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou, China
biomaterial_provider
Sprague-Dawley rat
breed
Bone marrow-derived mesenchymal stem cells
cell_type
Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou, China
collected_by
2022-07-15
collection_date
Not applicable
culture_collection
N-BMSCs are cultured under regular conditions (sample 1).
description
Juvenile rats
dev_stage
China: Fuzhou City
geo_loc_name
The femur and tibia of both legs were carefully extracted, and any surrounding soft tissue was meticulously removed. BMSCs were isolated from the bone marrow cavity by flushing with Dulbecco’s modified Eagle’s medium-F12 (DMEM-F12, Gibco, USA). The cell suspension was collected and centrifuged at 1500 rpm for 5 min, and resuspended in DMEM-F12 with 10% fetal bovine serum (FBS, Clark, USA). Cells were seeded with 4 mL of a cell suspension per flask and incubated at 37°C with 5% CO2. The culture medium was refreshed every 48 hours.Then, the culture medium was changed every 48 hours. When cells reached 90% confluence, they were subcultured using 0.25% trypsin-EDTA (Gibco, USA) and passaged in a 1:2 ratio.
growth_protocol
health
health_state
Rattus norvegicus
http://purl.obolibrary.org/obo/NCBITaxon_10116
organism
male
sex
Bone marrow
tissue
NBMSC1
title
Normal culturing
treatment