SAMN38015667
External Id
IGMM- CNRS
INSDC center name
2024-12-02T00:50:25.043Z
INSDC first public
2024-12-02T00:50:25.043Z
INSDC last update
SRS19299486
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.6.0
NCBI submission package
SRS19299486
SRA accession
not applicable
collection_date
not applicable
cultivar
In vivo footprinting from a saturated culture using Sir3EcoG2 with a sir4 or sir2 deletion, or NLSEcoG2 with a sir2, sir3 or sir4 deletion and direct detection of Adenine methylation by nanopore sequencing (Oxford Nanopore). Fast5 files basecalled with guppy basecaller and res_dna_r941_min_modbases-all-context_v001.cfg . Multiplexed library mix with the Rapid Barcoding Kit (RBK404: Sir3EcoG2sir2D;RBK405: Sir3EcoG2sir4D;RBK410: NLSEcoG2sir2D;RBK411: NLSEcoG2sir3D;RBK4112 NLSEcoG2sir4D).
description
not applicable
ecotype
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
not applicable
isol_growth_condt
saturated culture
isolate
not applicable
lat_lon
Saccharomyces cerevisiae
http://purl.obolibrary.org/obo/NCBITaxon_4932
organism
Sir3EcoG2_sir2D ((MATalpha ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR3:: Myc9-EcoGII (methylase from E.coli) Phleomycin sir2D::hygR); Sir3EcoG2_sir4D ((MATalpha ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 SIR3:: Myc9-EcoGII (methylase from E.coli) Phleomycin sir4D::HYGR); NLSEcoG2sir2D(MATa ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 MATa ade2-1::promotorSIR3-NLS-EcoGII::ADE2 sir2D::G418 ); NLSEcoG2sir3D(MATa ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 MATa ade2-1::promotorSIR3-NLS-EcoGII::ADE2 sir3D::G418 ); NLSEcoG2sir4D(MATa ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 MATa ade2-1::promotorSIR3-NLS-EcoGII::ADE2 sir4D::G418 )
strain
Sir3EcoG2sir2D_Sir3EcoG2sir4D_NLSEcoG2sir2D_NLSEcoG2sir3D_NLSEcoG2sir4D
title