SAMN38025742
External Id
Shengli Clinical Medical College of Fujian Medical University
INSDC center name
2024-12-12T00:50:25.463Z
INSDC first public
2024-12-12T00:50:25.463Z
INSDC last update
SRS19345556
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19345556
SRA accession
4 weeks
age
Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou, China
biomaterial_provider
Sprague-Dawley rat
breed
Bone marrow-derived mesenchymal stem cells
cell_type
Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou, China
collected_by
2022-07-15
collection_date
Not applicable
culture_collection
N-BMSCs are cultured under regular conditions (sample 2).
description
Juvenile rats
dev_stage
China: Fuzhou City
geo_loc_name
The femur and tibia from both legs were carefully extracted and stripped of surrounding soft tissue. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated by flushing with DMEM-F12 medium supplemented with 10% FBS. Cells were seeded in flasks and incubated at 37°C with 5% CO2, with the medium refreshed every 48 hours. Upon reaching 90% confluence, cells were subcultured in a 1:2 ratio using trypsin-EDTA.
growth_protocol
health
health_state
Rattus norvegicus
http://purl.obolibrary.org/obo/NCBITaxon_10116
organism
male
sex
Bone marrow
tissue
NBMSC2
title
Normal culturing
treatment