SAMN38044176
External Id
Tsinghua University
INSDC center name
2024-03-01T00:54:57.253Z
INSDC first public
2024-03-01T00:54:57.253Z
INSDC last update
SRS19716896
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19716896
SRA accession
2 months
age
China
birth_location
2022-07-11
collection_date
To achieve a deep quantitative proteomic profiling of whole tissue with the spatial localization preserved, we designed an untargeted spatial proteomics workflow with a sparse sampling strategy. We combined a computational approach and MS-based proteomics analysis to localize high-throughput proteome mapping within a tissue by microdissecting the slices into a series of parallel strips with different angles, that allow subsequent image reconstruction. We successfully reconstructed a whole-tissue mapping in a mouse brain, consisting of 8,645 protein distributions. Meanwhile, the spatial transcriptome data was also generated.
description
China
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
tissue sample
sample_type
female
sex
frozen
store_cond
C57BL6
strain
brain
tissue
Model organism or animal sample from Mus musculus
title