2025-01-16
ENA first public
ERC000023
ENA-CHECKLIST
SAMEA112329381
External Id
NETHERLANDS INSTITUTE OF ECOLOGY
INSDC center name
2023-01-16T13:45:18Z
INSDC last update
public
INSDC status
ERS14440291
SRA accession
SBR1-AGS-day53-18S
Submitter Id
0.015
L
amount or size of sample collected
Sequencing batch reactor
broad-scale environmental context
2021-01-15
collection date
Aerobic granular sludge
description
Synthetic wastewater
environmental medium
Netherlands
geographic location (country and/or sea)
51.98699968
DD
geographic location (latitude)
5.67116169
DD
geographic location (longitude)
Gelderland
geographic location (region and locality)
Granulated biomass, aerobic granular sludge
local environmental context
PCR
nucleic acid amplification
Qiagen DNAeasy Power Soil Pro
nucleic acid extraction
3
number of replicons
activated sludge metagenome
organism
aerobic
oxygenation status of sample
7.25
pH
616*F-1132R
pcr primers
Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org
project name
Sequencing batch reactor
reactor type
15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
sample material processing
0.2
g
sample volume or weight for DNA extraction
activated sludge metagenome
scientific_name
Illumnia MiSeq
sequencing method
14
days
sludge retention time
16S rRNA gene
target gene
Aerobic granular sludge
title
Synthetic wastewater
wastewater type