SAMN38137848
External Id
South China Agricultural University
INSDC center name
2024-09-15T00:50:21.743Z
INSDC first public
2024-09-15T00:50:21.743Z
INSDC last update
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
ERS22920967
SRA accession
2021-01
collection_date
B73
cultivar
Transcript end maturation is important for organellar gene expression, and mapping of RNA termini is a key step to unravel the underlying mechanism. However, limitation of current RNA mapping methods impedes the molecular study of organellar transcript end maturation in plants. Here, we take inspiration from circular RNA (circRNA), and establish the high-throughput transcript end mapping system (Hitens) together RNA 5-polyphsophatase treatment, in vitro RNA circularization, circRNA sequencing and the circRNA identification algorithm MeCi. With Hitens, the full sequences of 5,751 and 76,789 transcripts are determined in maize plastid and mitochondrion, respectively, corresponding to 1,162 and 13,757 high-confidence termini. Using these termini, 55 mature mRNAs, 10 precursor mRNAs, 31 intron splicing products, and 64 non-coding RNAs are identified in maize organelles, and these RNAs are experimentally verified. ZmRNaseII is dually targeted to plastid and mitochondrion, and the Hitens results show it is required for 3-end formation of nearly all mRNAs in plastid but only atp1 and atp4 mRNAs in mitochondrion.
description
Etiolated seedlings cultured at 15C
dev_stage
China: Guangzhou
geo_loc_name
Zea mays subsp. mays
http://purl.obolibrary.org/obo/NCBITaxon_381124
organism
mays
sub_species
Leaf
tissue
Development of high-throughput transcript end mapping system and functional study of ZmRNaseII in maize
title