SAMN38301931
External Id
Institute of Molecular Biology, Mainz
INSDC center name
2024-04-03T00:00:00Z
INSDC first public
2024-04-03T07:39:19.462Z
INSDC last update
SRS19568937
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS19568937
SRA accession
HeLa
cell line
derived from cervical cancer cells
cell type
missing
collection_date
WT
genotype
missing
geo_loc_name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
derived from cervical cancer cells
source_name
derived from cervical cancer cells
tissue
helawt_blunt_1
title
For lentiviral packaging of gRNA-target libraries, the blunt or staggered library was independently co-transfected with the two packaging plasmids and the supernatants were pooled and concentrated 50-100-fold. Packaging and transduction were performed. Briefly, we produced the viruses by co-transfection of 293T cells with each of the four library pools and two helper plasmids, psPAx2 and pMD2.g encoding the VSV-G envelope and the lentiviral gag-pol genes, respectively. We harvested the lentiviral vector-containing supernatant twice, at ~42 and 66 hours post-transfection and concentrated it by using Lenti-X-concentrator (Takara, 631232).
treatment