2025-01-16
ENA first public
ERC000023
ENA-CHECKLIST
SAMEA112329185
External Id
NETHERLANDS INSTITUTE OF ECOLOGY
INSDC center name
2023-01-16T12:43:30Z
INSDC last update
public
INSDC status
ERS14440095
SRA accession
PSBR_Day299_18S
Submitter Id
Photobioreactor
broad-scale environmental context
2020-06-30
collection date
Photogranules used for microscale functional analyses
description
Water
environmental medium
Netherlands
geographic location (country and/or sea)
51.98699968
DD
geographic location (latitude)
5.67116169
DD
geographic location (longitude)
The Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Gelderland
geographic location (region and locality)
Granulated biomass, photogranule
local environmental context
PCR
nucleic acid amplification
Qiagen DNAeasy Power Soil Pro
nucleic acid extraction
1
number of replicons
biofilm metagenome
organism
aerobic
oxygenation status of sample
6.8
pH
616*F-1132R
pcr primers
Local Treatment of Urban sewage and Streams for Healthy Reuse (LOTUSHR) - www.lotushr.org
project name
Photobioreactor operated in sequencing batch mode
reactor type
15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
sample material processing
0.2
g
sample volume or weight for DNA extraction
biofilm metagenome
scientific_name
Illumnia MiSeq
sequencing method
7
days
sludge retention time
18S rRNA gene
target gene
Photogranules
title
Synthetic wastewater
wastewater type