SAMN38288374
External Id
Nanfan Research Institute, Hainan University
INSDC center name
2024-07-24T00:50:18.157Z
INSDC first public
2024-07-24T00:50:18.157Z
INSDC last update
SRS19575944
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS19575944
SRA accession
50
age
Hai Lan,Maize Researh Insititute, Sichuan Agricultural University
biomaterial_provider
Chao Xia
collected_by
2023-03-05
collection_date
For this transcriptome study, various tissue were dissected from mature plants of ZnC442 × SCML0849 hybrid then stored in liguid nitrogen. Experiments were performed in three biological replicates each tissue consisting of 5 plants resulting in 27 distinct samples (9 tissue x 3 replicates). The total RNA of captured cells was isolated with trizol agent (Invitrogen). RNA quality was assessed via a Bioanalyzer (Agilent RNA 6000 Nano Chip, Agilent Technologies, Santa Clara, USA). For all samples, a RIN ≥ 6.5 detected. cDNA libraries for Illumina sequencing were constructed according to the protocol of the manufacturer (TruSeq RNA Sample Preparation, Illumina, San Diego CA, USA). For sequencing, each library was indexed by an Illumina TruSeq Adapter in which no adapter was used more than twice. Indexed libraries were loaded onto a flow cell following an incomplete block design with four pooled libraries per lane. Cluster preparation and pair-end read sequencing were performed according to the manufacturer’s instructions (HiSeq 4001, Illumina, San Diego CA, USA).
description
kernel filling stage
dev_stage
China
ecotype
China:Lincang
geo_loc_name
Zea mays
http://purl.obolibrary.org/obo/NCBITaxon_4577
organism
Aerialroot
tissue
1
title