SAMN38927111
External Id
United States Environmental Protection Agency
INSDC center name
2024-10-19T00:50:21.977Z
INSDC first public
2024-10-19T00:50:21.977Z
INSDC last update
SRS19930896
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS19930896
SRA accession
5-6 dpf
age
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
biomaterial_provider
US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
birth_location
not applicable
breed
All larval fathead minnows (Pimephales promelas) used for testing were obtained from an on-site culture facility at the US EPA Great Lakes Toxicology and Ecology Division in Duluth, MN, USA. Fathead minnow embryos were collected from multiple crosses of at least three females in paired breeding. Fertilized embryos that were attached to polyvinyl chloride breeding tiles were transferred to a bath of aerated Lake Superior water and held at 25 °C. All embryos were fertilized within a two-hour window. At five-day post-fertilization, larvae that had hatched and were freely swimming were collected, pooled, and randomly sorted for use. All procedures involving larval fathead minnows were approved by an animal care and use committee in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 2011; US EPA Animal Care and Use Plan # Eco24-09-001).
breeding_method
Great Lakes Toxicology & Ecology Division
collected_by
2021
collection_date
not applicable
cultivar
larvae
dev_stage
not applicable
ecotype
24 h
exposure_duration
0.0005
extrapolated_conc
mg/L
extrapolated_conc_units
USA: Minnesota, Duluth
geo_loc_name
not applicable
isolate
-
measured_conc
mg/L
measured_conc_units
0.0035
nominal_conc
mg/L
nominal_conc_units
Pimephales promelas
http://purl.obolibrary.org/obo/NCBITaxon_90988
organism
plateB
plate_ID
S150
replicate_ID
Total RNA
sample_type
not determined
sex
-80 deg C
store_cond
GLTED-Duluth-wt
strain
whole body
tissue
Larval fathead minnows exposed for 24hr to Parathion.
title
parathion (DTXSID7021100)
treatment