2025-01-17
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115129660
External Id
CEA/Saclay
INSDC center name
2024-01-23T18:02:52Z
INSDC last update
public
INSDC status
ERS17831670
SRA accession
E-MTAB-13732:HIF2_MRC5-HIF2_Rep1
Submitter Id
ArrayExpress
broker name
MRC-5
cell_line
fibroblast
cell_type
not collected
collection date
human
common name
Protocols: Trypsin digestion of attached cells and binding of 100,000 cells to magnetic concanavalin beads for Cut & Tag Proliferating cells in DMEM + Gluta-Max + 10% fetal bovine serum HIF2A over-expression versus HIF2A endogenous expression (pBabe control) Phenol-chloroform extraction after Cut & Tag. AMPure after PCR amplification of libraries. PCR amplification with Illumina Nextera indexed adaptors
description
embryo stage
developmental stage
normal
disease
HIF2A over-expression
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
Homo sapiens
scientific_name
HIF2_MRC5-HIF2_Rep1
title