SAMN38768647
External Id
Curtin University
INSDC center name
2024-04-04T00:00:00Z
INSDC first public
2024-04-04T03:06:02.318Z
INSDC last update
SRS19836827
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS19836827
SRA accession
not applicable
age
not applicable
biomaterial_provider
HeLa
cell_line
not applicable
cell_subtype
epithelial
cell_type
not applicable
collection_date
not applicable
culture_collection
HeLa cells were cultured at 37C under humidified 95% air/5% CO2 in Dulbecco's modified Eagle's medium (DMEM) containing glucose (4.5 g/l), 1 mM sodium pyruvate, 2 mM glutamine and 10% fetal bovine serum (FBS). Cells were seeded at 60% confluence in 24-well plates and transfected after 24 h with 500 ng of DNA in OptiMEM media (Gibco, Life Technologies) using a 1:1 mix of two transfection reagents (Lipofectamine LTX Reagent with Plus Reagent, Invitrogen, and FuGene HD, Promega). 72 h after transfection the cells were trypsinized and the cell pellets lysed for DNA extraction using the KAPA Express Extract Kit, according to the manufacturers instructions (Sigma-Aldrich). Amplicons were generated using primers flanking the gRNA and incorporating Illumina adaptor sequences.
description
adult
dev_stage
Adenocarcinoma
disease
not applicable
disease_stage
not applicable
ethnicity
not applicable
geo_loc_name
not applicable
health_state
not applicable
isolate
not applicable
karyotype
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
not applicable
phenotype
not applicable
population
not applicable
race
cell culture
sample_type
female
sex
Uterus; Cervix
tissue
HeLa_HyperDriveCas9
title
HyperDriveCas9
treatment