2025-01-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112354306
External Id
Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine, University of Leeds, Leeds, UK
INSDC center name
2023-01-19T12:37:47Z
INSDC last update
public
INSDC status
ERS14465204
SRA accession
E-MTAB-12494:Sample 10
Submitter Id
13
week
age
ArrayExpress
broker name
Norway rat
common name
Protocols: The strain of rats used in the experiment was ZSF1 purchased from Charles River (https://www.criver.com/products-services/find-model/zsf1-rat?region=3671). Rats in HFpEF_CR group were treated with dietary caloric restriction. Extensor digitorum longus (EDL) muscle was collected and transferred to liquid nitrogen until RNA extraction. RNA extraction of the EDL was performed using the Trizol-chloroform-isopropanol method following Trizol reagent solution user guide (ThermoFisher Scientific, Cat No. AM9738). Novogene, Cambridge, United Kingdom, performed RNA sequencing (RNA-seq). The library preparation was constructed using the TruSeq Stranded mRNA kit (Illumina), following the manufacturer’s recommended protocol. The libraries were sequenced on the HiSeq 4000 platform with 150 bp paired-end strategy.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
extensor digitorum longus
organism part
right
sampling site
Rattus norvegicus
scientific_name
male
sex
ZSF1-Leprfa Leprcp/Crl
strain
Sample 10
title