SAMN46301586
External Id
Wisconsin State Laboratory of Hygiene
INSDC center name
2025-01-18T00:00:00Z
INSDC first public
2025-01-18T12:49:21.467Z
INSDC last update
SRS23824227
INSDC secondary accession
live
INSDC status
SARS-CoV-2: wastewater surveillance
NCBI submission model
SARS-CoV-2.wwsurv.1.0
NCBI submission package
SRS23824227
SRA accession
2024-12-18
collection_date
ceres nanotrap
concentration_method
bovine coronavirus
extraction_control
thermo magmax viral/pathogen nucleic acid isolation kit
extraction_method
USA: Wisconsin
geo_loc_name
wastewater
isolation_source
wastewater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_527639
organism
Wisconsin SARS-CoV-2 Wastewater Monitoring Network
project_name
public health surveillance community-level
purpose_of_ww_sampling
Identification of the diversity of mutations across the SARS-CoV-2 metagenome
purpose_of_ww_sequencing
Wisconsin State Laboratory of Hygiene
sequenced_by
SARS-CoV-2: wastewater surveillance sample from wastewater metagenome
title
pepper mild mottle virus
ww_endog_control_1
not provided
ww_endog_control_1_conc
not provided
ww_endog_control_1_protocol
not provided
ww_endog_control_1_units
not provided
ww_flow
>100000
ww_population
population of jurisdiction encompassing the wastewater service area
ww_population_source
not applicable
ww_primary_sludge_retention_time
Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 1 (Ceres) and extracted using MagMAX Viral kit (Thermo Fisher Scientific), both on a KingFisher Flex.
ww_processing_protocol
24
ww_sample_duration
raw wastewater
ww_sample_matrix
wastewater treatment plant
ww_sample_site
composite
ww_sample_type
WI
ww_surv_jurisdiction
695
ww_surv_system_sample_id
SARS-CoV-2
ww_surv_target_1
not provided
ww_surv_target_1_conc
not provided
ww_surv_target_1_conc_unit
N gene (N1)
ww_surv_target_1_gene
not provided
ww_surv_target_1_known_present
GT molecular assay quantified using QIAcuity dPCR 24W (Qiagen)
ww_surv_target_1_protocol
SARS-CoV-2
ww_surv_target_2
not provided
ww_surv_target_2_conc
not provided
ww_surv_target_2_conc_unit
N gene (N2)
ww_surv_target_2_gene
not provided
ww_surv_target_2_known_present
GT molecular assay quantified using QIAcuity dPCR 24W (Qiagen)
ww_surv_target_2_protocol