SAMD00647587
External Id
Osafune Laboratory, Kyoto University, Center for iPS Cell Research and Application (CiRA)
INSDC center name
2024-01-18T00:00:00Z
INSDC first public
2024-06-02T17:06:23.857Z
INSDC last update
DRS360167
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
DRS360167
SRA accession
not applicable
age
Shinya Yamanaka
biomaterial_provider
hiPS cell derived kidney organoids
cell_type
2023-08-29
collection_date
Cryopreserved and thawed NPC aggregates, day 11 IPCs and ND cells were dissociated into single cells by incubation with Accumax and gentle pipetting. The dissociated single cells were mixed, resuspended in the organoid medium and seeded into 96-well low cell-binding U-bottom plates at a density of 1.7*10^4 NPCs/well, 1.7*10^4 IPCs/well and 1.7*10^4 ND cells/well to form aggregates. After 48 h, the mixed aggregates were transferred to a 24-well plate and incubated with 20 uL of 50% Matrigel in the same medium. After solidification to some extent, 180 uL of the same medium was added. Then, the medium was replaced with half the volume of fresh basal medium every two days.
description
Japan
geo_loc_name
not applicable
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Kidney_organoid2
sample_name
not applicable
sex
not applicable
tissue
Kidney organoid, iPSC derived, rep2
title