characteristics.text: SAMN40212070
characteristics.characteristic: External Id
characteristics.text: National Yang Ming Chiao Tung Universit
characteristics.characteristic: INSDC center name
characteristics.text: 2024-05-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-05-07T10:00:03.602Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20667683
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20667683
characteristics.characteristic: SRA accession
characteristics.text: 2023-10-30
characteristics.characteristic: collection_date
characteristics.text: This study investigated sex-specific effects in a gain-of-function model to evaluate Nfil3 function in relation to high-fat diet (HFD)-induced metabolic dysfunction-associated steatotic liver disease (MASLD) and gut microbiota (GM)-induced alterations in the bile acid (BA) profile. MASLD was induced in both wild type and Nfil3-deficient (NKO) C57BL/6J mice through an HFD. The hepatic immune response was evaluated using flow cytometry, revealing that NKO mice exhibited lower body weight, serum triglyceride (TG) levels, tissue injury, inflammation, and fat accumulation. The Nfil3 deletion reduced macrophage counts in fibrotic liver tissues, decreased proinflammatory gene and protein expression, and diminished gut barrier function. Alpha and beta diversity analysis revealed increased GM alpha diversity across different sexes. The Nfil3 gene deletion modified the BA profile, suggesting that negative feedback through the Nfil3-FXR-FGF15 axis facilitates BA recycling from the liver via enterohepatic circulation. Therefore, inhibiting Nfil3 in the liver offers a viable treatment approach for MASLD.
characteristics.characteristic: description
characteristics.text: Taiwan:Taipei
characteristics.characteristic: geo_loc_name
characteristics.text: mouse
characteristics.characteristic: host
characteristics.text: stool DNA
characteristics.characteristic: isolation_source
characteristics.text: 24.47 N 120.59 E
characteristics.characteristic: lat_lon
characteristics.text: mouse gut metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410661
characteristics.characteristic: organism
characteristics.text: Metagenome or environmental sample from Mouse gut metageome
characteristics.characteristic: title