characteristics.text: 0.69
characteristics.characteristic: Ammonium (µM)
characteristics.text: 4.213
characteristics.characteristic: Chlorophyll a (µg.L-1)
characteristics.text: biofilm
characteristics.characteristic: Compartment
characteristics.text: ENR
characteristics.characteristic: Condition
characteristics.text: 2021-03-09
characteristics.characteristic: Date
characteristics.text: SAMN40161018
characteristics.characteristic: External Id
characteristics.text: IFREMER
characteristics.characteristic: INSDC center name
characteristics.text: 2024-04-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-20T10:03:14.562Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20617289
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 2718.7
characteristics.characteristic: Nitrates (µM)
characteristics.text: 6.58
characteristics.characteristic: Nitrites (µM)
characteristics.text: 29.8
characteristics.characteristic: Phosphates (µM)
characteristics.text: SRS20617289
characteristics.characteristic: SRA accession
characteristics.text: D5
characteristics.characteristic: Sampling Day
characteristics.text: 0.89
characteristics.characteristic: Silicates (µM)
characteristics.text: 18S rRNA gene
characteristics.characteristic: Targeted gene
characteristics.text: 10
characteristics.characteristic: Temperature (°C)
characteristics.text: 2021-03-09
characteristics.characteristic: collection_date
characteristics.text: DNA extracted from the biofilm of 132.6 cm2 of Ulva thalli from site B and ENR raceway. The V4 variable region of the 18S rRNA gene was targeted using the TAReuk454FWD1 (CCAGCASCYGCGGTAATTCC) forward primer and the TAReukREV3 (ACTTTCGTTCTTGATYRA) reverse primer (Stoeck et al., 2010). Amplicons were obtained by PCR with 25 µL of reactional mix prepared with HotStarTaq polymerase (Qiagen, Hilden, Germany), 33 cycles and a melting temperature of 52 °C.
characteristics.characteristic: description
characteristics.text: France: Marine experimental platform from the French National Institute for Ocean Science or Institut Francais de Recherche pour l Exploitation de la Mer IFREMER
characteristics.characteristic: geo_loc_name
characteristics.text: Ulva
characteristics.characteristic: host
characteristics.text: Ulva spp. cultivation raceway with Mediterranean seawater + nitrate-based fertilizer
characteristics.characteristic: isolation_source
characteristics.text: 43.52 N 3.9 E
characteristics.characteristic: lat_lon
characteristics.text: biofilm metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_718308
characteristics.characteristic: organism
characteristics.text: 8.5
characteristics.characteristic: pH
characteristics.text: Ulva spp. biofilm
characteristics.characteristic: samp_collect_device
characteristics.text: DNA was extracted using a modified version of the Macherey Nagel NucleoSpin® plant II kit as in Thomas et al 2020 https://doi.org/10.1111/1758-2229.12806 and Brunet al 2021 https://doi.org/10.1111/1462-2920.15389
characteristics.characteristic: samp_mat_process
characteristics.text: 132.6 cm2 of Ulva spp. Thallus
characteristics.characteristic: samp_size
characteristics.text: 2021-03-09_ulva_biofilm_B_18S
characteristics.characteristic: source_material_id
characteristics.text: MI.M06549_0045.001.FLD_ill_042_i7---IDT_i5_5.210309_ENR_B_ATAReuk
characteristics.characteristic: title