characteristics.text: SAMN40267896
characteristics.characteristic: External Id
characteristics.text: National Institute of Allergy and Infectious Diseases
characteristics.characteristic: INSDC center name
characteristics.text: 2024-03-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-03-07T14:30:03.121Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20662947
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Invertebrate
characteristics.characteristic: NCBI submission model
characteristics.text: Invertebrate.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20662947
characteristics.characteristic: SRA accession
characteristics.text: 2023
characteristics.characteristic: collection_date
characteristics.text: Intact midguts (MG) from partially fed Amblyomma americanum adult females feeding on rabbits were collected at different stages during the blood feeding and stored in RNAlater solution. Ticks were grouped by weight, the average weight of the group 5 is 373.9 mg. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications. .
characteristics.characteristic: description
characteristics.text: Adult
characteristics.characteristic: dev_stage
characteristics.text: USA: Oklahoma
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: Ticks were from a pathogen specific-free colony of Amblyomma americanum maintained at the tick rearing facility at the Okhlahoma State University since 1976. The colony was started with engorged females collected from the natural population of A. americanum on the Cookson Game Refuge, located 5 miles east of Cookson, Oklahoma, Cherokee County.
characteristics.characteristic: isolate
characteristics.text: laboratory colony
characteristics.characteristic: isolation_source
characteristics.text: Amblyomma americanum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6943
characteristics.characteristic: organism
characteristics.text: Amblyomma americanum group 5 replicate 3
characteristics.characteristic: replicate
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Midgut
characteristics.characteristic: tissue
characteristics.text: Amblyomma americanum adult female midguts group 5 replicate 3
characteristics.characteristic: title