SAMN40526331
External Id
Sun Yat-Sen University
INSDC center name
2024-03-18T00:00:00Z
INSDC first public
2024-03-18T10:21:04.901Z
INSDC last update
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
ERS18587769
SRA accession
not collected
altitude
not collected
biomaterial_provider
not collected
collected_by
not collected
collection_date
not collected
culture_collection
not collected
depth
Quorum sensing (QS) is a cell-to-cell communication mechanism mediated by small diffusible signaling molecules. Previous studies showed that RpfR, which contains RpfF interaction (FI)-Per/Arnt/Sim (PAS)-GGDEF-EAL domains, controls Burkholderia cenocepacia virulence as a cis-2-dodecenoic acid (BDSF) QS signal receptor. Here, we report that the fatty acyl-CoA ligase DsfR (BCAM2136), which efficiently catalyzes in vitro synthesis of lauryl-CoA and oleoyl-CoA from lauric acid (C12:0) and oleic acid (C18:1), respectively, acts as a global transcriptional regulator to control B. cenocepacia virulence by sensing BDSF. It was revealed that BDSF binds to DsfR with high affinity and enhances the binding of DsfR to the promoter DNA regions of target genes. The BDSF-binding site are distinct from the substrate binding site of DsfR. Furthermore, we demonstrate that the homolog of DsfR in B. lata, RS02960, binds to the target gene promoter, and perception of BDSF enhances the binding activity of RS02960. Together, these results provide insights into the evolved unusual functions of the fatty acyl-CoA ligase DsfR that control bacterial virulence as a response regulator of QS signal.
description
not collected
env_broad_scale
not collected
genotype
not collected
geo_loc_name
Homo sapiens
host
not collected
host_tissue_sampled
not collected
identified_by
not collected
lab_host
not collected
lat_lon
not collected
mating_type
Burkholderia cenocepacia
http://purl.obolibrary.org/obo/NCBITaxon_95486
organism
not collected
passage_history
not collected
samp_size
not collected
sample_type
not collected
serotype
not collected
serovar
not collected
specimen_voucher
H111
strain
not collected
temp
Microbe sample from Burkholderia cenocepacia
title