SAMN40495845
External Id
Fujian Agriculture and Forestry University
INSDC center name
2024-03-16T00:00:00Z
INSDC first public
2024-03-16T16:32:20.173Z
INSDC last update
SRS20768543
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS20768543
SRA accession
16-year-old
age
2023-05-23
collection_date
tolerant cultivars (TC), resistant cultivars (RC) and neutral cultivars (NC)
cultivar
Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, causes significant damage to Pinus forests worldwide. Pinus massoniana is the primary host for PWD in southern China, but its response to PWN remains largely unstudied. Previously, we observed PWD in a P. massoniana nursery that contained over 71 cultivars and identified two groups of PWN resistant cultivars through field phenotyping. Resistant cultivars (RC) exhibited very low PWN carrying amounts (PCA) and low mortality, while tolerant cultivars (TC) had high PCA but low mortality. In this study, we confirmed that RC and TC had lower mortality rates of 10% and 11%, respectively, than other cultivars (which had a mortality rate of 83.3%). To explore the molecular mechanisms underlying the response of P. massoniana to PWN, the transcriptome and metabolome of these cultivars were profiled by high throughput sequencing. Furthermore, we generated a new full-length transcriptome library with iso-seq. Using the transcriptome and metabolome from different P. massoniana cultivars inoculated with PWN, we found three major PWD resistance strategies. 1) The common response strategy involved three important molecular pathways. First, synthesis of indole-3-acetic acid (IAA) and abcisic acid (ABA) were suppressed during PWN invasion, thus suppressing the synthesis of polysaccharides (especially myo-inositol and trehalose) and (-)-riboflavin through ABC transporters. Second, by inhibiting aspartic acid, the synthesis of arginine and proline through APS5 (aspartate aminotransferase 5) were suppressed. Third, by reducing cysteine, GERD (germacrene D synthase) was up-regulated, and sesquiterpenoid and triterpenoid metabolites were accumulated to resist PWN invasion. 2) The most effective resistance strategy involved the accumulation of reactive oxygen species (ROS), which enhanced jasmonic acid (JA) accumulation and highly induced the expression of chitinase, thus improving resistance to PWN. 3) The tolerance strategy involved the induction of phosphatidylcholine, which promoted flavonoid and anthocyanin synthesis via LPIN (phosphatidate phosphatase), LOX2S (lipoxygenase), and LOX1_5 (linoleate 9S-lipoxygenase), thus significantly inhibiting the pathogenicity of the PWN but not the PCA. These results illustrate the molecular mechanisms by which P. massoniana cultivars resist or tolerate PWN and are of great significance in the prevention and control of PWD.
description
China: Guanzhuang State-owned Forest Farm in Sanming, Fujian
geo_loc_name
Pinus massoniana
http://purl.obolibrary.org/obo/NCBITaxon_88730
organism
branch and trunk
tissue
The molecular basis of pine wilt disease resistance in Pinus massoniana
title