characteristics.text: SAMN40712054
characteristics.characteristic: External Id
characteristics.text: Northeast Agricultural University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-06-06T00:50:20.713Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-06T00:50:20.713Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS22978751
characteristics.characteristic: SRA accession
characteristics.text: E5-6 blastocyst
characteristics.characteristic: age
characteristics.text: large white pig
characteristics.characteristic: breed
characteristics.text: 2019-10
characteristics.characteristic: collection_date
characteristics.text: Extended pluripotent stem cells (EPSCs) are stem cells that derive from pre-implatation embryos in specific culture conditions. They can contribute to both embryonic (Em) and extraembryonic (ExEm) lineages. EPSCs derived from mouse and human embryos have been successfully established in the LCDM condition. However, the same has not yet been achieved with porcine embryos. To investigate the species-specific molecular mechanism of porcine embryo derived stem cells (pLCDM) in LCDM condition, we compared the biological characteristics of pLCDM and porcine extpanded potency stem cells (pEPSCs). The results revealed that pLCDM exhibited specific pluripotent traits and limited differentiation ability, with a strong inclination towards trophoblast lineage differentiation. These findings are significant and should be considered in future research endeavors. The objective of this study is to provide a comparative analysis of the pluripotency of pLCDM and pEPSC for a more comprehensive understanding. To comprehensively assess the pluripotency of pEPSC and pLCDM, low input RNA-seq was conducted on the cell lines. The transcriptome sequencing data of pLCDM (GSE197451) have been publicly published by this research team , while the transcriptome sequencing data of pEPSC established by our laboratory has not been uploaded, and this upload is the data upload of pEPSC.
characteristics.characteristic: description
characteristics.text: Passage 16
characteristics.characteristic: dev_stage
characteristics.text: China: Heilongjiang Province
characteristics.characteristic: geo_loc_name
characteristics.text: Sus scrofa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9823
characteristics.characteristic: organism
characteristics.text: not determined
characteristics.characteristic: sex
characteristics.text: embryonic-derived stem cell
characteristics.characteristic: tissue
characteristics.text: Porcine embryo-derived stem cell derived from porcine E5-6 blastocysts
characteristics.characteristic: title