characteristics.text: SAMN40711655
characteristics.characteristic: External Id
characteristics.text: Wisconsin State Laboratory of Hygiene
characteristics.characteristic: INSDC center name
characteristics.text: 2024-04-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-04-01T19:48:39.407Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20906528
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: SARS-CoV-2: wastewater surveillance
characteristics.characteristic: NCBI submission model
characteristics.text: SARS-CoV-2.wwsurv.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20906528
characteristics.characteristic: SRA accession
characteristics.text: 2024-03-20
characteristics.characteristic: collection_date
characteristics.text: ceres nanotrap
characteristics.characteristic: concentration_method
characteristics.text: bovine coronavirus
characteristics.characteristic: extraction_control
characteristics.text: promega ht tna kit
characteristics.characteristic: extraction_method
characteristics.text: USA: Wisconsin
characteristics.characteristic: geo_loc_name
characteristics.text: wastewater
characteristics.characteristic: isolation_source
characteristics.text: wastewater metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_527639
characteristics.characteristic: organism
characteristics.text: Wisconsin SARS-CoV-2 Wastewater Monitoring Network
characteristics.characteristic: project_name
characteristics.text: public health surveillance community-level
characteristics.characteristic: purpose_of_ww_sampling
characteristics.text: Identification of the diversity of mutations across the SARS-CoV-2 metagenome
characteristics.characteristic: purpose_of_ww_sequencing
characteristics.text: Wisconsin State Laboroatory of Hygiene
characteristics.characteristic: sequenced_by
characteristics.text: SARS-CoV-2: wastewater surveillance sample from wastewater metagenome
characteristics.characteristic: title
characteristics.text: pepper mild mottle virus
characteristics.characteristic: ww_endog_control_1
characteristics.text: 84960000
characteristics.characteristic: ww_endog_control_1_conc
characteristics.text: IDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
characteristics.characteristic: ww_endog_control_1_protocol
characteristics.text: copies/L
characteristics.characteristic: ww_endog_control_1_units
characteristics.text: 4148811.3328
characteristics.characteristic: ww_flow
characteristics.text: 7.16
characteristics.characteristic: ww_ph
characteristics.text: <100000
characteristics.characteristic: ww_population
characteristics.text: population of jurisdiction encompassing the wastewater service area
characteristics.characteristic: ww_population_source
characteristics.text: not applicable
characteristics.characteristic: ww_primary_sludge_retention_time
characteristics.text: Internal control spiked in samples at receipt. Samples concentrated using Nanotrap ER2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
characteristics.characteristic: ww_processing_protocol
characteristics.text: 24
characteristics.characteristic: ww_sample_duration
characteristics.text: raw wastewater
characteristics.characteristic: ww_sample_matrix
characteristics.text: wastewater treatment plant
characteristics.characteristic: ww_sample_site
characteristics.text: composite
characteristics.characteristic: ww_sample_type
characteristics.text: WI
characteristics.characteristic: ww_surv_jurisdiction
characteristics.text: 726778001
characteristics.characteristic: ww_surv_system_sample_id
characteristics.text: SARS-CoV-2
characteristics.characteristic: ww_surv_target_1
characteristics.text: 160320
characteristics.characteristic: ww_surv_target_1_conc
characteristics.text: copies/L
characteristics.characteristic: ww_surv_target_1_conc_unit
characteristics.text: N gene (N1)
characteristics.characteristic: ww_surv_target_1_gene
characteristics.text: yes
characteristics.characteristic: ww_surv_target_1_known_present
characteristics.text: IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
characteristics.characteristic: ww_surv_target_1_protocol
characteristics.text: SARS-CoV-2
characteristics.characteristic: ww_surv_target_2
characteristics.text: 88960
characteristics.characteristic: ww_surv_target_2_conc
characteristics.text: copies/L
characteristics.characteristic: ww_surv_target_2_conc_unit
characteristics.text: N gene (N2)
characteristics.characteristic: ww_surv_target_2_gene
characteristics.text: yes
characteristics.characteristic: ww_surv_target_2_known_present
characteristics.text: IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
characteristics.characteristic: ww_surv_target_2_protocol