characteristics.text: SAMN40873848
characteristics.characteristic: External Id
characteristics.text: Washington University in St Louis
characteristics.characteristic: INSDC center name
characteristics.text: 2024-04-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-04-08T15:01:07.173Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20962762
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20962762
characteristics.characteristic: SRA accession
characteristics.text: 8 weeks
characteristics.characteristic: age
characteristics.text: 2022-02-10
characteristics.characteristic: collection_date
characteristics.text: Bone marrow cells from C57Bl/6 mice were lineage-depleted and retrovirally transduced with murine stem cell virus (MSCV) vectors containing an internal ribosome entry site (IRES) followed by a Thy1.1 cDNA to allow for the purification of transduced cells. These vectors either contained no insert ("empty vector"), WT PML::RARA cDNA with a V5 epitope tag on C-terminus (PR-V5 or PML::RARA_WT-V5), or a V5-tagged C88A mutant in the RARA domain of PML::RARA (C88A or PML::RARA_C88A-V5). Two days following the transduction transduced cells were purified with anti-Thy1.1 magnetic bead enrichment, treated with 1 mM diisopropyl fluorophosphate, and CUT&RUN was performed with anti-V5 or isotype control (IgG) antibodies.
characteristics.characteristic: description
characteristics.text: Wild-type
characteristics.characteristic: genotype
characteristics.text: USA
characteristics.characteristic: geo_loc_name
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57Bl/22
characteristics.characteristic: strain
characteristics.text: Lineage-depleted bone marrow
characteristics.characteristic: tissue
characteristics.text: PML::RARA_C88A-V5_anti-V5_CUTandRUN_replicate_1
characteristics.characteristic: title