characteristics.text: SAMN40909111
characteristics.characteristic: External Id
characteristics.text: IFREMER
characteristics.characteristic: INSDC center name
characteristics.text: 2024-07-27T00:50:14.247Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-07-27T00:50:14.247Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20975873
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20975873
characteristics.characteristic: SRA accession
characteristics.text: 2023-07-19
characteristics.characteristic: collection_date
characteristics.text: France: Cylindrotheca closterium AC170 from Algobank Caen Collection, University of Caen Basse-Normandie, originally isolated from La Tremblade in 1991
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: Culture biofilm of Cylindrotheca closterium - Tank biofilm
characteristics.characteristic: isolation_source
characteristics.text: 45.46 N 1.16 W
characteristics.characteristic: lat_lon
characteristics.text: biofilm metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_718308
characteristics.characteristic: organism
characteristics.text: Cylindrotheca closterium was cultivated as a biofilm using a small rotating experimental culture system under controlled laboratory conditions (controlled temperature of 20°C and light of 80-90 µmol photons.m-2.s-1) with weekly sterile culture medium addition (modified f/2 medium with twice the concentration in nitrogen, phosphate and silicate, using synthetic sea salts Instant Ocean adjusted to reach a salinity of 40 g.L-1). A plastic tank (L35 cm x W25 cm x H15 cm) was used and contained a roller with a length of 30 cm and a diameter of 10 cm, with a rotating bar allowing for tangential velocity ranging from 0.02 to 0.07 m.s-1. A textile fabric was autoclaved and placed on the roller using zip ties. The fabric was moistened on the roller with a sterile culture medium for one day and inoculated by evenly pouring onto the fabric the planktonic culture of C. closterium at 5.7 rotations per minute (rpm) under a light intensity of 90 µmol photons m-2.s-1. The plastic tank was then half-filled with 3.6 L of sterile culture medium, ensuring that ¾ of the roller was exposed to the air while ¼ was immerged in the culture medium.
characteristics.characteristic: samp_mat_process
characteristics.text: Pbex16S-19F-Lucie_CGTTTC-L9VDC
characteristics.characteristic: title