characteristics.text: SAMN40871953
characteristics.characteristic: External Id
characteristics.text: Astrobiology Center Inta
characteristics.characteristic: INSDC center name
characteristics.text: 2024-04-08T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-04-10T12:47:59.302Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS18923089
characteristics.characteristic: SRA accession
characteristics.text: 2023-03
characteristics.characteristic: collection_date
characteristics.text: Illumina sequencing was applied to plastic and soil samples named P and S respectively. Purified DNA was quantified and 1ng of input DNA was used in a first PCR of 20 cycles with Q5 Hot Start High-Fidelity DNA Polymerase in the presence of 100nM primers for 16S and 18S amplification. After the first PCR, a second PCR of 15 cycles was performed with Q5 Hot Start High-Fidelity DNA Polymerase in the presence of 400nM of primers of the Access Array Barcode Library for Illumina Sequencers. The finally obtained amplicons were validated and quantified by Bioanalyzer and an equimolecular pool was purified using AMPure beads and titrated by quantitative PCR using the Kapa-SYBR FAST qPCR kit for LightCycler 480 and a reference standard for quantification. The pool of amplicons were denatured prior to be seeded on a flowcell at a density of 10pM, where clusters were formed and sequenced using a MiSeq Reagent Kit v3, in a 2x300 pair-end sequencing run on a MiSeq sequencer to obtain 100000 reads per sample approximately. Genomic data were analyzed with Base Space platform.
characteristics.characteristic: description
characteristics.text: Iceland
characteristics.characteristic: geo_loc_name
characteristics.text: Plastic and soil metagenomes
characteristics.characteristic: isolation_source
characteristics.text: 65.6835306 N 18.08779999 W
characteristics.characteristic: lat_lon
characteristics.text: soil metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410658
characteristics.characteristic: organism
characteristics.text: P_18S_S154_L001_R2_001
characteristics.characteristic: title