characteristics.text: 2025-01-27
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22719683
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14731:7_HepG2_125a1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HepG2
characteristics.characteristic: cell_line
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human liver cancer cell lines HepG2 and HuH-7 were cultured in RPMI 1640 and DMEM, respectively, containing 10% fetal bovine serum, 2 mM L-glutamine, 50 U/mL penicillin, and 100 mg/mL streptomycin. The day before transfection, cells were trypsinized and seeded in medium without antibiotics in 12-well plates. Transfections were then performed with cells at 80–90% of confluence by using 3 mL of Lipofectamine2000 (Thermo Fisher Scientific) for 1 mg of nucleic acids, as described by the manufacturer. In particular, miR-125a mimic or its control (Ctrl-miRNA) with unrelated sequence (all from Dharmacon) were transfected at 50 nM; 0.05 mg or 0.2 mg of reporter constructs were used for transfecting HuH-7 or HepG2 cells, respectively. The transfection mix was replaced with complete medium after 6 h, and the analyses were performed 48 h after transfection.  Total RNA was extracted from cell cultures using the miRNeasy mini kit (Qiagen, Hilden, Germany). The yield and quality of RNA were evaluated by NanoDrop One spectrophotometer (NanoDrop), Qubit 4 Fluorometer (Invitrogen) and by TapeStation 4200 (Agilent Technologies). Indexed libraries were prepared from 100 ng of each purified RNA sample using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero™ (Illumina), according to the manufacturer’s instructions. Libraries were quantified with the TapeStation 4200 (Agilent Technologies) and Qubit fluorometer (Invitrogen Co.). Libraries were pooled such that each index-tagged sample was present in equimolar amount.
characteristics.characteristic: description
characteristics.text: hepatocellular carcinoma
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 7_HepG2_125a1
characteristics.characteristic: title