35
Efflux Pumps Inactivated
SAMN41008237
External Id
Yes (FabR V42G)
FabR functional
University of Guelph
INSDC center name
2024-04-18T00:00:00Z
INSDC first public
2024-05-06T10:01:43.590Z
INSDC last update
SRS21215218
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
Yes
O-antigen production
No
Pore
SRS21215218
SRA accession
-
collection_date
To facilitate the study of drug efflux pump function, we previously reported the generation of EKO-35 (Efflux Knockout-35), a highly susceptible Escherichia coli K-12 (BW25113) mutant devoid of 35 known or putative drug efflux pumps (BioProject ID PRJNA838981). Here, we report the next-generation of this mutant, EKO-352.0. Efflux pump-encoding genes were disrupted using CRISPR-Cas9 mediated counterselection, producing a pan efflux-deficient mutant that harbours only one secondary nonsynonymous mutation compared to the parental strain. Additionally, E. coli K-12 laboratory-associated cell envelope mutations were repaired including restoration of the wbbL locus, enabling production of O-antigen, and the BW25113-associated fabR missense mutation.
description
-
geo_loc_name
-
host
-
isolate
-
isolation_source
Escherichia coli
http://purl.obolibrary.org/obo/NCBITaxon_562
organism
-
sample_type
K-12 BW25113
strain
EKO-35-2.0
title