SAMN41129559
External Id
Interactive Research School for Health Affairs-Bharati Vidyapeeth(Deemed to be University),Pune
INSDC center name
2024-07-29T00:50:23.867Z
INSDC first public
2024-07-29T00:50:23.867Z
INSDC last update
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
ERS22993310
SRA accession
1 year
age
Cell Repository, NCCS, Pune ,India
biomaterial_provider
THP-1
cell_line
Monocyte
cell_subtype
Myeloid
cell_type
Not collected
collection_date
Macrophage polarization is a pivotal phenomenon influencing all stages of cancer progression. Particularly, Tumor-associated macrophages (TAMs) are classified into M1 (anti-tumoral) and M2 (pro-tumoral) macrophages. Within the M2 category, subtypes M2a and M2d are recognized as significant models for TAMs, with M2d macrophages closely resembling TAMs in vitro. Herein, we induced M0, M1, M2a, and M2d phenotypes in THP-1 cells, generating THP-1 derived macrophages. Comparative transcriptome analysis revealed distinct phenotypic signatures, with M2d macrophages exhibiting an upregulation of TAM-related marker genes at the mRNA level, distinguishing them from M2a macrophages. Our findings present a valuable resource for investigating M2d macrophages, providing a nuanced in vitro model for TAMs in contrast to the well-established M2a macrophages. This comprehensive characterization will be helpful in quest of understanding of macrophage diversity and offers insights into their role in cancer progression.
description
India
geo_loc_name
1year Male child with Acute myeloid leukemia
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
male
sex
Blood
tissue
Human sample from Homo sapiens
title