characteristics.text: SAMN41220828
characteristics.characteristic: External Id
characteristics.text: Institute of Zoology, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2024-05-06T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-05-06T02:01:11.380Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21213173
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21213173
characteristics.characteristic: SRA accession
characteristics.text: 6-8 weeks
characteristics.characteristic: age
characteristics.text: wt
characteristics.characteristic: breed
characteristics.text: 2022
characteristics.characteristic: collection_date
characteristics.text: wt
characteristics.characteristic: ecotype
characteristics.text: China
characteristics.characteristic: geo_loc_name
characteristics.text: SAMPLE9 Bone marrow was flushed from femurs and tibias of B6 mice. BMDMs were harvested in complete DMEM medium (DMEM supplemented with 1% penicillin/streptomycin and 5% FBS) containing GM-CSF (50 ng/mL) for 7 days. On day 3 and day 7, non-adherent cells were discarded, and then fresh GM-CSF-containing complete DMEM medium was added. For macrophage subset screening, a total of 22 cytokines or stimuli were used to treat sorted PEMs for 48 h in complete DMEM medium. For the M1 subset induction, macrophages were treated with 100 ng/mL LPS for 6 h, the M2 subset was induced by adding IL-4 (10 ng/mL) for 48 h, and the M17 subset were obtained by treatment with IL-23 (10 ng/mL) for 48 h 20. We defined 75 ng/mL IL-33-treated macrophages as the M(IL-33) subset and the M(IL-33+IL-2) subset were induced by IL-33 (75 ng/mL) and IL-2 (10 ng/mL) for 48 h. The untreated macrophage was M0.
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: not collected
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: bone marrow
characteristics.characteristic: tissue
characteristics.text: Model organism or animal sample from Mus musculus
characteristics.characteristic: title