characteristics.text: 2025-01-29
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Maastricht University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18825323
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13996:AN10_Liver_S10
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Norway rat
characteristics.characteristic: common name
characteristics.text: Protocols: Representative samples of blood, gross lesions, kidney, liver, thymus and uterus were collected from all animals and preserved in 10% neutral buffered formalin or snap frozen in liquid nitrogen and stored in deep freezer (<-75°C). Organs were collected at scheduled necropsy. The total RNA of all 96 samples were extracted using different methods depending on the tissue of origins. Solid tissues (Liver, Kidney and Thymus) were extracted using the miRNeasy Mini Kit (Qiagen, Cat.No. 217004). For this, small pieces of tissue were cut and dissolved in 700 µl of Qiazol using the MiniBeadBeaterPlus for two cycles of 15 seconds and 5 minutes, respectively. The blood was processed with the RNeasy Protect Animal Blood Kit, (Qiagen , Cat. No. 73224). All extracted total RNAs were then assessed for quality and dosed using a bioAnalyser tracted total RNA were first treated by Ribo-depletion using the NEXTFLEX Ribonaut rRNA Depletion. For this, 1ug of total were used for all solid tissue, and 143 ng for the blood (corresponding of the smallest extracted amount for animal 19). Library preparation was then made using the NEXTFLEX Rapid Directional RNA-Seq Kit 2.0, with the corresponding unique dual indexes
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Rattus norvegicus
characteristics.characteristic: organism
characteristics.text: Liver
characteristics.characteristic: organism part
characteristics.text: Rattus norvegicus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sprague Dawley rats
characteristics.characteristic: strain
characteristics.text: AN10_Liver_S10
characteristics.characteristic: title