SAMN41452624
External Id
Henan Agricultural University
INSDC center name
2024-05-19T00:00:00Z
INSDC first public
2024-05-19T23:01:05.673Z
INSDC last update
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
ERS20158173
SRA accession
2023-01-17
collection_date
Zhongmu NO.3
cultivar
Alfalfa (Medicago sativa L.) is widely cultivated and planted worldwide due to its high yield, good nutritional quality, and wide adaptability. Salt stress is one of the most important abiotic stresses that lead to reduced yield and growth inhibition in Alfalfa. Gibberellin (GA) is an endogenous hormone synthesized by plants. It is known that Gibberellin A3 (GA3) can improve the salt tolerance of Alfalfa by remodeling ion homeostasis, regulating osmotic pressure synthesis and activating antioxidant enzymes. In addition, GA3 can also regulate the synthesis and degradation of various bioactive molecules in plants and participate in the expression of stress related genes with the synergy and antagonism between GA3 and other plant hormones. Therefore, it plays an important role in plant stress resistance. However, the genetic and molecular mechanisms mediating the salt tolerance of Alfalfa still need to be better understood. In order to investigate the key genes and pathways mediating the effect of GA3 on alleviating salt stress, we conducted physiological and biochemical analysis and transcriptomic analysis on Alfalfa seedlings. The results showed that GA3 protects Alfalfa from salt stress by regulating the balance between Alfalfa growth and salt stress. Under salt stress, the content of Superoxide anion (O2-) in the sample decreased significantly, which was slightly restored after adding GA3. Superoxide dismutase (SOD) and peroxidase (POD) activities increased under salt stress treatment alone or combined with GA3 treatment. Salt stress significantly decreased the accumulation of Glutathione (GSH) and Malondialdehyde (MDA). But the accumulation was restored and increased after adding GA3. We then conducted transcriptomic analysis on the samples and compared the salt treatment group with the control group; we obtained 4206 differentially expressed genes (DEGs) in total, including 2088 up-regulated genes and 2118 down-regulated genes, while the addition of the GA3 group obtained 5352 DEGs, including 2706 up-regulated genes and 2646 down-regulated genes. Compared with salt stress treatment alone, there were 282 differentially expressed genes in the addition of the GA3 group, including 129 up-regulated genes and 153 down-regulated genes. Through GO functional annotation and KEGG pathway enrichment, we identified some candidate genes affecting salt tolerance, which were mainly enriched in the biosynthesis of flavonoids, plant hormones, glutathione (GSH), soluble sugars, and other substances, as well as ATP-binding cassette transporter (ABC transporters) and mitogen-activated protein kinase (MAPK) signaling pathways, as well as the circadian rhythm-plant pathway. This suggests that the mechanism of GA3 alleviating salt stress may be strongly associated with regulating the expression of genes related to energy supply and antioxidant capacity, which has important reference value for breeding salt-tolerant Alfalfa and other crops.
description
seedling stage
dev_stage
China:Zhengzhou
geo_loc_name
Medicago sativa
http://purl.obolibrary.org/obo/NCBITaxon_3879
organism
seedling
tissue
Plant sample from Medicago sativa
title