2025-01-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113542863
External Id
Associate Professor The Chemosensory Lab Department of Computational Biology Indraprastha Institute of Information Technology-Delhi (IIIT-Delhi)
INSDC center name
2023-05-23T15:44:46Z
INSDC last update
public
INSDC status
ERS15539280
SRA accession
E-MTAB-12992:NC_T2_U2
Submitter Id
ArrayExpress
broker name
baker's yeast
common name
Protocols: The yeast culture was treated and then centrifuged. Yeast cells (BY4741) were grown at 30°C at 200 r.p.m. in YPD medium. The cells were grown in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite loading, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours. RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing. the nucleic acid library was successfully prepared
description
wild type genotype
genotype
YPD medium at 30 degree Celsius
growth condition
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
organism
whole organism
organism part
Saccharomyces cerevisiae
scientific_name
BY4741
strain
NC_T2_U2
title