SAMN41415681
External Id
University of Parma
INSDC center name
2024-05-16T00:00:00Z
INSDC first public
2024-05-16T07:01:08.890Z
INSDC last update
SRS21314000
INSDC secondary accession
live
INSDC status
MIGS.ba
NCBI submission model
MIGS/MIMS/MIMARKS.human-gut
NCBI submission model
MIGS.ba.human-gut.6.0
NCBI submission package
SRS21314000
SRA accession
2023-12-02
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
not applicable
env_broad_scale
ENVO:01001033
env_local_scale
without Caco-2/HT29-MTX cell monolayers
env_medium
Italy
geo_loc_name
Homo sapiens
host
Cells were grown in MRS broth under anaerobic conditions at 37C until they reached the exponential growth phase (0.6 < OD600nm < 0.8). Cells were counted using a Thoma cell counting chamber (Herka), diluted to a final concentration of 1 108 cells/mL, washed in PBS, and resuspended in 400 L of antibiotic-free serum-supplemented DMEM.
isol_growth_condt
44.76816168 N 10.31368476 E
lat_lon
1
num_replicons
Bifidobacterium animalis
http://purl.obolibrary.org/obo/NCBITaxon_28025
organism
PMID: 36846784
ref_biomaterial
PRL2013
strain
Bifidobacterium animalis subsp. lactis PRL2013 without Caco-2/HT29-MTX cell monolayers
title