characteristics.text: SAMN41523180
characteristics.characteristic: External Id
characteristics.text: USDA-ARS-USMARC
characteristics.characteristic: INSDC center name
characteristics.text: 2024-10-03T12:39:41.433Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-03T12:39:41.433Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS.ba
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.ba.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: 2018-03-23
characteristics.characteristic: collection_date
characteristics.text: chemically defined minimal medium culture of SMOOTH colony sampleID 100192
characteristics.characteristic: collection_method
characteristics.text: A suspension of both SMOOTH and NONSMOOTH colonies (sample ID 100158) was used to inoculate a 72 hour anaerobic culture in chemically defined minmal medium in bioreactor vessel 1. This culture was diluted and plated on blood agar base + hemin medium and grown overnight at 37C under 5% CO2 to generate a SMOOTH colony with source material sampleID 100192. This colony was aerobically cultured overnight in chemically defined minmal medium from which the DNA for sequencing was extracted.
characteristics.characteristic: description
characteristics.text: SMOOTH phenotype
characteristics.characteristic: encoded_traits
characteristics.text: ENVO:00002123
characteristics.characteristic: env_broad_scale
characteristics.text: ENVO:00002124
characteristics.characteristic: env_local_scale
characteristics.text: MICRO:0000067
characteristics.characteristic: env_medium
characteristics.text: 2500000
characteristics.characteristic: estimated_size
characteristics.text: USA: Clay Center, NE
characteristics.characteristic: geo_loc_name
characteristics.text: Bos taurus
characteristics.characteristic: host
characteristics.text: Bovine Respiratory Disease Complex
characteristics.characteristic: host_disease
characteristics.text: 9913
characteristics.characteristic: host_taxid
characteristics.text: 5955799
characteristics.characteristic: isol_growth_condt
characteristics.text: Derived from an anaerobic bioreactor culture in chemically defined minimal medium (CDMM) inoculated with a CDMM suspension of both SMOOTH and NONSMOOTH colonies grown on blood agar base + hemin plate (BABH) medium. This inoculating CDMM suspension assigned sampleID 100158. These BABH colonies were generated from a both SMOOTH and NONSMOOTH colonies on chocolate agar inoculated from a glycerol stock with sampleID 184 (SAMN03275447)
characteristics.characteristic: isolation_source
characteristics.text: NA
characteristics.characteristic: lat_lon
characteristics.text: 1
characteristics.characteristic: num_replicons
characteristics.text: Mannheimia haemolytica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_75985
characteristics.characteristic: organism
characteristics.text: Bos taurus
characteristics.characteristic: pathogenicity
characteristics.text: NA
characteristics.characteristic: ref_biomaterial
characteristics.text: facultative
characteristics.characteristic: rel_to_oxygen
characteristics.text: 100192
characteristics.characteristic: source_material_id
characteristics.text: Mh184.20180323.PV.B
characteristics.characteristic: strain
characteristics.text: phase variant
characteristics.characteristic: subspecf_gen_lin
characteristics.text: Mannheimia haemolytica USDA-ARS-184 Phase Variant 20180323.B
characteristics.characteristic: title