characteristics.text: SAMN41551940
characteristics.characteristic: External Id
characteristics.text: Riken
characteristics.characteristic: INSDC center name
characteristics.text: 2024-05-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-05-31T04:08:02.483Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21437601
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21437601
characteristics.characteristic: SRA accession
characteristics.text: not applicable
characteristics.characteristic: age
characteristics.text: P0,P1,P2,AB,ABa
characteristics.characteristic: cell_type
characteristics.text: not applicable
characteristics.characteristic: collection_date
characteristics.text: 30 mature nematodes were collected and transferred into a solution consisting of 20 μL of egg buffer [42]. The nematodes' bodies were then cut with needles (TERMO, NN-2613S) to extract eggs, which were subsequently transferred a 1.5 mL glass tube (miyahara, mt1.5). Following this, 600 μL of egg buffer was added to the tube, and the contents were centrifuged at 850 ×g at 15°C for 15 s, after which the supernatant was removed. Subsequently, 100 μL of bleach solution (0.5% sodium hypochlorite solution (Wako, 197-02206) with 1% effective chlorine concentration, stored at -80°C) was added to remove the vitelline layer of eggs, and the tube was left at room temperature for 2 min. Following this, the tube was centrifuged again at 850 ×g at 15°C for 15 s, and the supernatant was removed. 200 μL of egg buffer was then added, followed by centrifugation at 850 ×g at 15°C for 15 s, with subsequent removal of the supernatant. Egg shell removal was achieved by adding 15 μL of a mixture consisting of chymotrypsin and chitinase (5 U chitinase (Sigma, C6137-5UN) and 10 mg α-chymotrypsin (Sigma, C4129-500MG) in 1 mL egg buffer), allowing it to incubate at room temperature for 5 min, followed by centrifugation at 850 ×g at 15°C for 15 s and removal of the supernatant. To weaken the permeable barrier of eggs, 200 μL of bleach solution was added, and the tube was incubated for 30 s before centrifugation at 850 ×g at 15°C for 20 s, and subsequent removal of the supernatant. The eggs were washed by repeating the addition of sucrose mixture medium and removal of the supernatant twice. Finally, 1 mL of sucrose mixture medium was added to the tube, and the medium containing the eggs was transferred to a 35 mm glass-bottom dish (IWAKI, 3911-035) treated with 0.1 mg/mL poly-L-lysine (Sigma-Aldrich, P8920-100ML) at room temperature for more than15 min, after which the dish was placed onto the stage of inverted microscope.
characteristics.characteristic: description
characteristics.text: 1-cell to 4-cell stage embryo
characteristics.characteristic: dev_stage
characteristics.text: N2
characteristics.characteristic: ecotype
characteristics.text: not applicable
characteristics.characteristic: geo_loc_name
characteristics.text: Caenorhabditis elegans
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_6239
characteristics.characteristic: organism
characteristics.text: Embryonic mortality
characteristics.characteristic: phenotype
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: oma-1(zu405)
characteristics.characteristic: strain
characteristics.text: early stage embryo
characteristics.characteristic: tissue
characteristics.text: C. elegans, live-organismRNA-seq
characteristics.characteristic: title