characteristics.text: 2025-01-31
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115031432
characteristics.characteristic: External Id
characteristics.text: Wellcome/Cancer Research UK Gurdon Institute University of Cambridge
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-14T16:51:34Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17478365
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13632_2:SB-early day 8
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: embryonic stem cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human ES cell colonies were dissociated into single cells using 0.25% Trypsin-EDTA, seeded (600,000 cells/well) into freshly prepared Vitronectin-coated 6-well plates and cultured for 24h in presence of Advanced RPMI 1640 supplemented with B27 supplement (1%), 0.1mM NEAA, 100U/ml Penicillin, 0.1 mg/ml Streptomycin, 2mM L-glutamine, Activin A (1µg/ml), Chiron (3µM) and Rho-associated, coiled-coil containing protein kinase (ROCK) inhibitor (ROCKi (10µM)). Transient cells induced for 24h were dissociated using 0.25% Trypsin-EDTA, and 480,000 cells were seeded into each well of an AggrewellTM 400 Plate at the rate of 400 cells/microwell and were centrifuged at 300 X g for 3 mins. Essential 6 (E6) medium supplemented with 0.25µM CH and ROCKi (10µM) was used for self-organization of the intermediate cells. The final volume of medium in each well was 2ml. Cells were cultured in Aggrewell plates at 37°C, at 5% CO2 for 4d in presence of OM. From D4 onwards, peEBs were transferred to rotary culture system with 30 revolution per minute (rpm) in presence of TGF-b inhibitor, SB43, for additional 10 days (Total 14 days). The culture medium (without ROCKi) was replaced every day. Samples were collected at D8 and D14 for sequencing. Chromium Single Cell 3’ v3, 10X Genomics Chromium Single Cell 3’ v3, 10X Genomics
characteristics.characteristic: description
characteristics.text: embryo
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: inner cell mass
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: SB-early day 8
characteristics.characteristic: title