characteristics.text: 2025-01-31
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Physiology and Pharmacology Karolinska Institutet
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22551403
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14665:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: cancer cell line
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were sacrificed when at least one tumor had reached the maximum allowed size; tumors were then separated from the skin. RNA was extracted from LAMP2A WT and KO tumors from BALB/c nude mice with PureLink RNA Mini Kit and determined with the RNA 6000 Nano Kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
characteristics.characteristic: description
characteristics.text: fibrosarcoma
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 3
characteristics.characteristic: title
characteristics.text: LAMP2A KO cell line-derived xenograft
characteristics.characteristic: xenograft