characteristics.text: SAMN41551938
characteristics.characteristic: External Id
characteristics.text: Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2024-05-27T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-05-28T00:18:20.657Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21443925
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Pathogen.cl
characteristics.characteristic: NCBI submission model
characteristics.text: Pathogen.cl.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21443925
characteristics.characteristic: SRA accession
characteristics.text: Southern University of Science and Technology Hospital
characteristics.characteristic: collected_by
characteristics.text: 2023-03-31
characteristics.characteristic: collection_date
characteristics.text: In recent years, with the increasing global antimicrobial resistance, phage therapy has emerged as one of the most promising alternatives to antibiotics. Numerous studies have reported the effectiveness and safety of phage therapy in treating multidrug-resistant bacterial infections. However, there is limited research on the relationship between the emergence of phage-resistant strains and clinical outcomes. In this study, we applied phage nebulization therapy to a patient with carbapenem-resistant Pseudomonas aeruginosa lung infection. After 8 weeks of treatment with P. aeruginosa phages, the patient's sputum bacterial load decreased, overall inflammatory markers decreased, and lung effusion significantly reduced. During the treatment, a phage-resistant P. aeruginosa strain NPA13B was isolated. To investigate the relationship between the emergence of phage-resistant strains and clinical outcomes, we performed whole-genome sequencing analysis on the resistant strain and validated the effect of phage resistance on bacterial virulence using the Galleria mellonella larvae infection model. Our conclusion is that phage therapy against P. aeruginosa demonstrates positive effects in reducing the bacterial load and improving clinical symptoms in the lungs.
characteristics.characteristic: description
characteristics.text: China: Shenzhen
characteristics.characteristic: geo_loc_name
characteristics.text: Homo sapiens
characteristics.characteristic: host
characteristics.text: Pneumonia due to Pseudomonas
characteristics.characteristic: host_disease
characteristics.text: Sputums of an 83-year-old male patient.
characteristics.characteristic: isolate
characteristics.text: Sputums
characteristics.characteristic: isolation_source
characteristics.text: 22.58 N 113.95 E
characteristics.characteristic: lat_lon
characteristics.text: Pseudomonas aeruginosa
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_287
characteristics.characteristic: organism
characteristics.text: Pseudomonas aeruginosa isolated from human sputums
characteristics.characteristic: title