characteristics.text: Eukaryotic
characteristics.characteristic: Breed
characteristics.text: SAMN41486083
characteristics.characteristic: External Id
characteristics.text: George Washington University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-08-18T00:50:15.353Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-18T00:50:15.353Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: U-87MG Cells
characteristics.characteristic: Label
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS23017681
characteristics.characteristic: SRA accession
characteristics.text: U-87MG Cells
characteristics.characteristic: Strain
characteristics.text: NA
characteristics.characteristic: age
characteristics.text: ATCC
characteristics.characteristic: biomaterial_provider
characteristics.text: U-87 MG
characteristics.characteristic: cell_line
characteristics.text: Brain
characteristics.characteristic: cell_type
characteristics.text: 2019-10-02
characteristics.characteristic: collection_date
characteristics.text: Unlocking the intricate mechanisms underlying diseases like cancer requires a comprehensive understanding of gene expression patterns. In this study, we employed high throughput RNA sequencing (RNA-Seq) to unravel the transcriptomic landscape of U-87 MG cells, a commonly used glioblastoma cell line. Obtained from the American Type Culture Collection (ATCC), U-87 MG cells serve as a valuable model for studying glioblastoma, a highly aggressive form of brain cancer. Our investigation focused on deciphering the effects of Cold Atmospheric Plasma (CAP) treatment on U-87 MG cells compared to untreated control samples. CAP, a novel therapeutic approach, has shown promising anti-cancer properties by inducing reactive oxygen and nitrogen species (RONS) in the tumor microenvironment. By subjecting U-87 MG cells to CAP exposure for 1 minute and varying incubation time (ranging from 1, 24, and 48 hours) and in combination with Temozolomide (TMZ), a standard chemotherapy drug, we aimed to elucidate the dynamic changes in gene expression profiles. The experimental design encompassed multiple time points post-treatment, enabling a comprehensive assessment of gene expression alterations induced by CAP and TMZ. RNA-Seq facilitated the quantification of gene expression levels, allowing us to identify differentially expressed genes (DEGs) in response to treatment. Notably, comparisons between CAP-treated samples and controls revealed significant changes in gene expression patterns, indicative of cellular responses to CAP-induced stress. Furthermore, the incorporation of CAP exposure as well as the combination treatment with TMZ, provided insights into the temporal dynamics and potential synergistic effects of the therapeutic interventions. Through rigorous bioinformatic analysis, we delineated key molecular pathways and biological processes modulated by CAP and TMZ treatment, shedding light on their mechanisms of action in glioblastoma cells. Overall, our study contributes to advancing the understanding of glioblastoma pathophysiology and therapeutic strategies. The comprehensive transcriptomic profiling of U-87 MG cells post-CAP and TMZ treatment offers valuable insights into the molecular mechanisms underlying cancer progression and response to treatment. This knowledge may inform the development of novel therapeutic approaches and personalized treatment strategies for glioblastoma patients, ultimately improving clinical outcomes and patient care.
characteristics.characteristic: description
characteristics.text: Malignant glioblastoma
characteristics.characteristic: disease
characteristics.text: USA: DC
characteristics.characteristic: geo_loc_name
characteristics.text: U-87 MG cells
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Brain cells
characteristics.characteristic: tissue
characteristics.text: Transcriptomic Profiling of U-87 MG Cells (HTB-14) from ATCC Using RNA Sequencing
characteristics.characteristic: title