characteristics.text: SAMN41489809
characteristics.characteristic: External Id
characteristics.text: University of Edinburgh
characteristics.characteristic: INSDC center name
characteristics.text: 2024-11-01T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-11-01T07:42:02.338Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21397742
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21397742
characteristics.characteristic: SRA accession
characteristics.text: 2024-05-22
characteristics.characteristic: collection_date
characteristics.text: Raw RNA sequencing data from five clones of pleomorphic T. brucei EATRO 1125 AnTat 1.1 90:13. The clones were grown in HMI-9 at 37oC and 5 percent CO2 for a total of 72 days. The HMI-9 media was supplemented with BHI. During the 72-day selection, the percentage of BHI was progressively increased from 2.5 percent to 15 percent. The cell density was counted at each passage using a Beckman Z2 Coulter particle counter and size analyser. High cell density was deliberately maintained to select proliferating cells at high parasite population density. RNA was extracted from triplicate cultures of the 'start' and 'end' from the five clones described above. The cells were grown to a density of between 7.5x10*5 cells/ml and 1x10*6 cells/ml and RNA was then extracted using the Qiagen RNeasy Mini kit, following the manufacturer's instructions. RNA was then sequenced with DNBseq by BGI (Hong Kong).
characteristics.characteristic: description
characteristics.text: United Kingdom
characteristics.characteristic: geo_loc_name
characteristics.text: in vitro culture media
characteristics.characteristic: isolation_source
characteristics.text: Trypanosoma brucei brucei
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5702
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: EATRO 1125 AnTat 1.1 90:13
characteristics.characteristic: strain
characteristics.text: brucei
characteristics.characteristic: sub_species
characteristics.text: Transcriptome sequencing from clonal T. brucei selected for monomorphism
characteristics.characteristic: title