characteristics.text: SAMN41656364
characteristics.characteristic: External Id
characteristics.text: Harvard University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-06-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-03T15:25:25.810Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21502512
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21502512
characteristics.characteristic: SRA accession
characteristics.text: 7 days post-differentiation
characteristics.characteristic: age
characteristics.text: George Church; 3 Blackfan Circle, Boston MA 02124
characteristics.characteristic: biomaterial_provider
characteristics.text: Differentiated human induced pluripotent stem cells
characteristics.characteristic: cell_type
characteristics.text: 2023-08-03
characteristics.characteristic: collection_date
characteristics.text: Cells containing integrated expression vectors were seeded in mTeSR1 containing 10 µM Y-27632, 5 µM GSK3484862, and 1 µg/mL doxycycline. After 24 hours, the medium was changed to APEL2 with 5 µM GSK3484862, 1 µM AM580, and 1 µg/mL doxycycline. A media change was performed every other day. After a total of seven days of differentiation, cells were harvested with Accutase and sorted based on reporter expression. Cells were fixed using a Parse fixation kit, and scRNAseq library preparation was performed using a Parse WT Mega v2 kit. A list and description of samples is provided in Supplementary Table 6.
characteristics.characteristic: description
characteristics.text: USA
characteristics.characteristic: geo_loc_name
characteristics.text: Differentiated human induced pluripotent stem cells, sublibrary 10
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: pooled male and female
characteristics.characteristic: sex
characteristics.text: Differentiated human induced pluripotent stem cells
characteristics.characteristic: tissue
characteristics.text: Meiosis_screening_sublibrary_10
characteristics.characteristic: title