characteristics.text: SAMN41656375
characteristics.characteristic: External Id
characteristics.text: Harvard University
characteristics.characteristic: INSDC center name
characteristics.text: 2024-06-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-06-03T15:25:24.107Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21502501
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21502501
characteristics.characteristic: SRA accession
characteristics.text: 7 days post-differentiation
characteristics.characteristic: age
characteristics.text: George Church; 3 Blackfan Circle, Boston MA 02122
characteristics.characteristic: biomaterial_provider
characteristics.text: Differentiated human induced pluripotent stem cells
characteristics.characteristic: cell_type
characteristics.text: 2023-09-15
characteristics.characteristic: collection_date
characteristics.text: To enrich the library for barcode sequences, we first performed PCR with biotinylated primers to generate a dsDNA biotinylated bait containing the 120bp of sequence immediately 3’ of the barcode sequence in our expression vector. We isolated the bait DNA from the PCR using a 3X volume of ProNex beads, and eluted in 10 mM Tris pH 8.0 buffer. Next, we used 200 fmol of bait DNA as a custom probe in the Parse Gene Capture kit, following the manufacturer’s protocol aside from this substitution. After qPCR to verify the barcode enrichment, the resulting library was sequenced on one lane of a NovaSeq X Plus 10B PE150 flowcell.
characteristics.characteristic: description
characteristics.text: USA
characteristics.characteristic: geo_loc_name
characteristics.text: Differentiated human induced pluripotent stem cells, barcode capture 8
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: pooled male and female
characteristics.characteristic: sex
characteristics.text: Differentiated human induced pluripotent stem cells
characteristics.characteristic: tissue
characteristics.text: Barcode_capture_sublibrary_08
characteristics.characteristic: title