characteristics.text: 2025-02-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Cancer Research Horizons
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18634882
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13979:Sample 18
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCC827
characteristics.characteristic: cell_line
characteristics.text: epithelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were split twice per week at the indicated timepoints. Every time a cell number corresponding to roughly 500-fold coverage of the library was pelleted, washed once with D-PBS and then stored at -20°C before processing.  Genomic DNA was extracted using the Qiagen QIAamp DNA Blood Maxi kit (order number 51194) according to the manufacturer’s protocol.   https://www.qiagen.com/de-us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/qiaamp-dna-blood-kits?catno=51194  NGS amplicon libraries were generated with a two-step PCR protocol. First, the lentiviral cassette containing the gRNA, was amplified from genomic DNA with specific primers that also contain Illumina specific adapter sequences. In the second PCR, 8 nucleotide Nextera indices were added using Indexing primers with complementary sequences to the 1st PCR primers and contained P5 and P7 Illumina compatible sequences necessary for cluster generation. Final amplicon NGS libraries were cleaned up using AMPure XP beads.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: lung adenocarcinoma
characteristics.characteristic: disease
characteristics.text: parental
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: lung
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 18
characteristics.characteristic: title