characteristics.text: 2025-02-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Institute of Animal Breeding and Genetics, Justus Liebig University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22913664
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14752:L35W
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: sheep
characteristics.characteristic: common name
characteristics.text: Protocols: Samples of liver tissue from the lobus caudatus region were collected from lambs immediately after slaughter into FixRNA (Eurx, Gdańsk, Poland) according to manufacturer’s instructions, stored for 24 h in this buffer at 4 ⁰C, and subsequently frozen in cryotubes at -80 ⁰C for long-term storage. Total RNA was extracted from the liver samples using a Universal RNA Purification Kit (Eurx, Gdańsk, Poland) according to the manufacturer’s protocol for animal tissues with the use of RNA Extracol reagent (Eurx, Gdańsk, Poland). Firstly, ribosomal RNA was removed from total RNA using TruSeq Stranded Total RNA Library Prep (Illumina, San Diego, CA, USA). RNA-seq libraries were prepared using Novogene NGS Stranded RNA Library Prep Set (PT044).
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: HLC4
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: High
characteristics.characteristic: liver copper level
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: Ovis aries
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Ovis aries
characteristics.characteristic: scientific_name
characteristics.text: L35W
characteristics.characteristic: title