2025-02-02
ENA first public
ERC000011
ENA-CHECKLIST
CNRS-UMR6290 Institut de Génétique & Développement de Rennes Université de Rennes  Equipe Expression Génétique et Développement
INSDC center name
public
INSDC status
ERS18396921
SRA accession
E-MTAB-13881:S12
Submitter Id
3
week
age
ArrayExpress
broker name
not collected
collection date
African clawed frog
common name
Protocols: Tadpoles were euthanized in 0.05% Benzocaine (Sigma), then transferred into a petri dish containing 1X HBSS (Sigma). The eyes were surgically dissected out with sharp forceps under the stereo microscope. For RNA extraction, the lens and retinal pigment epithelium were carefully removed, leaving the retinal tissue intact. The tissue was collected and kept in -80°C until use.  Total RNA was isolated from the dissected retina of the Xenopus tadpoles using TRIZOL (Invitrogen) according to the manufacturer’s protocol, followed by RNA purification with the NucleoSpin RNA mini kit (Machere-Nagel, Germany) OligodT selected mRNAs followed by Illumina mRNA stranded library preparation
description
59-62
developmental stage
Rho_crispant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Xenopus laevis
organism
Xenopus laevis
organism
retina
organism part
Xenopus laevis
scientific_name
unknown
sex
S12
title