2025-02-02
ENA first public
ERC000011
ENA-CHECKLIST
School of Medicine, University of Nottingham
INSDC center name
public
INSDC status
ERS18270904
SRA accession
E-MTAB-13803:HCT300_NEG2
Submitter Id
ArrayExpress
broker name
iPSC derived cell line
cell_line
iPSC-derived stroma
cell_type
not collected
collection date
human
common name
Protocols: Cells were washed with PBS 1x and then dissociated with TrypLE Select. Cells were labelled with anti-EpCAM magnetic beads and separated using a MACS column into EpCAM+ and EpCAM- populations. Cells were lysed with the lysis buffer (see extraction kit details) RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer’s instructions (Cat no. RTN70 - Sigma Aldrich) PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adapters
description
disease
not collected
geographic location (country and/or sea)
300k HCT116
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Co-culture
stimulus
HCT300_NEG2
title