SAMN41885059
External Id
Jiangsu University
INSDC center name
2024-06-18T00:00:00Z
INSDC first public
2024-06-20T12:01:03.233Z
INSDC last update
SRS21678502
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS21678502
SRA accession
2020-12
collection_date
For viral metagenomic analysis, fecal samples were collected from 10 bar-headed geese in December 2020 in Sakya County, Shigatse, Tibet. The fecal samples were transported to the laboratory on dry ice and stored at minus 80 degrees before use. Specimens in the specimen pool were homogenized separately using a mortar and pestle, resuspended in 1 milliliter of DPBS, and then rapidly frozen and thawed three times on dry ice. The supernatant was collected after centrifugation. The supernatant was filtered through a 0.45 micron filter (Millipore) to remove eukaryotic and bacterial cell-sized particles, and then 200 microliter of supernatant from each pool was treated with a mixture of nuclease enzymes to reduce the concentration of free (non-viral-encapsulated) nucleic acids. The remaining total nucleic acids were then isolated using the QIAamp MinElute Virus Spin Kit according to the manufacturer's protocol. One library was then constructed using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced using the Illumina NovaSeq 6000 platform with 250 bases paired ends with dual barcoding.
description
China: Shigatse City, Tibet
geo_loc_name
Anser indicus
host
feces
isolation_source
missing
lat_lon
gut metagenome
http://purl.obolibrary.org/obo/NCBITaxon_749906
organism
Metagenome or environmental sample from Fecal metagenomes of bar-headed geese
title