SAMN41940491
External Id
Curtin University of Technology
INSDC center name
2024-06-20T00:00:00Z
INSDC first public
2024-06-20T23:15:26.027Z
INSDC last update
SRS21694156
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.plant-associated
NCBI submission model
MIGS.eu.plant-associated.6.0
NCBI submission package
SRS21694156
SRA accession
2023
collection_date
Fungal DNA was randomly fragmented into ~350 bp inserts, genomic libraries were constructed using the Novogene NGS DNA Library Prep Set (Cat No.PT004) and sequenced by a PE150 strategy. Illumina reads were hard-trimmed by cutadapt v3.7 (-a AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA-A AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG --minimum-length = 50 -- max-n = 3 -n2 -q30).
description
cropland biome [ENVO_01000245]
env_broad_scale
orchard [ENVO:00000115]
env_local_scale
leaf [PO:0025034]
env_medium
boscalid+pyraclostrobin (Signum 26,7/6,7 WG; BASF), fluxapyroxad (Sercadis 30 SC; BASF), pyrimethanil (Scala 40 SC; BASF), dodine (Syllit 544 SC; UPL), copper (Cuprofix Ultra 40 WG; ADAMA) - applied during Spring season
fungicide_regm
Greece: Amfikleia, Fthiotida
geo_loc_name
Pistacia vera L.
host
>20 years
host_age
Pistachio
host_common_name
Septoria leaf spot disease
host_disease
~10m
host_height
susceptible
host_phenotype
55513
host_taxid
Trees were >20 years old, trained under standard open vase system to an average height of 10m. A standard fungicide regime was applied (see fungicide regime). Diseased leaf samples were individually transferred in moist polyethylene bags stored room temperature for 72h to induce cirrhi formation in pycnidia. Single-spored isolates were obtained by sterile loop sampling of freshly formatted cirrhus of conidia from one spot per leaf (randomly selected). Pycnidiospore suspension in 0.5 mL distilled water was spread onto Potato Dextrose Agar with 100mg/L streptomycin sulfate, incubated for 48 hr at 24 degrees Celcius in the dark, and individual germinated single-spore conidia were transferred into glass test tubes with Potato Dextrose Agar prior to DNA extraction.
isol_growth_condt
SPF4
isolate
38.629963 N 22.681427 E
lat_lon
Septoria pistaciarum
http://purl.obolibrary.org/obo/NCBITaxon_1387573
organism
MIGS Eukaryotic sample from Septoria pistaciarum
title