characteristics.text: SAMN38705028
characteristics.characteristic: External Id
characteristics.text: University of Dundee
characteristics.characteristic: INSDC center name
characteristics.text: 2025-02-01T00:51:11.930Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-02-01T00:51:11.930Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: 2021-08-03
characteristics.characteristic: collection_date
characteristics.text: Pseudomonas aeruginosa (Pa) is a key pathogen in people with cystic fibrosis (CF) and non-CF bronchiectasis. Pa lung infections are the single biggest cause of mortality in CF. During early infection, Pa adapts to the respiratory environment, leading to chronic, difficult to treat infections. Drivers of adaptation to the lung are poorly understood. Understanding Pa in-host adaptation would facilitate novel diagnosis and treatment approaches and help in the fight against accelerating antimicrobial resistance (AMR). Robust in vitro models for carrying out such studies are not available to the research community. Our previous work identified that (i) the upper airways act as a protective niche, facilitating Pa adaptation to the host , (ii) host immune pressures lead to bacterial adaptations that may influence the emergence of AMR, even in the absence of antibiotic treatment, and (iii) mutations leading to in vitro susceptibility to antibiotics may not confer susceptibility in the more relevant in vivo environments of sinuses or lungs. In vivo models do not allow investigation of sinuses and lungs in isolation, making it impossible to determine the impact of bacterial evolution within sinuses on subsequent lung infection. Currently available in vitro models are inadequate for these studies as culture conditions are a poor reflection of the respiratory tract environment. We generated and characterise culture media reproducing key features of the upper airways (sinuses) and lower airways (lungs) for the study of bacterial evolution and virulence. Experimental evolution is performed with Pa where PAO1 strain is passaged through SLM and LLM for 20 passages for 40 days. Next, we will undertake genotypic and phenotypic characterisation of passaged isolates to identify adaptive mutations and their influence on AMR and virulence.
characteristics.characteristic: description
characteristics.text: United Kingdom: Liverpool
characteristics.characteristic: geo_loc_name
characteristics.text: Experimental evolution in sputum mimic
characteristics.characteristic: isolation_source
characteristics.text: Pseudomonas aeruginosa PAO1
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_208964
characteristics.characteristic: organism
characteristics.text: Culture
characteristics.characteristic: sample_type
characteristics.text: PAO1
characteristics.characteristic: strain
characteristics.text: Pseudomonas aeruginosa PAO1 - Healthy lung media passage 20
characteristics.characteristic: title