characteristics.text: SAMN42866893
characteristics.characteristic: External Id
characteristics.text: Institute of Genetics and Developmental Biology
characteristics.characteristic: INSDC center name
characteristics.text: 2024-09-30T00:50:46.227Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-09-30T00:50:46.227Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22149334
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Plant
characteristics.characteristic: NCBI submission model
characteristics.text: Plant.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22149334
characteristics.characteristic: SRA accession
characteristics.text: 1 month
characteristics.characteristic: age
characteristics.text: 2024-04-30
characteristics.characteristic: collection_date
characteristics.text: Implementing synthetic apomixis in crops could revolutionize agricultural production by allowing clonal reproduction of elite hybrids. This process has already been demonstrated in both dicot (Arabidopsis thaliana) and monocot (rice) plants through two stages: apomeiosis and haploid induction. In our study, we explored to introduce synthetic apomixis into soybeans by initiating apomeiosis with the MiMe (Mitosis instead of Meiosis) system to produce clonal diploid gametes. We identified in soybean the five equivalents of the three key genes of the MiMe system, SPO11-1, REC8, and OSD1, and knocked out them to examine the resulting meiosis phenotypes in multiple mutants. Our findings showed that the simutaneous knockout of the SPO11-1 and REC8 orthologs triggers a switch from meiosis I to a mitosis-like division in pollen mother cells (PMCs), while OSD1 ortholog knockout partially circumvents meiosis I. We also obtained an offspring, from the soybean MiMe mutant, with a chromosome number equivalent to that of a tetraploid, but with two chromosomes fragmented. This research offers insights into the strategy of installation of apomeiosis and synthetic apomixis in soybean and paves the way for its further application.
characteristics.characteristic: description
characteristics.text: Jack
characteristics.characteristic: ecotype
characteristics.text: China:Beijing
characteristics.characteristic: geo_loc_name
characteristics.text: Glycine max
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3847
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: tissue
characteristics.text: Plant sample from Glycine max
characteristics.characteristic: title