characteristics.text: SAMN42891113
characteristics.characteristic: External Id
characteristics.text: University of Arkansas for Medical Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2024-08-02T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-02T11:58:01.679Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22154474
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS/MIMS/MIMARKS.host-associated
characteristics.characteristic: NCBI submission model
characteristics.text: MIMARKS.survey
characteristics.characteristic: NCBI submission model
characteristics.text: MIMARKS.survey.host-associated.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22154474
characteristics.characteristic: SRA accession
characteristics.text: V3V4
characteristics.characteristic: amplicon_region
characteristics.text: 7
characteristics.characteristic: cage
characteristics.text: 60 mg/kg cyclophosphamide, 4 mg/kg methotrexate, and 60 mg/kg fluorouracil
characteristics.characteristic: chem_administration
characteristics.text: 2020-06-18
characteristics.characteristic: collection_date
characteristics.text: Chemotherapy treatment, mouse 702
characteristics.characteristic: description
characteristics.text: gut(UBERON:0001007)
characteristics.characteristic: env_broad_scale
characteristics.text: colon(UBERON:0001155)
characteristics.characteristic: env_local_scale
characteristics.text: feces(UBERON:0001988)
characteristics.characteristic: env_medium
characteristics.text: CCTACGGGNGGCWGCAG
characteristics.characteristic: forward_primer
characteristics.text: USA:Arkansas,Little Rock (GAZ,00165676)
characteristics.characteristic: geo_loc_name
characteristics.text: Mus musculus (C57/BL6)
characteristics.characteristic: host
characteristics.text: Gastrointestinal
characteristics.characteristic: host_body_habitat
characteristics.text: feces
characteristics.characteristic: host_body_product
characteristics.text: ad libitum availability to drinking water and standard chow
characteristics.characteristic: host_diet
characteristics.text: standard chow
characteristics.characteristic: host_last_meal
characteristics.text: adult
characteristics.characteristic: host_life_stage
characteristics.text: female
characteristics.characteristic: host_sex
characteristics.text: 702
characteristics.characteristic: host_subject_id
characteristics.text: 10090
characteristics.characteristic: host_taxid
characteristics.text: 34.7504 N 92.3227 W
characteristics.characteristic: lat_lon
characteristics.text: mouse gut metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410661
characteristics.characteristic: organism
characteristics.text: Intraperitoneal injections on days 1, 8, 15, and 22 with 60 mg/kg cyclophosphamide, 4 mg/kg methotrexate, and 60 mg/kg fluorouracil
characteristics.characteristic: perturbation
characteristics.text: GACTACHVGGGTATCTAATCC
characteristics.characteristic: reverse_primer
characteristics.text: Fecal samples were collected from individual mice and stored at -80C. Fecal pellets were shipped to RTL Genomics (Lubbock, Texas) for DNA extraction and 16S rRNA gene sequencing using their "MiSeq Diversity Assay" targeting the V3V4 hypervariable region, with the primers (357wF: CCTACGGGNGGCWGCAG - 785R: GACTACHVGGGTATCTAATCC).
characteristics.characteristic: samp_mat_process
characteristics.text: -80 C
characteristics.characteristic: samp_store_temp
characteristics.text: V3V4 16S rRNA gene amplicon sequences were provided by RTL Genomics (Lubbock, Texas).
characteristics.characteristic: sequencing_provider
characteristics.text: CMF one-month mouse 702
characteristics.characteristic: title