2025-02-07
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112484742
External Id
INSERM Sorbonne-Universite
INSDC center name
2023-02-07T16:32:52Z
INSDC last update
public
INSDC status
ERS14602089
SRA accession
E-MTAB-12596:Res_lean_4
Submitter Id
9
week
age
ArrayExpress
broker name
tissue-resident macrophage
cell_type
house mouse
common name
Protocols: Mouse epidydimal adipose tissue was harvested and digested in Hanks Balanced Salt Solution (HBSS,no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml,Sigma-Aldrich) for 30 minutes at 37°C under agitation. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining. Cell suspensions were stained with appropriate antibodies for 30 min on ice. Cell sorting was performed on a BD FACSAria II™ cell sorter. RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen). cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).
description
adult
developmental stage
chow
diet
normal
disease
wild type genotype
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
epididymal fat pad
organism part
wild type
phenotype
Mus musculus
scientific_name
male
sex
C3H
strain
Res_lean_4
title