SAMN43024752
External Id
Agricultural Research Organisation - volcani center - Israel
INSDC center name
2024-08-04T00:00:00Z
INSDC first public
2024-08-05T10:38:40.890Z
INSDC last update
SRS22248572
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS22248572
SRA accession
2022
collection_date
Senyal, HiShtil, Israel
cultivar
Cucumber (Cucumis sativus, cv. Senyal, HiShtil, Israel) plants were cultivated under controlled conditions in a greenhouse covered with insect-proof netting (50 mesh). The plants were grown in soilless media bags containing compressed coco coir, 16 X 40 X 120 cm H:W:L (Growbags, Pelemix, Isreal). Plants were spaced 30 cm apart. A drip irrigation system was utilized to supply water containing fertilizer according to local commercial standards. To prevent infection from insect-borne plant viruses and insect infestations, the plants were sprayed once a week with a tank-mixture of two systemic insecticides: a 0.01% solution of CONFIDOR (imidacloprid-based, BAYER, Germany) and a 0.02% solution of VERIMARK (cyantraniliprole-based, DUPONT, Delaware, USA). CGMMV viral inoculum was obtained by grinding cucumber leaves from CGMMV infected plants maintained in the greenhouses at the Volcani Institute, Rishon LiZion, Israel. The leaves were grinded in sodium phosphate buffer (10 mM, pH = 7.0) as previously described by (Molad et al., 2021). Mechanical inoculation was performed by hand-rubbing sap on the three young leaves in each infected plant. This treatment was designed to recreate wounding and viral transmission during field work and harvesting (Rosemeyer et al., 1986). Infection efficiency was validated by monitoring leaf symptoms and with a PCR-based assay (Leader et al., 2021). To simulate transmission of the CGMMV in commercial agricultural practice, infection was induced by mechanical inoculation at various stages of the cultivation process, as indicated in Table 1. Each treatment included a single infection event at the mentioned growth stage. One group of 24 plants served as a control and were not inoculated
description
mature plant
dev_stage
Israel
geo_loc_name
Cucumis sativus
http://purl.obolibrary.org/obo/NCBITaxon_3659
organism
leaf
tissue
Plant sample from Cucumis sativus with no virus
title