SAMN43088609
External Id
China Pharmaceutical University
INSDC center name
2024-08-09T00:00:00Z
INSDC first public
2024-08-14T04:10:21.357Z
INSDC last update
SRS22334136
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS22334136
SRA accession
9-10 weeks
age
C57BL/6
breed
2021
collection_date
We aimed to detect the difference in immune cells after immunization of the MC38 subcutaneous transplantation model with the cancer vaccine Adpgk-NitraTh. NitraTh is a kind of helper CD4 T cell epitope containing p-nitrophenylalanine, which we have confirmed to have great anti-tumor reactivity. We used single-cell sequencing to further explore the mechanisms behind this activity, in comparison with the common helper CD4 T cell epitope PADRE. Overall, we divided the mice of the MC38 subcutaneous transplantation model into three groups, vehicle, Adpgk-PADRE, and Adpgk-NitraTh, using different vaccines to immune them. After sacrificing, we collected the tumor tissues and separated them into single-cell suspensions. Finally, we used CD45+ microbeads to collect immune cells from suspensions and sent them for single-cell sequencing.
description
China: Nanjing
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
female
sex
tumor
tissue
single cell seq for CD45+ cells from MC38 model, PADRE group
title